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. 2011 Nov 11;104(1):34–47. doi: 10.1111/boc.201100081

Construction of a Plasmodium falciparum Rab-interactome identifies CK1 and PKA as Rab-effector kinases in malaria parasites

Fathia Ben Rached *,†,‡,1, Carinne Ndjembo-Ezougou *,†,‡,1, Syama Chandran §,1, Hana Talabani *,†,‡, Hélène Yera *,†, Vrushali Dandavate §, Pierre Bourdoncle *,†,‡, Markus Meissner , Utpal Tatu §, Gordon Langsley *,†,‡,2
PMCID: PMC3437490  PMID: 22188458

Abstract

Background information

The pathology causing stages of the human malaria parasite Plasmodium falciparum reside within red blood cells that are devoid of any regulated transport system. The parasite, therefore, is entirely responsible for mediating vesicular transport within itself and in the infected erythrocyte cytoplasm, and it does so in part via its family of 11 Rab GTPases. Putative functions have been ascribed to Plasmodium Rabs due to their homology with Rabs of yeast, particularly with Saccharomyces that has an equivalent number of rab/ypt genes and where analyses of Ypt function is well characterized.

Results

Rabs are important regulators of vesicular traffic due to their capacity to recruit specific effectors. In order to identify P. falciparum Rab (PfRab) effectors, we first built a Ypt-interactome by exploiting genetic and physical binding data available at the Saccharomyces genome database (SGD). We then constructed a PfRab-interactome using putative parasite Rab-effectors identified by homology to Ypt-effectors. We demonstrate its potential by wet-bench testing three predictions; that casein kinase-1 (PfCK1) is a specific Rab5B interacting protein and that the catalytic subunit of cAMP-dependent protein kinase A (PfPKA-C) is a PfRab5A and PfRab7 effector.

Conclusions

The establishment of a shared set of physical Ypt/PfRab-effector proteins sheds light on a core set Plasmodium Rab-interactants shared with yeast. The PfRab-interactome should benefit vesicular trafficking studies in malaria parasites. The recruitment of PfCK1 to PfRab5B+ and PfPKA-C to PfRab5A+ and PfRab7+ vesicles, respectively, suggests that PfRab-recruited kinases potentially play a role in early and late endosome function in malaria parasites.

Keywords: Interactom, Kinase, Plasmodium, Rab, Yeast, Ypt

Introduction

There are more than 225 million cases of malaria each year that result in 800000 deaths (http://www.who.int/malaria/world_malaria_report_2010/en/index.html). One reason that this apicomplexa parasite is such a successful pathogen is that it resides within red bloods cells that are MHC class I and class II negative and so are unable to present parasite-derived peptides to the human immune system. The phylum apicomplexa has a number of medically important pathogens that include Theileria and Babesia that, such as Plasmodium, invade and develop within host red blood cells and subsequently modifies them (Plattner and Soldati-Favre, 2008). Mature erythrocytes do not have a nucleus and indeed, lack regulated vesicular transport. In spite of this, the intracellular parasite is able to secrete antigen and metabolic waste such as lactate and import nutrients, such as glucose and haemoglobin (Langsley et al., 2008). Like yeast, Plasmodium has to import and export substances to and from its own organelles (Kats et al., 2008) and across its own parasite plasma membrane. In addition, it also faces the challenge of importing and exporting molecules across the parasitophorous vacuole membrane within which it resides and across the infected host cell plasma membrane (Hanssen et al., 2010; Gruring et al., 2011). Little is known about how the parasite regulates the various secretory and import pathways, but likely candidates to play a key role are Rab GTPases due to their established role in regulating vesicular transport in other eukaryotes (Stenmark, 2009; Goud and Gleeson, 2010).

Plasmodium parasites have a family of 11 different Rabs (Quevillon et al., 2003). The number of Rabs present in other apicomplexa vary, with Theileria and Babesia having just 9, Cryptosporidium 8, and Toxoplasma 15 (Langsley et al., 2008). The reason why the number of individual Rabs varies between different organisms is unknown. However, recent detailed phylogenetic analysis indicates that Rab11B is restricted to alveolates that include apicomplexa, whereas Rab11A orthologues are widespread in eukaryotes (Agop-Nersesian et al., 2010). Consistently, apicomplexa Rab11B is involved in an alveolate-specific function; namely, the biogenesis of a cytoskeleton-like structure called the Inner Membrane Complex (IMC), whereas Rab11A (Langsley and Chakrabarti, 1996) performs a more classical role in parasite cytokinesis (Agop-Nersesian et al., 2009). The vesicular membrane association of a Rab is due to its C-terminal prenylation at a CC or CXC motif and the P. falciparum enzyme (geranylgeranyl transferase-1) responsible for this lipid modification has been described (Chakrabarti et al., 1998). The recycling of vesicles from the donor to acceptor membrane involves a transport protein called rabGDI that binds a Rab in its GDP form, and two Plasmodium rabGDIs have been identified (Attal and Langsley, 1996). Plasmodium, therefore, appears to possesses the basic machinery associated with recycling of Rab proteins. However, Rabs are important regulators of vesicular traffic due to their capacity to recruit specific effectors (Stenmark, 2009), and to date the only specific Plasmodium Rab-effector protein to be described is a myosin light chain called MTIP (Agop-Nersesian et al., 2009). Homology searches using Rab-effectors from higher eukaryotes such as the well-known Rab11 effectors FIP (Horgan and McCaffrey, 2009) have failed to identify orthologues.

