Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1974 Oct;1(10):1287–1304. doi: 10.1093/nar/1.10.1287

Nucleotide sequence determination of bacteriophage T4 glycine transfer ribonucleic acid

Stephen Stahl 1,*, Gary V Paddock 1, John Abelson 1
PMCID: PMC344351  PMID: 10793690

Abstract

The nucleotide sequence of a T4 tRNA with an anticodon for glycine has been determined using 32P-labeled material from T4-infected cultures of Escherichia coli. The sequence is: pGCGGAUAUCGUAUAAUGmGDAUUACCUCAGACUUCCAAψCUGAUGAUGUGAGTψCGAUUCUCAUUAUCCGCUCCA-OH. The 74 nucleotide sequence can be arranged in the classic cloverleaf pattern for tRNAs. The anticodon of T4 tRNAGly is UCC with a possible modification of the U. The tRNA molecule would thus be expected to recognize the glycine codons GGG and GGA. Comparative analysis of tRNAsGly from T2 and T6 indicate that their sequences are identical with that from T4.

Full text

PDF
1287

Images in this article

1288AImage
on p.1288A
1288BImage
on p.1288B
1292AImage
on p.1292A

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adesnik M., Levinthal C. RNA metabolism in T4-infected Escherichia coli. J Mol Biol. 1970 Mar 14;48(2):187–208. doi: 10.1016/0022-2836(70)90156-7. [DOI] [PubMed] [Google Scholar]
  2. Brownlee G. G., Sanger F. Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coli. J Mol Biol. 1967 Feb 14;23(3):337–353. doi: 10.1016/s0022-2836(67)80109-8. [DOI] [PubMed] [Google Scholar]
  3. Carbon J., Curry J. B. Genetically and chemically derived missense suppressor transfer RNA's with altered enzymic aminoacylation rates. J Mol Biol. 1968 Dec 14;38(2):201–216. doi: 10.1016/0022-2836(68)90406-3. [DOI] [PubMed] [Google Scholar]
  4. Daniel V., Sarid S., Littauer U. Z. Bacteriophage induced transfer RNA in Escherichia coli. New transfer RNA molecules are synthesized on the bacteriophage genome. Science. 1970 Mar 27;167(3926):1682–1688. doi: 10.1126/science.167.3926.1682. [DOI] [PubMed] [Google Scholar]
  5. Goodman H. M., Abelson J. N., Landy A., Zadrazil S., Smith J. D. The nucleotide sequences of tyrosine transfer RNAs of Escherichia coli. Eur J Biochem. 1970 Apr;13(3):461–483. doi: 10.1111/j.1432-1033.1970.tb00950.x. [DOI] [PubMed] [Google Scholar]
  6. Hill C. W., Combriato G., Steinhart W., Riddle D. L., Carbon J. The nucleotide sequence of the GGG-specific glycine transfer ribonucleic acid of Escherichia coli and of Salmonella typhimurium. J Biol Chem. 1973 Jun 25;248(12):4252–4262. [PubMed] [Google Scholar]
  7. Hsu W. T., Foft J. W., Weiss S. B. Effect of bacteriophage infection on the sulfur-labeling of sRNA. Proc Natl Acad Sci U S A. 1967 Nov;58(5):2028–2035. doi: 10.1073/pnas.58.5.2028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McClain W. H., Guthrie C., Barrell B. G. Eight transfer RNAs induced by infection of Escherichia coli with bacteriophage T4. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3703–3707. doi: 10.1073/pnas.69.12.3703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pinkerton T. C., Paddock G., Abelson J. Bacteriophage T4 tRNA Leu . Nat New Biol. 1972 Nov 15;240(98):88–90. doi: 10.1038/newbio240088a0. [DOI] [PubMed] [Google Scholar]
  10. Pinkerton T. C., Paddock G., Abelson J. Nucleotide sequence determination of bacteriophage T4 leucine transfer ribonucleic acid. J Biol Chem. 1973 Sep 25;248(18):6348–6365. [PubMed] [Google Scholar]
  11. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  12. Scherberg N. H., Weiss S. B. Detection of bacteriophage T4- and T5-coded transfer RNAs. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1164–1171. doi: 10.1073/pnas.67.3.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Squires C., Carbon J. Normal and mutant glycine transfer RNAs. Nat New Biol. 1971 Oct 27;233(43):274–277. doi: 10.1038/newbio233274a0. [DOI] [PubMed] [Google Scholar]
  14. Stahl S., Paddock G., Abelson J. T4 bacteriophage tRNAGly. Biochem Biophys Res Commun. 1973 Sep 18;54(2):567–569. doi: 10.1016/0006-291x(73)91460-5. [DOI] [PubMed] [Google Scholar]
  15. Wilson J. H., Kim J. S., Abelson J. N. Bacteriophage T4 transfer RNA. 3. Clustering of the genes for the T4 transfer RNA's. J Mol Biol. 1972 Nov 28;71(3):547–556. doi: 10.1016/s0022-2836(72)80022-6. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES