Abstract
The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.
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Selected References
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- De Groot N., Panet A., Lapidot Y. The binding of purified Phe-tRNA and peptidyl-tRNA Phe to Escherichia coli ribosomes. Eur J Biochem. 1971 Dec 10;23(3):523–527. doi: 10.1111/j.1432-1033.1971.tb01649.x. [DOI] [PubMed] [Google Scholar]
- Friedman S. Acylation of transfer ribonucleic acid with the N-hydroxysuccinimide ester of phenoxyacetic acid. Biochemistry. 1972 Aug 29;11(18):3435–3443. doi: 10.1021/bi00768a017. [DOI] [PubMed] [Google Scholar]
- Friedman S. Patterns of base modification in tRNA. Nat New Biol. 1973 Jul 4;244(131):18–20. doi: 10.1038/newbio244018a0. [DOI] [PubMed] [Google Scholar]
- Gillam I., Blew D., Warrington R. C., von Tigerstrom M., Tener G. M. A general procedure for the isolation of specific transfer ribonucleic acids. Biochemistry. 1968 Oct;7(10):3459–3468. doi: 10.1021/bi00850a022. [DOI] [PubMed] [Google Scholar]
- Gillam I., Millward S., Blew D., von Tigerstrom M., Wimmer E., Tener G. M. The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. Biochemistry. 1967 Oct;6(10):3043–3056. doi: 10.1021/bi00862a011. [DOI] [PubMed] [Google Scholar]
- Lapidot Y., De Groot N., Rappoport S., Elat D. Peptidyl transfer RNA. 8. The chemical synthesis of glycine and alanine containing oligopeptidyl transfer RNA. Biochim Biophys Acta. 1969 Oct 22;190(2):304–311. doi: 10.1016/0005-2787(69)90081-1. [DOI] [PubMed] [Google Scholar]
- Lapidot Y., Elat D., Rappoport S., de Groot N. Peptidyl-tRNA. IX. The use of o-nitrophenylsulfenyl group as N-protecting group in the synthesis of peptidyl-tRNA. Biochem Biophys Res Commun. 1970 Feb 20;38(4):559–563. doi: 10.1016/0006-291x(70)90617-0. [DOI] [PubMed] [Google Scholar]
- Lapidot Y., Rappoport S. The synthesis of oligopeptidyl-tRNA. Methods Enzymol. 1974;29:688–695. doi: 10.1016/0076-6879(74)29061-x. [DOI] [PubMed] [Google Scholar]
- Lapidot Y., de Groot N. The chemical synthesis and the biochemical properties of peptidyl-tRNA. Prog Nucleic Acid Res Mol Biol. 1972;12:189–228. doi: 10.1016/s0079-6603(08)60663-7. [DOI] [PubMed] [Google Scholar]
- Merrifield R. B. Solid-phase peptide synthesis. Adv Enzymol Relat Areas Mol Biol. 1969;32:221–296. doi: 10.1002/9780470122778.ch6. [DOI] [PubMed] [Google Scholar]
- Rahamimoff H., Baksht E., Lapidot Y., de Groot N. The binding of Phe-tRNA(Phe) and gly(2)Phe-tRNA(Phe) to reticulocyte ribosomal peptidyl sites by a mechanism not involving translocation. FEBS Lett. 1972 May 1;22(2):249–251. doi: 10.1016/0014-5793(72)80056-5. [DOI] [PubMed] [Google Scholar]
- Roy K. L., Söll D. Fractionation of Escherichia coli transfer RNA on benzoylated DEAE-cellulose. Biochim Biophys Acta. 1968 Jul 23;161(2):572–574. doi: 10.1016/0005-2787(68)90137-8. [DOI] [PubMed] [Google Scholar]
- Schofield P., Hoffman B. M., Rich A. Spin-labeling studies of aminoacyl transfer ribonucleic acid. Biochemistry. 1970 Jun 9;9(12):2525–2533. doi: 10.1021/bi00814a020. [DOI] [PubMed] [Google Scholar]
- Sokolff L., Rappaport H. P. Isolation of isoaccepting tRNAs. Arch Biochem Biophys. 1972 Dec;153(2):788–796. doi: 10.1016/0003-9861(72)90400-6. [DOI] [PubMed] [Google Scholar]
- Strauss J. H., Jr, Kelly R. B., Sinsheimer R. L. Denaturation of RNA with dimethyl sulfoxide. Biopolymers. 1968 Jun;6(6):793–807. doi: 10.1002/bip.1968.360060604. [DOI] [PubMed] [Google Scholar]
- Ziv E., De Groot N., Lapidot Y. Peptidyl-tRNA. XII. Behaviour of peptidyl-tRNA-Phe from Escherichia coli on methylated albumin silicic acid column. Biochim Biophys Acta. 1971 Jan 1;228(1):135–140. [PubMed] [Google Scholar]
