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. 1984 Jan;81(1):57–60. doi: 10.1073/pnas.81.1.57

Distribution of two selenonucleosides among the selenium-containing tRNAs from Methanococcus vannielii

Wei-Mei Ching 1, Arthur J Wittwer 1, Lin Tsai 1, Thressa C Stadtman 1
PMCID: PMC344609  PMID: 16082779

Abstract

Naturally occurring seleno-tRNAs from Methanococcus vannielii account for 13-20% of the total tRNA population. Two different selenium-modified nucleosides were detected in these seleno-tRNAs. Of the total selenium incorporated, about 60% is present in 5-methylaminomethyl-2-selenouridine, and the other 40% occurs in a second selenonucleoside with spectral characteristics typical of a derivative of 2-selenouridine. The 5-methylaminomethyl-2-selenouridine was found in the seleno-tRNA species present in the early fractions of a linear salt gradient elution profile from a reversed-phase chromatographic system 5(RPC-5) column, whereas the second selenonucleoside occurred in the tRNA species eluted late in the profile.

Keywords: seleno-tRNAs, 5-methylaminomethyl-2-selenouridine, methane-producing bacteria, HPLC of nucleosides

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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