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. 1977 May;4(5):1273–1289. doi: 10.1093/nar/4.5.1273

A restriction endonuclease cleavage map of mouse mitochondrial DNA.

K H Moore, P H Johnson, S E Chandler, L I Grossman
PMCID: PMC343755  PMID: 331253

Abstract

A restriction endonuclease cleavage map is presented for mouse mitochondrial DNA. This map was constructed by electron microscopic measurements on partial digests containing fixed D-loops, and by electrophoretic analysis of partial and complete single enzyme digests, and of double digests. No map differences were detected between mitochondrial DNA from cultured LA9 cells and an inbred mouse line for the six endonucleases used. Three cleavage sites recognized by HpaI, five sites recognized by HincII, two sites recognized PstI and four sites recognized by BamI were located with respect to the origin of replication and the EcoRI and HinIII sites previously determined by others. No cleavages were produced by KpnI or SalI. The migration of linear DNA with a molecular weight greater than 1 X 10(6) was not a linear function of log molecular weight in 1% agarose gels run at 6.6 volts/cm.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berk A. J., Clayton D. A. Mechanism of mitochondrial DNA replication in mouse L-cells: asynchronous replication of strands, segregation of circular daughter molecules, aspects of topology and turnover of an initiation sequence. J Mol Biol. 1974 Jul 15;86(4):801–824. doi: 10.1016/0022-2836(74)90355-6. [DOI] [PubMed] [Google Scholar]
  2. Berk A. J., Clayton D. A. Mechanism of mitochondrial DNA replication in mouse L-cells: topology of circular daughter molecules and dynamics of catenated oligomer formation. J Mol Biol. 1976 Jan 5;100(1):85–92. doi: 10.1016/s0022-2836(76)80036-8. [DOI] [PubMed] [Google Scholar]
  3. Blin N., von Gabain A., Bujard H. Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. FEBS Lett. 1975 Apr 15;53(1):84–86. doi: 10.1016/0014-5793(75)80688-0. [DOI] [PubMed] [Google Scholar]
  4. Brown W. M., Vinograd J. Restriction endonuclease cleavage maps of animal mitochondrial DNAs. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4617–4621. doi: 10.1073/pnas.71.11.4617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown W. M., Watson R. M., Vinograd J., Tait K. M., Boyer H. W., Goodman H. M. The structures and fidelity of replication of mouse mitochondrial DNA-pSC 101 EcoRI recombinant plasmids grown in E. coli K12. Cell. 1976 Apr;7(4):517–530. doi: 10.1016/0092-8674(76)90202-6. [DOI] [PubMed] [Google Scholar]
  6. Clayton D. A., Smith C. A. Complex mitochondrial DNA. Int Rev Exp Pathol. 1975;14:1–67. [PubMed] [Google Scholar]
  7. Clayton D. A., Smith C. A., Jordan J. M., Teplitz M., Vinograd J. Occurrence of complex mitochondrial DNA in normal tissues. Nature. 1968 Dec 7;220(5171):976–979. doi: 10.1038/220976a0. [DOI] [PubMed] [Google Scholar]
  8. Clayton D. A., Vinograd J. Circular dimer and catenate forms of mitochondrial DNA in human leukaemic leucocytes. Nature. 1967 Nov 18;216(5116):652–657. doi: 10.1038/216652a0. [DOI] [PubMed] [Google Scholar]
  9. Clayton D. A., Vinograd J. Complex mitochondrial DNA in leukemic and normal human myeloid cells. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1077–1084. doi: 10.1073/pnas.62.4.1077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fauron C. M., Wolstenholme D. R. Structural heterogeneity of mitochondrial DNA molecules within the genus Drosophila. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3623–3627. doi: 10.1073/pnas.73.10.3623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Flory P. J., Jr, Vinograd J. 5-bromodeoxyuridine labeling of monomeric and catenated circular mitochondrial DNA in HeLa cells. J Mol Biol. 1973 Feb 25;74(2):81–94. doi: 10.1016/0022-2836(73)90100-9. [DOI] [PubMed] [Google Scholar]
  12. Hayward G. S., Smith M. G. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972 Feb 14;63(3):383–395. doi: 10.1016/0022-2836(72)90435-4. [DOI] [PubMed] [Google Scholar]
  13. Hsu M. T., Kung H. J., Davidson N. An electron microscope study of Sindbis virus RNA. Cold Spring Harb Symp Quant Biol. 1974;38:943–950. doi: 10.1101/sqb.1974.038.01.096. [DOI] [PubMed] [Google Scholar]
  14. Hudson B., Vinograd J. Sedimentation velocity properties of complex mitochondrial DNA. Nature. 1969 Jan 25;221(5178):332–337. doi: 10.1038/221332a0. [DOI] [PubMed] [Google Scholar]
