Fig. 3.
Dynamic p4EBP expression at mid and late third instar stages. p4EBP expression is white in column 3 and blue in column 4. (A-B‴,G-G‴) In WT (dome-gal4, UAS-2xEGFP), p4EBP is expressed at low levels and in scattered p4EBPhigh cells. (C-F‴,H-I‴) Downregulation of Tsc2 (dome>Tsc2RNAi) or Pten (dome>PtenRNAi) in progenitors expands a population of p4EBPhigh cells along the MZ/CZ boundary at mL3 (C-F‴). Arrows in D-D‴ and F-F‴ point to PXN+/p4EBPhigh cells. By lL3, high p4EBP expression expands throughout medial regions upon Tsc2 downregulation (H-H‴), but is highest upon Pten downregulation in differentiating cells at the LG periphery (I-I‴), including within buds of CZ tissue (arrow, I″). Scale bars: 20 μm; in A for A-A‴,C-C‴,E-E‴; in B for B-B‴,D-D‴,F-F‴; in G for G-I‴.