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. 1975 Nov;2(11):2069–2075. doi: 10.1093/nar/2.11.2069

Primary structure of tRNA-Lys of E. coli B.

K Chakraburtty, A Steinschneider, R V Case, A H Mehler
PMCID: PMC343573  PMID: 802509

Abstract

The primary structure of tRNALys of E. coli was determined by use of [32P]-tRNA. The sequence is pGGGUCGUUAGCUCAGDDGGDAGAGCAGUUGACUmam5-s2-UUU-t6AApsiCAAUUGm7GXCGCAGGTpsiCGAAUCCUGCACGACCCACCA. No s4-U was detected in position 8. No other lysine tRNA was detected but the existence of another species has not been ruled out.

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Selected References

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  1. Gillam I., Blew D., Warrington R. C., von Tigerstrom M., Tener G. M. A general procedure for the isolation of specific transfer ribonucleic acids. Biochemistry. 1968 Oct;7(10):3459–3468. doi: 10.1021/bi00850a022. [DOI] [PubMed] [Google Scholar]
  2. HOLLEY R. W., APGAR J., EVERETT G. A., MADISON J. T., MARQUISEE M., MERRILL S. H., PENSWICK J. R., ZAMIR A. STRUCTURE OF A RIBONUCLEIC ACID. Science. 1965 Mar 19;147(3664):1462–1465. doi: 10.1126/science.147.3664.1462. [DOI] [PubMed] [Google Scholar]
  3. Ishikura H., Yamada Y., Murao K., Saneyoshi M., Nishimura S. The presence of N-[9-(beta-D-ribofuranosyl)purin-6-ylcarbamoyl]threonine in serine, methionine and lysine transfer RNA's from Escherichia coli. Biochem Biophys Res Commun. 1969 Dec 4;37(6):990–995. doi: 10.1016/0006-291x(69)90229-0. [DOI] [PubMed] [Google Scholar]
  4. Madison J. T., Boguslawski S. J., Teetor G. H. Nucleotide sequence of a lysine transfer ribonucleic Acid from bakers' yeast. Science. 1972 May 12;176(4035):687–689. doi: 10.1126/science.176.4035.687. [DOI] [PubMed] [Google Scholar]
  5. Mehler A. H., Chakraburtty K. Some questions about the structure and activity of aminoacyl-tRNA synthetases. Adv Enzymol Relat Areas Mol Biol. 1971;35:443–501. doi: 10.1002/9780470122808.ch8. [DOI] [PubMed] [Google Scholar]
  6. Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
  7. Oashi Z., Saneyoshi M., Harada F., Hara H., Nishimura S. Presumed anticodon structure of glutamic acid tRNA from E. coli: a possible location of a 2-thiouridine derivative in the first position of the anticodon. Biochem Biophys Res Commun. 1970 Aug 24;40(4):866–872. doi: 10.1016/0006-291x(70)90983-6. [DOI] [PubMed] [Google Scholar]
  8. Remy P., Birmelé C., Ebel J. P. Purification of yeast phenylalanyl-tRNA synthetase by affinity chromatography, on a tRNA(Phe)-sepharose column. FEBS Lett. 1972 Oct 15;27(1):134–138. doi: 10.1016/0014-5793(72)80426-5. [DOI] [PubMed] [Google Scholar]
  9. Smith C. J., Ley A. N., D'Obrenan P., Mitra S. K. The structure and coding specificity of a lysine transfer ribonucleic acid from the haploid yeast Saccharomyces cerevisiae alpha S288C. J Biol Chem. 1971 Dec 25;246(24):7817–7819. [PubMed] [Google Scholar]
  10. Steinschneider A. Thermal chromatography of lysine-specific transfer ribonucleic acid from Escherichia coli B. J Chromatogr. 1975 Jan 22;103(2):355–363. doi: 10.1016/s0021-9673(00)87227-1. [DOI] [PubMed] [Google Scholar]
  11. Stern R., Mehler A. H. Lysyl-sRNA synthetase from Escherichia coli. Biochem Z. 1965 Aug 19;342(4):400–409. [PubMed] [Google Scholar]
  12. TOMLINSON R. V., TENER G. M. THE EFFECT OF UREA, FORMAMIDE, AND GLYCOLS ON THE SECONDARY BINDING FORCES IN THE ION-EXCHANGE CHROMATOGRAPHY OF POLYNUCLEOTIDES OF DEAE-CELLULOSE. Biochemistry. 1963 Jul-Aug;2:697–702. doi: 10.1021/bi00904a013. [DOI] [PubMed] [Google Scholar]

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