  15. Johnson P. H., Lee A. S., Sinsheimer R. L. Production of specific fragments of phi X174 replicative form DNA by a restriction enzyme from Haemophilus parainfluenzae, endonuclease HP. J Virol. 1973 Apr;11(4):596–599. doi: 10.1128/jvi.11.4.596-599.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Johnson P. H., Sinsheimer R. L. Structure of an intermediate in the replication of bacteriophage phiX174 deoxyribonucleic acid: the initiation site for DNA replication. J Mol Biol. 1974 Feb 15;83(1):47–61. doi: 10.1016/0022-2836(74)90423-9. [DOI] [PubMed] [Google Scholar]
  17. Kasamatsu H., Grossman L. I., Robberson D. L., Watson R., Vinograd J. The replication and structure of mitochondrial DNA in animal cells. Cold Spring Harb Symp Quant Biol. 1974;38:281–288. doi: 10.1101/sqb.1974.038.01.031. [DOI] [PubMed] [Google Scholar]
  18. Kasamatsu H., Robberson D. L., Vinograd J. A novel closed-circular mitochondrial DNA with properties of a replicating intermediate. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2252–2257. doi: 10.1073/pnas.68.9.2252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kasamatsu H., Vinograd J. Replication of circular DNA in eukaryotic cells. Annu Rev Biochem. 1974;43(0):695–719. doi: 10.1146/annurev.bi.43.070174.003403. [DOI] [PubMed] [Google Scholar]
  20. Kasamatsu H., Vinograd J. Unidirectionality of replication in mouse mitochondrial DNA. Nat New Biol. 1973 Jan 24;241(108):103–105. doi: 10.1038/newbio241103a0. [DOI] [PubMed] [Google Scholar]
  21. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  22. Neal M. W., Florini J. R. A rapid method for desalting small volumes of solution. Anal Biochem. 1973 Sep;55(1):328–330. doi: 10.1016/0003-2697(73)90325-4. [DOI] [PubMed] [Google Scholar]
  23. Pikó L., Matsumoto L. Complex forms and replicative intermediates of mitochondrial DNA in tissues from adult and senescent mice. Nucleic Acids Res. 1977;4(5):1301–1314. doi: 10.1093/nar/4.5.1301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. RICHARDSON C. C., LEHMAN I. R., KORNBERG A. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY. J Biol Chem. 1964 Jan;239:251–258. [PubMed] [Google Scholar]
  25. RICHARDSON C. C., SCHILDKRAUT C. L., APOSHIAN H. V., KORNBERG A. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. J Biol Chem. 1964 Jan;239:222–232. [PubMed] [Google Scholar]
  26. Robberson D. L., Clayton D. A., Morrow J. F. Cleavage of replicating forms of mitochondrial DNA by EcoRI endonuclease. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4447–4451. doi: 10.1073/pnas.71.11.4447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Robberson D. L., Clayton D. A. Pulse-labeled components in the replication of mitochondrial deoxyribonucleic acid. J Biol Chem. 1973 Jun 25;248(12):4512–4514. [PubMed] [Google Scholar]
  28. Robberson D. L., Clayton D. A. Replication of mitochondrial DNA in mouse L cells and their thymidine kinase - derivatives: displacement replication on a covalently-closed circular template. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3810–3814. doi: 10.1073/pnas.69.12.3810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Robberson D. L., Kasamatsu H., Vinograd J. Replication of mitochondrial DNA. Circular replicative intermediates in mouse L cells. Proc Natl Acad Sci U S A. 1972 Mar;69(3):737–741. doi: 10.1073/pnas.69.3.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Smith C. A., Jordan J. M., Vinograd J. In vivo effects of intercalating drugs on the superhelix density of mitochondrial DNA isolated from human and mouse cells in culture. J Mol Biol. 1971 Jul 28;59(2):255–272. doi: 10.1016/0022-2836(71)90050-7. [DOI] [PubMed] [Google Scholar]
  31. Smith C. A., Vinograd J. Complex mitochondrial DNA in human tumors. Cancer Res. 1973 May;33(5):1065–1070. [PubMed] [Google Scholar]
  32. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  33. Wilson G. A., Young F. E. Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H. J Mol Biol. 1975 Sep 5;97(1):123–125. doi: 10.1016/s0022-2836(75)80028-3. [DOI] [PubMed] [Google Scholar]
  34. Wolstenholme D. R., Koike K., Cochran-Fouts P. Replication of mitochondrial DNA: replicative forms of molecules from rat tissues and evidence for discontinuous replication. Cold Spring Harb Symp Quant Biol. 1974;38:267–280. doi: 10.1101/sqb.1974.038.01.030. [DOI] [PubMed] [Google Scholar]
  35. Zeiger R. S., Salomon R., Dingman C. W., Peacock A. C. Role of base composition in the electrophoresis of microbial and crab DNA in polyacrylamide gels. Nat New Biol. 1972 Jul 19;238(81):65–69. doi: 10.1038/newbio238065a0. [DOI] [PubMed] [Google Scholar]

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