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. 1975 Aug;2(8):1381–1390. doi: 10.1093/nar/2.8.1381

DNA replication in Physarum polycephalum: characterization of replication products in vivo.

S Funderud, F Haugli
PMCID: PMC344389  PMID: 1237130

Abstract

Synchronous plasmodia of Physarum polycephalum in DNA synthesis were pulse-labelled with [oH]- thymidine for time periods of 15 seconds up to 9 minutes, or given a 30 seconds pulse followed by chase periods of 9 minutes up to 6 hours. Sedimentation analysis in alkaline sucrose gradients revealed at least five species of single stranded DNA14 molecules in the pulse experiments. Co-sedimentation of [14C]-labelled phage-DNA gave relative S-values of 5-7, 13-15, 23-25, 30 and 33-35 for these DNA molecules, all of which can be chased into DNA of higher molecular weight.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURGI E., HERSHEY A. D. Sedimentation rate as a measure of molecular weight of DNA. Biophys J. 1963 Jul;3:309–321. doi: 10.1016/s0006-3495(63)86823-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braun R., Wili H. Time sequence of DNA replication in Physarum. Biochim Biophys Acta. 1969 Jan 21;174(1):246–252. doi: 10.1016/0005-2787(69)90248-2. [DOI] [PubMed] [Google Scholar]
  3. Brewer E. N. DNA replication in Physarum polycephalum. J Mol Biol. 1972 Jul 28;68(3):401–412. doi: 10.1016/0022-2836(72)90094-0. [DOI] [PubMed] [Google Scholar]
  4. Brewer E. N., Evans T. E., Evans H. H. Studies on the mechanism of DNA replication in Physarum polycephalum. J Mol Biol. 1974 Dec 5;90(2):335–342. doi: 10.1016/0022-2836(74)90377-5. [DOI] [PubMed] [Google Scholar]
  5. Gautschi J. R., Clarkson J. M. Discontinuous DNA replication in mouse P-815 cells. Eur J Biochem. 1975 Jan 2;50(2):403–412. doi: 10.1111/j.1432-1033.1975.tb09816.x. [DOI] [PubMed] [Google Scholar]
  6. Hershey H. V., Taylor J. H. DNA replication in eucaryotic nuclei. Evidence suggesting a specific model of replication. Exp Cell Res. 1974 Mar 30;85(1):79–88. doi: 10.1016/0014-4827(74)90215-8. [DOI] [PubMed] [Google Scholar]
  7. Huberman J. A., Riggs A. D. On the mechanism of DNA replication in mammalian chromosomes. J Mol Biol. 1968 Mar 14;32(2):327–341. doi: 10.1016/0022-2836(68)90013-2. [DOI] [PubMed] [Google Scholar]
  8. Lindqvist B. H. Vegetative DNA of temperate coliphage P2. Mol Gen Genet. 1971;110(2):178–196. doi: 10.1007/BF00332647. [DOI] [PubMed] [Google Scholar]
  9. Mandel M. Infectivity of phage P2 DNA in presence of helper phage. Mol Gen Genet. 1967;99(1):88–96. doi: 10.1007/BF00306461. [DOI] [PubMed] [Google Scholar]
  10. Mohberg J., Babcock K. L., Haugli F. B., Rusch H. P. Nuclear DNA content and chromosome numbers in the myxomycete Physarum polycephalum. Dev Biol. 1973 Oct;34(2):228–245. doi: 10.1016/0012-1606(73)90352-7. [DOI] [PubMed] [Google Scholar]
  11. Olivera B. M., Bonhoeffer F. Discontinuous DNA replication in vitro. I. Two distinct size classes of intermediates. Nat New Biol. 1972 Dec 20;240(103):233–235. doi: 10.1038/newbio240233a0. [DOI] [PubMed] [Google Scholar]
  12. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]

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