Rabs in the yeast Saccharomyces cerevisiae are mostly referred to as Ypt proteins and their role in regulating vesicular traffic has been well studied both genetically and biochemically for many years (Segev, 2001). A regularly updated body of data is collated and readily accessible via the Saccharaomyces genome database (SGD: http://www.yeastgenome.org). Given that both S. cerevisiae and P. falciparum are haploid unicellular lower eukaryotes, we decided to exploit the wealth of data on Ypt effectors with the goal of identifying parasite-specific Rab-effectors homologous with yeast. Surprisingly, no Ypt-interactome has been previously reported, so we constructed one and then used it to build a P. falciparum Rab (PfRab) interactome. The parasite Rab-interactome allowed us to predict and then demonstrate in vitro the physical interaction between PfRab5B and Casein kinase 1 (PfCK1) (Barik et al., 1997), and between PfRab5A and PfRab7 and the catalytic subunit of protein kinase A (PfPKA-C) (Syin et al., 2001). We also provide evidence that in vivo, the degree of interaction between PfRab5A and PfRab7 with PfPKA-C changes as the parasite develops to a multinucleated schizont within the red blood cell. These physical interactions suggest a mechanism of recruitment of PfCK1 to PfRab5B+ compartments and PfPKA to PfRab5A+ and PfRab7+ vesicles. In addition to identifying PfPKA as a parasite Rab5A- and Rab7-effector protein, our study suggests a possible role for cAMP-dependent protein kinase activity in regulating PfRab5A- and PfRab7-mediated vesicular transport in malaria parasites.

Results

Construction of an Ypt-interactome

All interactants catalogued at SGD as having been demonstrated to either genetically, or physically interact with a given Ypt protein were downloaded and an interactome built for the whole Ypt family and for each individual Ypt protein (see Supplementary Table S1 for all Ypt-interactants). As the global (genetic plus physical) Ypt-interactome is complicated to a point of being visibly difficult to interpret it is not shown, and Figure 1A shows only the interactome based on physical interactions with a given Ypt.

Figure 1. Representation of Physical Ypt/Rab interactions in S. cerevisiae and P. falciparum.

Figure 1

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(A) Physical interactome of the Ypt proteins present in S. cerevisiae. (B) Physical interactome of PfRab proteins. Red represents Rab proteins and pink indicates the interacting protein. Green indicates effector proteins that physically interact with three or more Ypt/Rab proteins. The putative interaction between PfCK1 and the three PfRab5s is shown in a pink dotted line due to difficulties in assigning a specific PfRab5 isoform.

Specific Ypt-interactomes based on all genetic and physical interactions are shown in Figure 2. The Ypt6 network (Figure 2A) displays a remarkable complexity of interactions that clearly underscores the central role that Ypt6 plays in regulating vesicular transport in yeast. However, not all Ypt proteins display the same level of interactions with Ypt1 having a less complicated network (Figure 2B) and that of Ypt32 being even more reduced (Figure 2C). Individual physical interactomes for six other Ypt proteins are displayed in Supplementary Figure S1. As the range of potential processes involving a given Ypt/Rab is determined in part by the panoply of its specific interactants, it implies that Ypt10 and Ypt11 (Supplementary Figures S1B and S1C) regulate a restricted number of events, in contrast to Ypt1 (Supplementary Figures S1A and S1D) that has the capacity to mediate many.

Figure 2. Specific Ypt-interactomes based on all genetic and physical interactions.

Figure 2

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(A) Complex interacting network of YPT6, which has more than 100 interacting proteins that include YPT7, YPT1 and VPS21. (B) Interactome network of YPT1. (C) Representation of a simpler network that shows interacting proteins of YPT32. Red represents Rab proteins and pink indicates the interacting protein.

