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. 1977 May;4(5):1349–1359. doi: 10.1093/nar/4.5.1349

Ethidium analogues with improved resolution in the dye-buoyant density procedure.

B Hudson, J H Dawson, R Desiderio, C W Mosher
PMCID: PMC343760  PMID: 896463

Abstract

Analogues of ethidium chloride with large quaternary side chains have been synthesized and evaluated for improved resolution in the dye-buoyant density system for the separation of open and closed circular DNA. These compounds are similar to propidium which differs from ethidium by the replacement of an ethyl group by a methyldiethylaminopropyl group. The new analogues contain a triethylamino group attached to a methylene chain with 3, 5 or 7 carbons. With PM2 DNA the observed separation for propidium is 1.97 times that of ethidium and the new dyes show separations relative to ethidium of 2.27, 2.66 and 2.77. A correlation is established between the mass of the dye component and the observed separation which is retionalized on the basis of the four component thermodynamics describing this system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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