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. 1975 Aug;2(8):1391–1400. doi: 10.1093/nar/2.8.1391

Specificity of deoxyribonucleic acid transmethylase induced by bacteriophage T2. I. Nucleotide sequences isolated from tmicrococcus luteus DNA methylated in vitro.

H van Ormondt, J Gorter, K J Havelaar, A de Waard
PMCID: PMC344390  PMID: 1101223

Abstract

(Deoxyribonucleic acid from Micrococcus luteus was methylated in vitro in the presence of S-adenosyl-(14C methyl)methionine with a DNA methyltransferase purified from extracts of te. coli infected with bacteriophage T2. The labelled DNA was degraded by enzymatic and specific chemical methods and the resulting short oligonucleotides were separated and characterized. tthe analytical data permit the conclusion that the tdna transmethylase reacts specifically with N-G-A-T-C-N sequences in which it converts adenine to a 6-methyl-aminopurine residue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brockes J. P., Brown P. R., Murray K. Nucleotide sequences at the sites of action of the deoxyribonucleic acid modification enzyme of bacteriophage P1. J Mol Biol. 1974 Sep 15;88(2):437–443. doi: 10.1016/0022-2836(74)90493-8. [DOI] [PubMed] [Google Scholar]
  2. Falaschi A., Kornberg A. A lipopolysaccharide inhibitor of a DNA methyl transferase. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1713–1720. doi: 10.1073/pnas.54.6.1713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GOLD M., HAUSMANN R., MAITRA U., HURWITZ J. THE ENZYMATIC METHYLATION OF RNA AND DNA. 8. EFFECTS OF BACTERIOPHAGE INFECTION ON THE ACTIVITY OF THE METHYLATING ENZYMES. Proc Natl Acad Sci U S A. 1964 Aug;52:292–297. doi: 10.1073/pnas.52.2.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GOLD M., HURWITZ J. THE ENZYMATIC METHYLATION OF RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID. V. PURIFICATION AND PROPERTIES OF THE DEOXYRIBONUCLEIC ACID-METHYLATING ACTIVITY OF ESCHERICHIA COLI. J Biol Chem. 1964 Nov;239:3858–3865. [PubMed] [Google Scholar]
  5. Hausmann R., Gold M. The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. IX. Deoxyribonucleic acid methylase in bacteriophage-infected Escherichia coli. J Biol Chem. 1966 May 10;241(9):1985–1994. [PubMed] [Google Scholar]
  6. Hehlmann R., Hattman S. Mutants of bacteriophage T2 gt with altered DNA methylase activity. J Mol Biol. 1972 Jun 28;67(3):351–360. doi: 10.1016/0022-2836(72)90455-x. [DOI] [PubMed] [Google Scholar]
  7. Murray K. Nucleotide 'maps' of digests of deoxyribonucleic acid. Biochem J. 1970 Aug;118(5):831–841. doi: 10.1042/bj1180831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Revel H. R., Hattman S. M. Mutants of T2gt with altered DNA methylase activity: relation to restriction by prophage P1. Virology. 1971 Aug;45(2):484–495. doi: 10.1016/0042-6822(71)90348-5. [DOI] [PubMed] [Google Scholar]
  9. van Ormondt H., Lautenberger J. A., Linn S., de Waard A. Methylated oligonucleotides derived from bacteriophage fd RF-DNA modified in vitro by E. coli B modification methylase. FEBS Lett. 1973 Jul 1;33(2):177–180. doi: 10.1016/0014-5793(73)80186-3. [DOI] [PubMed] [Google Scholar]

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