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. 1974 Oct;1(10):1329–1341. doi: 10.1093/nar/1.10.1329

Tritium sequence analysis of oligoribonucleotides: a combination of post-labeling and thin-layer chromatographic techniques for the analysis of partial snake venom phosphodiesterase digests

Muthumalayan Sivarajan 1, Ramesh C Gupta 1, Li-Li SY Chia 1, Erika Randerath 1, Kurt Randerath 1
PMCID: PMC344354  PMID: 10793693

Abstract

A tritium derivative method for sequence analysis of polyribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide-3′ dialdehydes generated by controlled snake venom phosphodiesterase/alkaline phosphomonoesterase digestion and periodate treatment of time point aliquots of the incubation mixture. Radioactive oligonucleotide derivatives are resolved according to chain length by PEI-cellulose1 anion-exchange TLC and their 3′-termini identified by techniques described in the preceding paper of this series2. The present tritium derivative method is compared with the one described previously2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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