PfRab homology and orthology assignments

Multiple reciprocal BLAST analyses has given rise to the PfRab nomenclature (Quevillon et al., 2003) and identified the corresponding homologue in S. cerevisiae (Table 1 and Supplementary Figure S2). However, many new Rab sequences from different organisms have since been deposited into public databases and at OrthoMCL DB (http://orthomcl.org) collated into orthology groups. Interrogation of OrthoMCL allows one to identify the orthology group for given PfRab (Table 2). Certain orthology groups are restricted, such as the Rab11B group (OG4_21991) that contains only alveolates, as already mentioned (Agop-Nersesian et al., 2010). PfRab5A belongs to the most restricted orthology group (OG4_36791) that only contains apicomplexa parasites (Plasmodia, Babesia and Theileria) known to invade erythrocytes. The PfRab5B/Ypt52 orthology group (OG4_18709) is less restricted containing six species, whereas the PfRab5C/Ypt51 (OG4_10168) orthology group is the largest. We built PfRab-interactomes based exclusively on Ypt-Rab pairs that belong to the same orthology group (Table 2). However, for the three PfRab5s we constructed a single interactome, since sequence similarities between these and the yeast Rab5 isoforms did not allow individual isoform-specific orthology assignments to be made with confidence.

Table 1.

Plasmodium falciparum homologues of S. cerevisiae Ypt/Rabs

Serial no. S. cerevisiae protein P. falciparum protein
1 SEC4/SRO6 PfRab1A (PFE0690c)
2 YPT1/YP2 PfRab1B (PFE0625w)
3 YPT10 PfRab18 (PF08_0110)
4 YPT11 PfRab11B (MAL13P1.205)
5 YPT31/YPT8 PfRab2 (PFL1500w)
6 YPT32 PfRab11A (PF13_0119)
7 YPT51/Vps21 PfRab5C (PFA0335w)
8 YPT52 PfRab5B (MAL13P1.51)
9 YPT53 PfRab5A (PFB0500c)
10 YPT6 PfRab6 (PF11_0461)
11 YPT7/VAM4/AST4 PfRab7 (PFI0155c)
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Saccharomyces cerevisiae Ypt amino acid sequences were used to query the PlasmoDB ((http://plasmodb.org/plasmo) and the P. falciparum Rab displaying the highest similarity was taken and used to query the SGD data base (http://www.yeastgenome.org). The homology assignment was based on the same Ypt/PfRab pair being identified by such reciprocal BLAST analysis.

Table 2.

Orthology groups for Rab and Ypt proteins in P. falciparum and S. cerevisiae

OrthoGroup PfRab S. cerevisiae P. falciparum PlasmoDB SGD SGD Ypt Ypt
OG4_21598 Rab1A 0 1 PFE0690c
OG4_10503 Rab1B 1 1 PFE0625w YFL038C Ypt1
OG4_11361 Rab2 0 1 PFL1500w
OG4_36791 Rab5A 0 1 PFB0500c
OG4_18709 Rab5B 1 1 MAL13P1.51 YKR014C Ypt52
OG4_10168 Rab5C 2 1 PFA0335w YNL093W YOR089C Ypt53 Ypt51
OG4_10702 Rab6 1 1 PF11_0461 YLR262C Ypt6
OG4_11035 Rab7 1 1 PFI0155c YML001W Ypt7
OG4_10424 Rab11A 2 1 PF13_0119 YGL210W YER031C Ypt32 Ypt31
OG4_21991 Rab11B 0 1 MAL13P1.205
OG4_13079 Rab18 0 1 PF08_0110
OG4_10784 1 YFL005W Sec4
OG4_60929 1 Ypt10
OG4_61055 1 Ypt11
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Orthologue groups were obtained from OrthoMCL DB (http://www.orthomcl.org/cgi-bin/OrthoMclWeb.cgi) and due sequence similarities certain Ypts/PfRabs appear in the same orthology group.

PfRab-interactomes

We show (Figure 1B) the global physical interactome for the PfRab family that will be discussed in detail below (Supplementary Table S2 lists all predicted genetic and physical PfRab-interactants). For comparison with Figure 2, we present the PfRab6, PfRab1B and PfRab11A interactomes (Figure 3). Like Ypt6, parasite Rab6 is involved in many interactions (Figure 3A) and the homology between Ypt6/PfRab6 effectors underscores the conserved role for this Golgi-specific Rab. There is less conservation between the number of effectors for PfRab1B (Figure 3B) and PfRab11A (Figure 3C), but similar to their yeast orthologues they also show interactomes of reduced complexity.

Figure 3. Specific PfRab-interactomes based on all genetic and physical interactions.

Figure 3

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Interactomes of homologues of YPT6, YPT1 and YPT32 that belong to same orthology group. (A) Interactome of PfRab6 and similarly it represents a complex interactome like YPT6. (B) Interactome of PfRab1B and (C) that of PfRab11A. Red represents Rab proteins and pink indicates the interacting protein. Green indicates the proteins that interact with three or more Rab proteins.

PfCK1 is a PfRab5B interactant

Since both P. falciparum and S. cerevisiae have three closely related rab5 genes, we constructed a global Rab5-interactome that illustrates all potential PfRab5-interactions (Figure 4A). There is a myriad of predicted interactants, with a significant number shared with PfRab6 (PF11_0461) (de Castro et al., 1996). Among the specific Rab5-effector proteins, we choose to wet-bench test the physical association predicted with CK1 (PF11_0377, (Barik et al., 1997)) and tested all three His-tagged PfRab5 isoforms for GST-PfCK1 binding in a series of pull-down experiments. In solution and in the presence of 0.4% Triton (see Materials and methods), PfCK1 binds only to PfRab5B (Figure 4B) demonstrating that in spite of their shared sequence identity each PfRab5 isoform can have specific effector proteins.

Figure 4. Interactomes of PfRab5 isoforms based on genetic and physical interactions.

Figure 4

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(A) The interactome of all three PfRab5 isoforms. Red represents Rab proteins and pink indicates the interacting protein. Green indicates the proteins that interact with three or more Rab proteins. (B) Specific physical association between PfRab5B and PfCK1. GST or GST-PfCK1 was incubated in solution with PfRab5A-His or PfRab5B-His or PfRab5C-His recombinant proteins. After washing steps, physical association was revealed by a Western blot with anti-His antibodies. In (B), the amount of input His-tagged Rab protein revealed by anti-His antibodies is given at bottom. In (B), top left shows the amount of GST-only and GST-PfCK1 proteins used as bait and Middle shows that PfRab5B-His binds only to GST-PfCK1 and not to GST, whereas neither PfRab5A nor PfRab5C bind to GST-PfCK1, or GST.

Ypt7 versus PfRab7 interactomes

Given that P. falciparum is a strict intracellular pathogen that resides for long periods within erythrocytes, additional Rabs involved in regulating endocytic processes is of particular interest. In S. cerevisiae one of Ypt7 functions is to regulate homotypic fusion of partner vacuoles, a required step during vacuole inheritance (Haas et al., 1995) and for these reasons we compare in Figure 5 the physical interactomes of Ypt7 and PfRab7. One can immediately see that Ypt7/PfRab7 share a number of effector proteins with Ypt6/PfRab6 implying that these are involved in activities common to both Rabs. Among this group are Hsp82 (YPL240C) and Hsp86 (PF07_0029), and via this interaction PfRab6 and PfRab7 would connect to the Hsp86 network of interactions (Pavithra et al., 2007). With a specific role for PfRab7 in mind the subset of specific effectors are pertinent and here, we note the predicted physical interaction between TPK3 and Ypt7. Our attention was drawn to testing parasite PKA by the recent report that extracellular adenosine signalling in sickle cell red blood cells activates erythrocyte PKA, combined with the observation that ATP signalling via the P2X7 receptor leads to the membrane assembly of actin (Kuehnel et al., 2009a, b; Zhang et al., 2010). Purinergic receptors have been described both at the plasma membrane of P. falciparum-infected erythrocytes (Tanneur et al., 2006) and on the parasite's own plasma membrane (Levano-Garcia et al., 2010).

Figure 5. Comparison of interactomes of YPT7 and PfRab7 based on physical interactions.

Figure 5

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(A) The interactome of YPT7 and (B) that of PfRab7. Red represents Rab proteins and pink indicates the interacting protein. Green indicates the proteins that interact with three or more Rab proteins.

PfPKA-C is a PfRab7-interactant

The Ypt7/PfRab7 interactomes suggest the possibility that in P. falciparum parasites PfPKA-C (Syin et al., 2001) could be recruited to PfRab7+ vesicles/organelles. Moreover, in S. cerevisiae the interactions with the different TPKs are physical and the validity of prediction for P. falciparum can again be tested directly. To this end, we exploited the P. falciparum transgenic line that expresses the PfPKA-C subunit C-terminally tagged with Hemagglutinin (HA) (Leykauf et al., 2010), and assayed for a physical association with PfRab7 both in vitro and in vivo (Figure 6). In a series of in vitro pull-down experiments using GST-tagged PfPKA-C and either His-tagged PfRab7 or His-tagged PfRab5C, we demonstrated that PfPKA-C physically binds PfRab7 in solution and does not bind PfRab5C (Figure 6A).

Figure 6. Physical association between PfRab7 and PfPKA-C in vitro and in vivo.

