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. 1975 Nov;2(11):2021–2036. doi: 10.1093/nar/2.11.2021

Specific binding of the first enzyme for histidine biosynthesis to the DNA of histidine operon.

M Meyers, F Blasi, C B Bruni, R G Deeley, J S Kovach, M Levinthal, K P Mullinix, T Vogel, R F Goldberger
PMCID: PMC343569  PMID: 1052526

Abstract

Studies were done to examine direct binding of the first enzyme of the histidine biosynthetic pathway (phosphoribosyltransferase) to 32P-labeled phi80dhis DNA and competition of this binding by unlabeled homologous DNA and by various preparations of unlabeled heterologous DNA, including that from a defective phi80 bacteriophage carrying the histidine operon with a deletion of part of its operator region. Our findings show that phosphoribosyltransferase binds specifically to site in or near the regulatory region of the histidine operon. The stability of the complex formed by interaction of the enzyme with the DNA was markedly decreased by the substrates of the enzyme and was slightly increased by the allosteric inhibitor, histidine. These findings are consistent with previous data that indicate that phosphoribosyltransferase plays a role in regulating expression of the histidine operon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., GARRY B., HERZENBERG L. A. The genetic control of the enzymes of histidine biosynthesis in Salmonella typhimurium. J Gen Microbiol. 1960 Apr;22:369–378. doi: 10.1099/00221287-22-2-369. [DOI] [PubMed] [Google Scholar]
  2. AMES B. N., HARTMAN P. E., JACOB F. Chromosomal alterations affecting the regulation of histidine biosynthetic enzymes in Salmonella. J Mol Biol. 1963 Jul;7:23–42. doi: 10.1016/s0022-2836(63)80016-9. [DOI] [PubMed] [Google Scholar]
  3. AMES B. N., MARTIN R. G., GARRY B. J. The first step of histidine biosynthesis. J Biol Chem. 1961 Jul;236:2019–2026. [PubMed] [Google Scholar]
  4. ATKINSON D. E., HATHAWAY J. A., SMITH E. C. KINETICS OF REGULATORY ENZYMES. KINETIC ORDER OF THE YEAST DIPHOSPHOPYRIDINE NUCLEOTIDE ISOCITRATE DEHYDROGENASE REACTION AND A MODEL FOR THE REACTION. J Biol Chem. 1965 Jun;240:2682–2690. [PubMed] [Google Scholar]
  5. Ames B. N., Garry B. COORDINATE REPRESSION OF THE SYNTHESIS OF FOUR HISTIDINE BIOSYNTHETIC ENZYMES BY HISTIDINE. Proc Natl Acad Sci U S A. 1959 Oct;45(10):1453–1461. doi: 10.1073/pnas.45.10.1453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Antón D. N. Histidine regulatory mutants in Salmonella typhimurium. V. Two new classes histidine regulatory mutants. J Mol Biol. 1968 May 14;33(3):533–546. doi: 10.1016/0022-2836(68)90304-5. [DOI] [PubMed] [Google Scholar]
  7. Bell R. M., Parsons S. M., Dubravac S. A., Redfield A. G., Koshland D. E., Jr Characterization of slowly interconvertible states of phosphoribosyladenosine triphosphate synthetase dependent on temperature, substrates, and histidine. J Biol Chem. 1974 Jul 10;249(13):4110–4118. [PubMed] [Google Scholar]
  8. Blasi F., Aloj S. M., Goldberger R. F. Effect of histidine on the enzyme which catalyzes the first step of histidine biosynthesis in Salmonella typhimurium. Biochemistry. 1971 Apr 13;10(8):1409–1417. doi: 10.1021/bi00784a021. [DOI] [PubMed] [Google Scholar]
  9. Blasi F., Barton R. W., Kovach J. S., Goldberger R. F. Interaction between the first enzyme for histidine biosynthesis and histidyl transfer ribonucleic acid. J Bacteriol. 1971 May;106(2):508–513. doi: 10.1128/jb.106.2.508-513.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Blasi F., Bruni C. B., Avitabile A., Deeley R. G., Goldberger R. F., Meyers M. M. Inhibition of transcription of the histidine operon in vitro by the first enzyme of the histidine pathway. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2692–2696. doi: 10.1073/pnas.70.9.2692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goldberger R. F., Kovach J. S. Regulation of histidine biosynthesis in Salmonella typhimurium. Curr Top Cell Regul. 1972;5:285–308. doi: 10.1016/b978-0-12-152805-8.50014-9. [DOI] [PubMed] [Google Scholar]
