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. Author manuscript; available in PMC: 2013 Mar 6.
Published in final edited form as: Nature. 2012 Sep 6;489(7414):115–120. doi: 10.1038/nature11376

Figure 2. Molecular interfaces in the RNF4 RING–UbcH5a~Ub complex.

Figure 2

a, Detail of the interaction between ubiquitin (orange) and the α2 helix of UbcH5a (green). b, Detail of the interaction interface between ubiquitin (orange) and UbcH5a (green) in the E2 active site groove. The side chain of K85 in UbcH5a that forms the isopeptide bond with ubiquitin is coloured violet. c, The hydrophobic cluster at the centre of the ubiquitin (orange), UbcH5a (green), RING (cyan) heterotrimer. d, Stacking interaction between the main chain of ubiquitin (orange) in one heterotrimer and Y193 of the RING (blue) from the other heterotrimer.