Figure 6

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(A) PfPKA-C specifically binds in solution to PfRab7, but not to PfRab5C. Top, a Ponceau stain showing the amount of GST and GST-PfPKA-C proteins used as bait. Middle, PfRab7 and not PfRab5C binds to GST-PfPKA-C and neither bind to GST-alone. Bottom, indicates the amount of input His-tagged Rab protein, as estimated with anti-His antibodies. (B) Top, the presence of PfPKA-C-HA in the anti-PfRab7 immunoprecipitate is revealed with an anti-HA antibody. PfPKA-C-HA was not detected when pre-immune anti-PfRab7 serum, or anti-PfRab5C serum were used. Bottom, equivalent amounts of parasite protein extracts were used for precipitations as revealed by reaction with anti-HA antibodies. (C) Association between PfRab7 and PfPKA-C-HA in vivo is shown at a single stack of image acquisition. The distribution of PfRab7 (red) is revealed with a specific anti-peptide PfRab7 antibody and the distribution of PfPKA-C-HA (green) is revealed with anti-HA antibodies. The association between PfRab7 and PfPKA-C-HA is shown in white. The Pearson's coefficient (r) represents the degree of co-localization and the mean value was calculated by analysing all z-stacks from three independent cells. The level of association between Rab7 and PfPKA-C-HA increases as the parasite develops into a multi-nucleated schizont (nuclei shown stained in blue with DAPI). (D) An isosurface 3D-reconstruction with PfRab7 (red) and PfPKA-C-HA (green).

Next, we immunoprecipitated from parasite-infected red blood cells both PfRab5C and PfRab7 with specific antibodies and additionally took pre-immune serum to PfRab7 as a negative control. Equivalent amounts of input protein in the cell extracts were verified with the anti-HA antibody (see Materials and methods for details). The three precipitates were transferred to membrane and probed with an anti-HA antibody and PfPKA-C-HA was only detected associated with PfRab7 in vivo (Figure 6B).

We further verified an in vivo association between PfRab7 and PfPKA-C-HA in parasites by indirect immunofluorescence and one can observe a subset of PfRab7+ vesicles associated with a subset of PfPKA-C-HA+ structures (Figures 6C and 6D). In vivo, the association between PfPKA-C and PfRab7 appears to be dynamic with the degree of co-localization (shown in white) increasing (r values go from 0.204 to 0.418), as the parasite develops from trophozoites (2–3 nuclei stained blue by DAPI) to a multi-nucleated schizont within the red blood cell (Figure 6C and see video Supplementary Figure 3). Non-infected red blood cells failed to stain (bottom panel). An isosurface 3D reconstruction is presented in Figure 6D, together with an enlargement of the region shown boxed. One can clearly see the close juxtaposition of PfRab7 (red) with PfPKA-C (green).

Common effectors shared between PfRab5 and PfRab7

To highlight interactants potentially shared between PfRab5 and PfRab7, we constructed a joint interactome based on both genetic and physical predicted interactions (Figure 7A). The common effectors are colour-coded to distinguish between putative physical (blue) and genetic (purple), and mixed physical (PfRab7) and genetic (PfRab5) interactions are indicated in orange. Among the putative physical effectors shared by PfRab5 and PfRab7 are proteins known to be involved in cycling of Rabs from donor to acceptor compartments. In addition, one can see that PfPKA-C is predicted to bind not only to PfRab7 (see above), but also to a PfRab5 isoform. To identify the PfPKA-C-binding PfRab5 isoform, we again performed pull downs and observed that it binds specifically to PfRab5A and not to PfRab5B, or PfRab5C (Figure 7B).

Figure 7. PfRab5 and PfRab7 shared effectors based on predicted genetic and physical interactions.

Figure 7

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(A) Blue indicates putative physical effectors shared by Rab5 and Rab7 and purple indicates putative genetic effectors shared by Rab5 and Rab7. Out of the shared effectors, the ones that have a putative physical interaction with Rab7 and genetic interaction with Rab5 are indicated in orange. Potential functional pathways have been indicated for Rab5 and Rab7 common effectors. Pink indicates all other putative effectors predicted to specifically interact with only PfRab5, or PfRab7 that are shown in red. (B) Specific physical association between PfRab5A and PfPKA-C. GST-only or GST-PfPKA-C was incubated in solution with PfRab5A-His or PfRab5B-His or PfRab5C-His recombinant proteins. After washing steps, physical association was revealed by a Western blot and the amount of input and bound His-tagged Rab protein revealed by anti-His antibodies. Ponceau staining indicates the amount of GST-only and GST-PfPKA-C proteins used as bait. PfRab5A-His only binds to GST-PfPKA-C and not to GST-alone, whereas neither PfRab5B, nor PfRab5C bind to GST-PfPKA-C, nor GST-alone.

Discussion

The purpose of this comparative Ypt- versus PfRab-interactome study was to identify a set of conserved (between yeast and Plasmodium) proteins that would represent a novel collection of putative PfRab-interactants. Our analysis of the Ypt6 network shows that it displays a remarkable complexity of interactions that clearly underscores the central role that Ypt6 plays in regulating vesicular transport in yeast. It is immediately obvious that like Ypt6, PfRab6 is involved in many interactions highlighting the conserved role for this Golgi-specific GTPase. Furthermore, individual network complexity is compounded by interactions between Ypt6 with Ypt1, Ypt7 and Ypt52/Vps21 that consequently gives rise to interconnecting networks. There is somewhat less conservation between the number of effectors for PfRab1B and PfRab11A, but similar to their yeast orthologues they also show interactomes of reduced complexity. Clearly, there is a correlation between orthology group size for a given Ypt/Rab and the complexity of the corresponding conserved interactome.

We concentrated on predicted physical Rab-interactions, as these could be directly verified experimentally, since testing genetic interactions in Plasmodium parasites is not feasible at this time. To underscore the validity of our approach, we experimentally tested and confirmed three predicted interactions, two for a specific PfRab5 isoform, and one for PfRab7. We provided experimental evidence that supports the prediction that PfCK1 is a PfRab5 effector kinase by demonstrating that PfCK1 binds specifically only to PfRab5B (Figure 4B). The subcellular distribution of PfCK1 has not yet been described (Barik et al., 1997), but in the related apicomplexa Toxoplasma gondii there are two CK1 isoforms with TgCK1-alpha being cytosolic and TgCK1-beta being associated via its C-terminus with the plasma membrane (Donald et al., 2005). Our close inspection of TgCK1-beta identified two potential palmitoylation sites in this region (not shown), but whether PfRab5B is involved in trafficking PfCK1 just to the cytosol, or also to the parasite's plasma membrane remains to be determined. We also demonstrated that PfRab5A binds PfPKA-C and shares this effector kinase with PfRab7. Under the conditions used, the shared sequence identity between the three PfRab5 isoforms did not prevent specific binding of PfCK1 to PfRab5B, or PfPKA-C to PfRab5A.

In T. gondii, TgRab7 has recently been described associated with a novel vacuolar organelle (Miranda et al., 2010; Parussini et al., 2010). Whether the Rab7+ positive vesicular-like structures identified here in P. falciparum correspond to this novel vacuolar organelle remains to be seen. In P. falciparum only a subset of vesicles, the number of which increased as the parasite developed into a multinucleated schizont, were found to interact with PfPKA-C suggesting different, dynamic, sub-populations of PfRab7+ vesicles in vivo. We also observed PfPKA-C+ structures that were negative for PfRab7 and some of these might be associated with PfRab5A+ vesicles, since we failed to observe any interaction with PfRab5B and PfRab5C in vitro or in vivo (Figures 6 and 7).

The genome of S. cerevisiae encodes three PKA-C (TPK) and one PKA-R (BCY1) subunits with BCY1 being structurally and functionally similar to the mammalian RII class of PKA regulators. P. falciparum has only a single regulatory subunit PfPKA-R (Merckx et al., 2008), and a single catalytic subunit PfPKA-C (Syin et al., 2001). TPK1, TPK3 and BCY1 all physically interact with one of the yeast Rab5s (see Supplementary Figure 1F). Consequently, PfPKA-C and PfPKA-R are predicted to physically interact with one of the parasite's PfRab5s (Figures 4 and 7), in addition to PfRab7 (Figure 5). However as stated above, only PfRab5A bound PPfKA-C and whether PfRab5B and/or PfRab5C bind to PfPKA-R will be tested in the future.

The ability of PfRab5A and PfRab7 to bind PfPKA-C suggests that they act somewhat like a PKA-anchor proteins (AKAP), recruiting PKA to early and late endosomes (Beene and Scott, 2007). By using parasite Rabs as pseudo-AKAPs P. falciparum may compensate for the paucity of recognizable AKAPs encoded in its genome, as our bioinformatic searches reveal the presence of only a single AKAP-like protein (PFE0640w) showing similarity to AKAP18 (Gold et al., 2008). Nonetheless, it is interesting to note that mammalian Rab4 and Rab11A have been described to bind D-AKAP2 thereby recruiting PKA to recycling endosomes and altering transferrin receptor turnover (Eggers et al., 2009). It would seem then, that like yeast, malaria parasites have a more direct way of recruiting PKA to endosome/vacuole-like structures. It is of course intriguing as to the potential role PKA might be playing on PfRab5A+ and PfRab7+ structures. An exciting possibility is that PKA might be involved in actin mobilization at the endosomal membrane (Kuehnel et al., 2009a, b) and in such a way regulate the parasite's ability to import nutrient (Elliott et al., 2008; Lazarus et al., 2008) and recycle receptors.