  12. HARTMAN P. E., HARTMAN Z., SERMAN D. Complementation mapping by abortive transduction of histidine requiring Salmonella mutants. J Gen Microbiol. 1960 Apr;22:354–368. doi: 10.1099/00221287-22-2-354. [DOI] [PubMed] [Google Scholar]
  13. Hartman P. E., Hartman Z., Stahl R. C. Classification and mapping of spontaneous and induced mutations in the histidine operon of Salmonella. Adv Genet. 1971;16:1–34. doi: 10.1016/s0065-2660(08)60352-1. [DOI] [PubMed] [Google Scholar]
  14. Jackson E. N., Yanofsky C. Thr region between the operator and first structural gene of the tryptophan operon of Escherichia coli may have a regulatory function. J Mol Biol. 1973 May 5;76(1):89–101. doi: 10.1016/0022-2836(73)90082-x. [DOI] [PubMed] [Google Scholar]
  15. Kovach J. S., Ballesteros A. O., Meyers M., Soria M., Goldberger R. F. A cis-trans test of the effect of the first enzyme for histidine biosynthesis on regulation of the histidine operon. J Bacteriol. 1973 Apr;114(1):351–356. doi: 10.1128/jb.114.1.351-356.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kovach J. S., Berberich M. A., Venetianer P., Goldberger R. F. Repression of the histidine operon: effect of the first enzyme on the kinetics of repression. J Bacteriol. 1969 Mar;97(3):1283–1290. doi: 10.1128/jb.97.3.1283-1290.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kovach J. S., Phang J. M., Blasi F., Barton R. W., Ballesteros-Olmo A., Goldberger R. F. Interaction between histidyl transfer ribonucleic acid and the first enzyme for histidine biosynthesis of Salmonella typhimurium. J Bacteriol. 1970 Nov;104(2):787–792. doi: 10.1128/jb.104.2.787-792.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kovach J. S., Phang J. M., Ference M., Goldberger R. F. Studies on repression of the histidine operon. II. The role of the first enzyme in control of the histidine system. Proc Natl Acad Sci U S A. 1969 Jun;63(2):481–488. doi: 10.1073/pnas.63.2.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LEVIN A. P., HARTMAN P. E. ACTION OF A HISTIDINE ANALOGUE, 1,2,4-TRIAZOLE-3-ALANINE, IN SALMONELLA TYPHIMURIUM. J Bacteriol. 1963 Oct;86:820–828. doi: 10.1128/jb.86.4.820-828.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. LOPER J. C., GRABNAR M., STAHL R. C., HARTMAN Z., HARTMAN P. E. GENES AND PROTEINS INVOLVED IN HISTIDINE BIOSYNTHESIS IN SALMONELLA. Brookhaven Symp Biol. 1964 Dec;17:15–52. [PubMed] [Google Scholar]
  21. Lewis J. A., Ames B. N. Histidine regulation in Salmonella typhimurium. XI. The percentage of transfer RNA His charged in vivo and its relation to the repression of the histidine operon. J Mol Biol. 1972 Apr 28;66(1):131–142. doi: 10.1016/s0022-2836(72)80011-1. [DOI] [PubMed] [Google Scholar]
  22. Lin S. Y., Riggs A. D. Lac repressor binding to non-operator DNA: detailed studies and a comparison of eequilibrium and rate competition methods. J Mol Biol. 1972 Dec 30;72(3):671–690. doi: 10.1016/0022-2836(72)90184-2. [DOI] [PubMed] [Google Scholar]
  23. MOYED H. S. Interference with the feed-back control of histidine biosynthesis. J Biol Chem. 1961 Aug;236:2261–2267. [PubMed] [Google Scholar]
  24. MOYED H. S., MAGASANIK B. The biosynthesis of the imidazole ring of histidine. J Biol Chem. 1960 Jan;235:149–153. [PubMed] [Google Scholar]
  25. Patthy L., Dénes G. Altered repression behaviour in a feedback insensitive mutant of Escherichia coli K12. Acta Biochim Biophys Acad Sci Hung. 1970;5(2):147–157. [PubMed] [Google Scholar]
  26. Riggs A. D., Suzuki H., Bourgeois S. Lac repressor-operator interaction. I. Equilibrium studies. J Mol Biol. 1970 Feb 28;48(1):67–83. doi: 10.1016/0022-2836(70)90219-6. [DOI] [PubMed] [Google Scholar]
  27. Roth J. R., Ames B. N. Histidine regulatory mutants in Salmonella typhimurium II. Histidine regulatory mutants having altered histidyl-tRNA synthetase. J Mol Biol. 1966 Dec 28;22(2):325–333. doi: 10.1016/0022-2836(66)90135-5. [DOI] [PubMed] [Google Scholar]
  28. Roth J. R., Antón D. N., Hartman P. E. Histidine regulatory mutants in Salmonella typhimurium. I. Isolation and general properties. J Mol Biol. 1966 Dec 28;22(2):305–323. doi: 10.1016/0022-2836(66)90134-3. [DOI] [PubMed] [Google Scholar]
  29. Roth J. R., Hartman P. E. Heterogeneity in P22 transducing particles. Virology. 1965 Nov;27(3):297–307. doi: 10.1016/0042-6822(65)90109-1. [DOI] [PubMed] [Google Scholar]
  30. Roth J. R., Sanderson K. E. Orientation of the isoleucine-valine genes in the Salmonella typhimurium linkage map. Genetics. 1966 May;53(5):971–976. doi: 10.1093/genetics/53.5.971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Rothman-Denes L., Martin R. G. Two mutations in the first gene of the histidine operon of Salmonella typhimurium affecting control. J Bacteriol. 1971 Apr;106(1):227–237. doi: 10.1128/jb.106.1.227-237.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. SMITH D. W., AMES B. N. INTERMEDIATES IN THE EARLY STEPS OF HISTIDINE BIOSYNTHESIS. J Biol Chem. 1964 Jun;239:1848–1855. [PubMed] [Google Scholar]
  33. SMITH D. W., AMES B. N. PHOSPHORIBOSYLADENOSINE MONOPHOSPHATE, AN INTERMEDIATE IN HISTIDINE BIOSYNTHESIS. J Biol Chem. 1965 Jul;240:3056–3063. [PubMed] [Google Scholar]
  34. Silbert D. F., Fink G. R., Ames B. N. Histidine regulatory mutants in Salmonella typhimurium 3. A class of regulatory mutants deficient in tRNA for histidine. J Mol Biol. 1966 Dec 28;22(2):335–347. doi: 10.1016/0022-2836(66)90136-7. [DOI] [PubMed] [Google Scholar]
  35. Smith G. R., Tong B. Construction of phi80 dhis carrying Salmonella typhimurium histidine operon mutations. J Bacteriol. 1974 Dec;120(3):1223–1226. doi: 10.1128/jb.120.3.1223-1226.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Smith O., Meyers M. M., Vogel T., Deeley R. D., Goldberger R. Defective in vitro binding of histidyl-transfer ribonucleic acid to feedback resistant phosphoribosyl transferase of Salmonella typhimurium. Nucleic Acids Res. 1974 Jul;1(7):881–888. doi: 10.1093/nar/1.7.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Smolarsky M., Tal M. Novel method for measuring polyuridylic acid binding to ribosomes. Biochim Biophys Acta. 1970 Feb 18;199(2):447–452. doi: 10.1016/0005-2787(70)90087-0. [DOI] [PubMed] [Google Scholar]
  38. Somerville R. L., Stetson H. Expression of the tryptophan operon in merodiploids of Escherichia coli. II. Effects of polar mutations in the trpE gene. Mol Gen Genet. 1974;131(3):247–261. doi: 10.1007/BF00267964. [DOI] [PubMed] [Google Scholar]
  39. Venetianer P. Level of messenger RNA transcribed from the histidine operon in repressed, derepressed and histidine-starved Salmonella typhimurium. J Mol Biol. 1969 Oct 28;45(2):375–384. doi: 10.1016/0022-2836(69)90112-0. [DOI] [PubMed] [Google Scholar]
  40. Vogel T., Meyers M., Kovach J. S., Goldberger R. F. Specificity of interaction between the first enzyme for histidine biosynthesis and aminoacylated histidine transfer ribonucleic acid. J Bacteriol. 1972 Oct;112(1):126–130. doi: 10.1128/jb.112.1.126-130.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Whitfield H. J., Jr Purification and properties of the wild type and a feedback-resistant phosphoribosyladenosine triphosphate pyrophosphate phosphoribosyltransferase, the first enzyme of histidine biosynthesis in Salmonella typhimurium. J Biol Chem. 1971 Feb 25;246(4):899–908. [PubMed] [Google Scholar]

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