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. 1975 Dec;2(12):2355–2364. doi: 10.1093/nar/2.12.2355

A modified procedure for the preparation of di- and triribonucleotides from pancreatic ribonuclease digest of RNA.

S Bauer, M D White, Y Lapidot
PMCID: PMC343602  PMID: 1052545

Abstract

A novel procedure for the separation of oligonucleotides from pancreatic RNase-digest of RNA is described. The method involves a group-separation of uracil-containing and of cytosine-containing nucleotides on Dowex 50W. The obtained groups are further separated on DEAE-Sephadex A-25 by a linear gradient of NH4HCO3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoyagi S., Inoue Y. Oligonucleotide studies. I. Isolation of dinucleotides and seven trinucleotides from ribonuclease T-1 digests of nucleic acids and their ultraviolet spectral characterizations. J Biol Chem. 1968 Feb 10;243(3):514–520. [PubMed] [Google Scholar]
  2. Aoyagi S., Inoue Y. Oligonucleotide studies. VI. Large scale fractionation of di- and trinucleotides obtained from pancreatic ribonuclease digests. J Biochem. 1968 Nov;64(5):603–609. doi: 10.1093/oxfordjournals.jbchem.a128937. [DOI] [PubMed] [Google Scholar]
  3. Högenauer G. The stability of a codon transfer RNA complex. Eur J Biochem. 1970 Feb;12(3):527–532. doi: 10.1111/j.1432-1033.1970.tb00882.x. [DOI] [PubMed] [Google Scholar]
  4. KATZ S., COMB D. G. A NEW METHOD FOR THE DETERMINATION OF THE BASE COMPOSITION OF RIBONUCLEIC ACID. J Biol Chem. 1963 Sep;238:3065–3067. [PubMed] [Google Scholar]
  5. Lohrmann R., Söll D., Hayatsu H., Ohtsuka E., Khorana H. G. Studies on polynucleotides. LI. Syntheses of the 64 possible ribotrinucleotides derived from the four major ribomononucleotides. J Am Chem Soc. 1966 Feb 20;88(4):819–829. doi: 10.1021/ja00956a039. [DOI] [PubMed] [Google Scholar]
  6. NIRENBERG M., LEDER P. RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES. Science. 1964 Sep 25;145(3639):1399–1407. doi: 10.1126/science.145.3639.1399. [DOI] [PubMed] [Google Scholar]
  7. Satoh K., Inoue Y. Ion-exchangechrmtography of a dinucleotide preparation from controlled alkaline hydrolysis of ribonucleic acids. Biochem J. 1969 Sep;114(2):271–277. doi: 10.1042/bj1140271. [DOI] [PMC free article] [PubMed] [Google Scholar]

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