Materials and methods

Rab-interaction data

Saccharomyces cerevisiae was used as the source organism to study PfRab interactors, as its Ypt protein and effectors are characterized and also because it shares the same number of Rab proteins as Plasmodium falciparum (Quevillon et al., 2003). The yeast Ypt/Rab genome sequences as well as the physical and genetic interactors were downloaded from SGD database (http://www.yeastgenome.org). Eight hundred twelve interactions involving 596 proteins were found to be involved in the S. cerevisiae Ypt/Rab-interactome network. All the redundant interactions were removed and the remaining interactions represented as networks using Cytoscape (http://www.cytoscape.org/).

Orthologous protein data for PfRab-interactants

The orthologous protein information was obtained by performing pairwise alignment of the sequences using BLASTp at NCBI (http://blast.ncbi.nlm.nih.gov/Blast.cgi). Orthologue groups were obtained from OrthoMCL DB (http://www.orthomcl.org/cgi-bin/OrthoMclWeb.cgi). BLASTp (E-value≤0.05) was performed by taking S. cerevisiae protein sequences as queries against P. falciparum protein sequences obtained from PlasmoDB (http://plasmodb.org/plasmo) and confirming the hit by reverse BLAST. The first hit obtained was taken as query for the reverse BLAST against non-redundant (nr) protein database with S. cerevisiae as organism. Homologues were obtained for 200 proteins.

PfRab and Rab-interactor homologues

We used BLASTp searches to identify the homologues of S. cerevisiae Ypt proteins and their effectors in P. falciparum. For those proteins where reverse BLASTp failed to identify a clear homologue due to the best hit being already assigned, the second best hit was taken as the homologue. Due to a high degree of similarity between Ypt10 and Ypt11, multiple sequence alignments (ClustalW) (http://www.ebi.ac.uk/Tools/clustalw2/index.html) were applied together with PfRab11B and PfRab18.

Constructs for expression of recombinant proteins

To make C-terminal PfRab7-His, PfRab5A-His, PfRab5B-His and PfRab5C-His constructs for expression in Escherichia coli, the CDS of Pfrab7 (PFI0155c), Pfrab5a (PFB0500c), Pfrab5b (MAL13P1.51) and Pfrab5c (PFA0335w) were PCR amplified from cDNA of P. falciparum (3D7) and cloned into BamHI/XhoI site of the E. coli expression vector pET-21d (Novagen, EMD4Biosciences). The sequences of all expression constructs were verified. To make the N-terminal GST-PfPKA-C construct, a codon optimized synthetic Pfpka-c gene (PFI1685w) was custom made (Eurobio) in order to improve recombinant protein levels when expressed in E. coli.

Protein expression in E. coli

All constructs were transformed into BL21-CodonPlus (DE3)− RIL strain (Stratagene). LB media contained 34 μg/ml chloramphenicol and 100 μg/ml ampicillin and cells were grown at 37°C to an absorbance at 600 nm. Proteins expression were induced by adding 0.2 mM IPTG for PfRab5A-His, PfRab5B-His, PfRab5C-His and PfRab7-His and 1mM IPTG for GST-PfPKA-C, GST-PfCK1 and GST-alone, and the cultures were incubated overnight at 20°C. Cells were harvested by centrifugation at 5000× g for 20 min. Harvested cells were resuspended in urea 6 M buffer supplemented with protease inhibitor cocktail (Roche) for PfRab proteins and in PBS 1X, 1% Triton 100X, 1 mM EDTA buffer supplemented with protease inhibitor cocktail (Roche) for GST-PfPKA-C, GST-PfCK1 and GST-alone, then stored at −80°C. His-tagged proteins were purified on Ni-NTA agarose (Qiagen) in the case of PfRab5A, PfRab5B, PfRab5C and PfRab7, and on Glutathione Sepharose TM 4B beads (GE Healthcare) in case of GST-tagged proteins.

GST pull downs and western blotting

For GST pull-down experiments, Glutathione Sepharose TM 4B (GE Healthcare) coupled to GST or GST-PfPKA-C or GST-PfCK1 (approximately 2–3 μg of proteins coupled) were incubated with 10 μg of PfRab7-His or PfRab5A-His or PfRab5B-His or PfRab5C-His recombinant proteins overnight at 4°C. These samples were washed four times in PBS (0.4% Triton X-100) and the proteins were eluted by boiling in Laemmli sample buffer and separated by electrophoresis in a 15% SDS-polyacrylamide gel and transferred into nitrocellulose membrane. The blots were first incubated with the mouse monoclonal anti-histidine (1:1000, Covance) and then incubated with the anti-mouse peroxidase-conjugated secondary antibody (1:5000, Sigma Aldrich). Immunoblots were developed by chemiluminescence using ECL (Pierce).

Cells lines

The P. falciparum 3D7 transgenic line expressing PfPKA-C-HA (Leykauf et al., 2010) was cultured in human 0+ erythrocytes and positive selection of PfPKA-C-HA expressing parasites was achieved using 10nM WR99210, an anti-folate that selects for the presence of the human dhfr gene (de Koning-Ward et al., 2000).

PfRab-specific antibodies

To generate the PfRab5C-specific antibody, PfRab5C-His protein was purified (see section: Protein expression in E. coli section) and used to immunize two rabbits (Eurogentec). To generate specific PfRab7 antibodies, two peptides (FALNNQSEQKMYKSR and FLIQASPKDPENFPF) were synthesized and used to immunize two rats (Eurogentec).

Co-immunoprecipitation and western blotting

Thirty millilitre of parasite culture at 10% of parasitemia (mostly schizonts) expressing PfPKA-C-HA were saponin lysed. The resulting parasite pellet was washed in ice-cold PBS and lysed in ten volumes of ice-cold RIPA (Euromedex) buffer complemented with protease inhibitor cocktail (Roche). The membrane fraction was separated from soluble proteins by centrifugation at 13000× g for 30 min. Protein concentration was evaluated in the resulting supernatant by the Bradford method (Biorad). Five hundred microgram of protein extract was incubated for two h at 4°C with the rat pre-immune serum, anti-PfRab7 antibodies (a mix of both anti-peptide antibodies), or with rabbit anti-PfRab5C antibody, all at 50 μg/ml (Bradford estimation). Thirty microliter of protein-G sepharose beads (GE healthcare) was added to precipitate the complexes and incubated overnight at 4°C. Protein-G sepharose beads were washed four times with ten volumes of cold RIPA buffer. Proteins were eluted by boiling in Laemmli sample buffer and separated by electrophoresis through a 15% SDS-polyacrylamide gel and transferred into nitrocellulose membrane. The blot was incubated with the anti-HA rat antibody (1:1000, Roche) and then incubated with the peroxidase-conjugated anti-rat secondary antibody (1:5000). Immunoblots were developed by chemiluminescence using ECL (Pierce).

Immunofluorescence

Smears of red blood cells infected with P. falciparum expressing PfPKA-C-HA were fixed using 100% cold methanol for 5 min. Cells were washed with PBS, and were permeabilized with 0.1% Triton 100X in PBS for 5 min. After washing with PBS, slides were blocked in 3% BSA PBS for 1 h at room temperature. The slides were incubated successively for 1 h with first antibodies: rat anti-PfRab7 (1:300) and mouse anti-HA (1:200, Sigma Aldrich). The slides were washed four times and incubated with AlexaFluor 594 anti-rat IgG antibodies (1:3000, Molecular Probes Probes), with AlexaFluor 488 anti-mouse IgG antibodies (1:3000, Molecular Probes Probes), with DAPI (1 μg/ml) and then washed and mounted (Dako). Cells were examined under a microscope (Leica DMI 6000, X 100 objective, NA 1.4 oil) with a cooled charge-coupled device camera (Micromax). Images were acquired with z stacks with MetaMorph (Universal Imaging) and de-convoluted with Huygens (SVI). Images were analysed and processed with ImageJ (NIH) and Photoshop (Adobe Systems Inc.). For the merge, the ImageJ co-localization plug-in was used. Pearson's coefficient of co-localization was attributed with the ImageJ JACoP plug-in (Bolte and Cordelieres, 2006). Using immunofluorescence images corresponding to the different z stacks (of the cell in upper middle panel of Figure 6C), a volumic (Supplementary Figure 3) and isosurface (Figure 6D) 3D-modelizations were performed with Imaris (Bitplane).

Author contribution

Syama Chandran and Vrushali Dandavate performed the BLAST analysis and constructed the yeast and parasite Rab interactomes. Fathia Ben Rached, Carinne Ndjembo-Ezougou and Hélène Yera did all the wet-bench testing of predictions. Hana Talabani provided PfPKA-C recombinant protein and Pierre Bourdoncle the image analyses. Markus Meissner advised on orthology group comparisons and Utpal Tatu and Gordon Langsley designed the study with Gordon Langsley writing the paper.

Acknowledgments

We thank Tim Wolf Gilberger for gift of the PfPKA-C-HA P. falciparum transgenic line. We thank Dominique Dorin-Semblat and Christian Doerig for gift of the PfCK1-GST expression plasmid and Anais Merckx for help with His-tagged Rab expression. We thank Jacobus Pharmaceuticals (Princeton, USA) for providing WR99210 free of charge. We acknowledge the Institute Cochin's Imaging Facility and both the SGD (http://www.yeastgenome.org) and PlasmoDB (http://www.plasmodb.org) databases for making data freely available. FBR is a recipient of a PhD fellowship from the Fondation pour la Recherche Médicale and CNE a PhD fellowship from the Agence Universitaire de la Francophonie. We acknowledge financial support from the European Union MALSIG grant to MM, UT, and GL and GL also recognizes INSERM and CNRS infrastructure support.

Supplementary material

Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors.

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