Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1975 Oct;2(10):1903–1910. doi: 10.1093/nar/2.10.1903

Isolation and in vitro translation of human placental lactogen messenger RNA from human term placenta.

C Hubert, L Cedard
PMCID: PMC343555  PMID: 1187349

Abstract

A messenger activity for HPL was identified in normal human term placentas. The mRNA was translated in rabbit reticulocyte cell-free system. The HPL synthesized was quantified by a specific immunoprecipitation and further identified by electrophoresis on sodium dodecyl sulfate polyacrylamide gel. The HPL synthesized in the reticulocyte lysate exhibited a molecular weight between 20,000 and 22,000 daltons similar to the active hormone. The messenger RNA activity for HPL corresponded to a sedimentation coefficient of 11-12 S. Furthermore the messenger activity for HPL was preferentially associated with membrane bound polyribosomes than with free polyribosomes.

Full text

PDF
1903

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloj S., Edelhoch H. The molecular properties of human chorionic somatomammotropin. J Biol Chem. 1971 Aug 25;246(16):5047–5052. [PubMed] [Google Scholar]
  2. Andrews P. Molecular weight of human placental lactogen investigated by gel filtration. Biochem J. 1969 Mar;111(5):799–800. doi: 10.1042/bj1110799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blobel G., Potter V. R. Ribosomes in rat liver: an estimate of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivo. J Mol Biol. 1967 Sep 28;28(3):539–542. doi: 10.1016/s0022-2836(67)80103-7. [DOI] [PubMed] [Google Scholar]
  4. Boime I., Boguslawski S., Caine J. The translation of a human placental lactogen mRNA fraction in heterologous cell-free systems: the synthesis of a possible precursor. Biochem Biophys Res Commun. 1975 Jan 6;62(1):103–109. doi: 10.1016/s0006-291x(75)80411-6. [DOI] [PubMed] [Google Scholar]
  5. Burka E. R. Characteristics of RNA degradation in the erythroid cell. J Clin Invest. 1969 Jul;48(7):1266–1272. doi: 10.1172/JCI106092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hubert C., Baliga B. S., Villee C. A., Munro H. N. Protein synthesis in a cell-free system prepared from human placenta. II. pH 5 enzyme inefficiency due to defects in tRNA charging with resulting loss of elongation factor 1. Biochim Biophys Acta. 1974 Dec 20;374(3):359–374. doi: 10.1016/0005-2787(74)90257-3. [DOI] [PubMed] [Google Scholar]
  7. Laga E. M., Baliga B. S., Munro H. N. Isolation and properties of polysomes from human placenta. Biochim Biophys Acta. 1970 Aug 8;213(2):391–400. doi: 10.1016/0005-2787(70)90047-x. [DOI] [PubMed] [Google Scholar]
  8. Lee S. Y., Mendecki J., Brawerman G. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331–1335. doi: 10.1073/pnas.68.6.1331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Palmiter R. D. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. Biochemistry. 1974 Aug 13;13(17):3606–3615. doi: 10.1021/bi00714a032. [DOI] [PubMed] [Google Scholar]
  10. Rhoads R. E., McKnight G. S., Schimke R. T. Quantitative measurement of ovalbumin messenger ribonucleic acid activity. Localization in polysomes, induction by estrogen, and effect of actinomycin D. J Biol Chem. 1973 Mar 25;248(6):2031–2039. [PubMed] [Google Scholar]
  11. Saxena B. N., Emerson K., Jr, Selenkow H. A. Serum placental lactogen (HPL) levels as an index of placental function. N Engl J Med. 1969 Jul 31;281(5):225–231. doi: 10.1056/NEJM196907312810501. [DOI] [PubMed] [Google Scholar]
  12. Spellacy W. N., Buhi W. C., Birk S. A. The effectiveness of human placental lactogen measurements as an adjunct in decreasing perinatal deaths. Results of a retrospective and a randomized controlled prospective study. Am J Obstet Gynecol. 1975 Mar 15;121(6):835–844. [PubMed] [Google Scholar]
  13. Suwa S., Friesen H. Biosynthesis of human placental proteins and human placental lactogen (HPL) in vitro. II. Dynamic studies of normal term placentas. Endocrinology. 1969 Dec;85(6):1037–1045. doi: 10.1210/endo-85-6-1037. [DOI] [PubMed] [Google Scholar]
  14. Tyson J. E., Austin K. L., Farinholt J. W. Prolonged nutritional deprivation in pregnancy: changes in human chorionic somatomammotropin and growth hormone secretion. Am J Obstet Gynecol. 1971 Apr 1;109(7):1080–1082. doi: 10.1016/0002-9378(71)90293-6. [DOI] [PubMed] [Google Scholar]
  15. Ver Eecke T., Vanduffel L., Peeters B., Rombauts W. The synthesis of human placental lactogen by human placental ribosomes. Arch Int Physiol Biochim. 1974 Dec;82(5):1024–1026. [PubMed] [Google Scholar]
  16. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES