Figure 4A Following LPS stimulation (10 μg/mL LPS) chow significantly increased tissue culture sPLA2 activity compared with baseline (LPS0) and PN was increased but failed to reach significance due to variation in response. Both Chow LPS0 and LPS10 secretions were significantly greater than PN + LPS0. * p<0.05 vs PN+LPS0, † p=0.02.
Figure 4B Representative western blot of sPLA2-IIA in tissue culture media from Chow or PN tissue showed LPS10 increased sPLA-IIA concentrations consistent with the quantified increase in sPLA2 acitivty observed in both groups. Bands are 14 kDa molecular weight.