Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1974 Sep;1(9):1143–1151. doi: 10.1093/nar/1.9.1143

The fractionation of calf thymus DNA on histone KAP - Sepharose 4B columns

H Panusz 1, J Bartkowiak 1, A Krzywiec 1, M Joustra 1, A Płucienniczak 1
PMCID: PMC344336  PMID: 4476912

Abstract

It was found that fractionation of calf thymus DNA on homologous histtone KAP covalently bound to CNBr activated Sepharose 4B depends on the molecular weight of DNA. The maximum of elution of high molecular DNA (m. wt. above 5 × 106) was observed at 0.56 M NaCl and that of degraded DNA (m. wt. 0.8 × 106) at 0.52 M NaCl. Significant differences in melting temperatures and melting intervals were observed among fractions obtained from low molecular DNA as a result of enrichment of some fractions in satellite DNAs. These differences were very small in DNA of m. wt. above 5 × 106. The results are discussed in terms of specific areas which may exist on calf thymus DNA molecules playing the role of loci, where lysine-rich histone KAP is preferentially bound.

Full text

PDF
1143

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ayad S. R., Parker M. C. Interactions between histone and DNA from calf and human tissues using activated sepharose column chromatography. J Chromatogr. 1973 Aug 1;82(2):343–348. doi: 10.1016/s0021-9673(01)91840-0. [DOI] [PubMed] [Google Scholar]
  2. Bradbury E. M., Carpenter B. G., Rattle H. W. Magnetic resonance studies of deoxyribonucleoprotein. Nature. 1973 Jan 12;241(5385):123–126. doi: 10.1038/241123a0. [DOI] [PubMed] [Google Scholar]
  3. Clark R. J., Felsenfeld G. Association of arginine-rich histones with G-C-rich regions of DNA in chromatin. Nat New Biol. 1972 Dec 20;240(103):226–229. doi: 10.1038/newbio240226a0. [DOI] [PubMed] [Google Scholar]
  4. Fambrough D., Bonner J. Selective dissociation of pea bud nucleohistone. Biochim Biophys Acta. 1968 Apr 9;154(3):601–602. doi: 10.1016/0005-2795(68)90025-1. [DOI] [PubMed] [Google Scholar]
  5. Ilyin Y. V., Varshavsky A. Y., Mickelsaar U. N., Georgiev G. P. Studies on deoxyribonucleoprotein structure. Redistribution of proteins in mixtures of deoxyribonucleoproteins, DNA and RNA. Eur J Biochem. 1971 Sep 24;22(2):235–245. doi: 10.1111/j.1432-1033.1971.tb01537.x. [DOI] [PubMed] [Google Scholar]
  6. Johns E. W. Studies on histones. 7. Preparative methods for histone fractions from calf thymus. Biochem J. 1964 Jul;92(1):55–59. doi: 10.1042/bj0920055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Plucienniczak A., Bartkowiak J., Krzywiec A., Panusz H. Fractionation of calf thymus DNA based on its interaction with homologeous f-1 histone. Melting curves of the obtained fractions. Biochem Biophys Res Commun. 1974 Feb 4;56(3):799–806. doi: 10.1016/0006-291x(74)90676-7. [DOI] [PubMed] [Google Scholar]
  8. Varshavsky A. J., Georgiev G. P. Clustered arrangement of histones F2al and F3 along DNA in chromosomal deoxyribonucleoproteins. Biochim Biophys Acta. 1972 Nov 9;281(4):669–674. doi: 10.1016/0005-2787(72)90166-9. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES