Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Jan;81(1):66–70. doi: 10.1073/pnas.81.1.66

Nucleotide sequence and the encoded amino acids of human apolipoprotein A-I mRNA.

S W Law, H B Brewer Jr
PMCID: PMC344611  PMID: 6198645

Abstract

The cDNA clones encoding the precursor form of human liver apolipoprotein A-I (apoA-I), preproapoA-I, have been isolated from a cDNA library. A 17-base synthetic oligonucleotide based on residues 108-113 of apoA-I and a 26-base primer-extended, dideoxynucleotide-terminated cDNA were used as hybridization probes to select for recombinant plasmids bearing the apoA-I sequence. The complete nucleic acid sequence of human liver preproapoA-I has been determined by analysis of the cloned cDNA. The sequence is composed of 801 nucleotides encoding 267 amino acid residues. PreproapoA-I contains an 18-amino-acid prepeptide and a 6-amino-acid propeptide connected to the amino terminus of the 243-amino acid mature apoA-I. Southern blotting analysis of chromosomal DNA obtained from peripheral blood indicated the apoA-I gene is contained in a 2.1-kilobase-pair Pst I fragment and there is no gross difference in structural organization between the normal apoA-I gene and the Tangier disease apoA-I gene.

Full text

PDF
66

Images in this article

68Image
on p.68
69Image
on p.69

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bell G. I., Karam J. H., Rutter W. J. Polymorphic DNA region adjacent to the 5' end of the human insulin gene. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5759–5763. doi: 10.1073/pnas.78.9.5759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brennan S. O., Carrell R. W. A circulating variant of human proalbumin. Nature. 1978 Aug 31;274(5674):908–909. doi: 10.1038/274908a0. [DOI] [PubMed] [Google Scholar]
  4. Breslow J. L., Ross D., McPherson J., Williams H., Kurnit D., Nussbaum A. L., Karathanasis S. K., Zannis V. I. Isolation and characterization of cDNA clones for human apolipoprotein A-I. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6861–6865. doi: 10.1073/pnas.79.22.6861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brewer H. B., Jr, Fairwell T., Kay L., Meng M., Ronan R., Law S., Light J. A. Human plasma proapoA-I: isolation and amino-terminal sequence. Biochem Biophys Res Commun. 1983 Jun 15;113(2):626–632. doi: 10.1016/0006-291x(83)91772-2. [DOI] [PubMed] [Google Scholar]
  6. Brewer H. B., Jr, Fairwell T., LaRue A., Ronan R., Houser A., Bronzert T. J. The amino acid sequence of human APOA-I, an apolipoprotein isolated from high density lipoproteins. Biochem Biophys Res Commun. 1978 Feb 14;80(3):623–630. doi: 10.1016/0006-291x(78)91614-5. [DOI] [PubMed] [Google Scholar]
  7. Castelli W. P., Doyle J. T., Gordon T., Hames C. G., Hjortland M. C., Hulley S. B., Kagan A., Zukel W. J. HDL cholesterol and other lipids in coronary heart disease. The cooperative lipoprotein phenotyping study. Circulation. 1977 May;55(5):767–772. doi: 10.1161/01.cir.55.5.767. [DOI] [PubMed] [Google Scholar]
  8. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  9. Dugaiczyk A., Law S. W., Dennison O. E. Nucleotide sequence and the encoded amino acids of human serum albumin mRNA. Proc Natl Acad Sci U S A. 1982 Jan;79(1):71–75. doi: 10.1073/pnas.79.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gergen J. P., Stern R. H., Wensink P. C. Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Res. 1979 Dec 20;7(8):2115–2136. doi: 10.1093/nar/7.8.2115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ghiselli G., Schaefer E. J., Light J. A., Brewer H. B., Jr Apolipoprotein A-I isoforms in human lymph: effect of fat absorption. J Lipid Res. 1983 Jun;24(6):731–736. [PubMed] [Google Scholar]
  12. Glomset J. A. The plasma lecithins:cholesterol acyltransferase reaction. J Lipid Res. 1968 Mar;9(2):155–167. [PubMed] [Google Scholar]
  13. Gordon J. I., Sims H. F., Lentz S. R., Edelstein C., Scanu A. M., Strauss A. W. Proteolytic processing of human preproapolipoprotein A-I. A proposed defect in the conversion of pro A-I to A-I in Tangier's disease. J Biol Chem. 1983 Mar 25;258(6):4037–4044. [PubMed] [Google Scholar]
  14. Gordon J. I., Smith D. P., Andy R., Alpers D. H., Schonfeld G., Strauss A. W. The primary translation product of rat intestinal apolipoprotein A-I mRNA is an unusual preproprotein. J Biol Chem. 1982 Jan 25;257(2):971–978. [PubMed] [Google Scholar]
  15. Grosveld F. G., Dahl H. H., de Boer E., Flavell R. A. Isolation of beta-globin-related genes from a human cosmid library. Gene. 1981 Apr;13(3):227–237. doi: 10.1016/0378-1119(81)90028-7. [DOI] [PubMed] [Google Scholar]
  16. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hanahan D., Meselson M. Plasmid screening at high colony density. Gene. 1980 Jun;10(1):63–67. doi: 10.1016/0378-1119(80)90144-4. [DOI] [PubMed] [Google Scholar]
  18. Karathanasis S. K., Norum R. A., Zannis V. I., Breslow J. L. An inherited polymorphism in the human apolipoprotein A-I gene locus related to the development of atherosclerosis. Nature. 1983 Feb 24;301(5902):718–720. doi: 10.1038/301718a0. [DOI] [PubMed] [Google Scholar]
  19. Kay L. L., Ronan R., Schaefer E. J., Brewer H. B., Jr Tangier disease: a structural defect in apolipoprotein A-I (apoA-I Tangier). Proc Natl Acad Sci U S A. 1982 Apr;79(8):2485–2489. doi: 10.1073/pnas.79.8.2485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kunkel L. M., Smith K. D., Boyer S. H., Borgaonkar D. S., Wachtel S. S., Miller O. J., Breg W. R., Jones H. W., Jr, Rary J. M. Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1245–1249. doi: 10.1073/pnas.74.3.1245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Land H., Grez M., Hauser H., Lindenmaier W., Schütz G. 5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency. Nucleic Acids Res. 1981 May 25;9(10):2251–2266. doi: 10.1093/nar/9.10.2251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Law S. W., Dugaiczyk A. Homology between the primary structure of alpha-fetoprotein, deduced from a complete cDNA sequence, and serum albumin. Nature. 1981 May 21;291(5812):201–205. doi: 10.1038/291201a0. [DOI] [PubMed] [Google Scholar]
  23. Law S. W., Gray G., Brewer H. B., Jr cDNA cloning of human apoA-I: amino acid sequence of preproapoA-I. Biochem Biophys Res Commun. 1983 Apr 15;112(1):257–264. doi: 10.1016/0006-291x(83)91824-7. [DOI] [PubMed] [Google Scholar]
  24. Law S., Tamoaki T., Kreuzaler F., Dugaiczyk A. Molecular cloning of DNA complementary to a mouse alpha-fetoprotein mRNA sequence. Gene. 1980 Jun;10(1):53–61. doi: 10.1016/0378-1119(80)90143-2. [DOI] [PubMed] [Google Scholar]
  25. Lin-Su M. H., Lin-Lee Y. C., Bradley W. A., Chan L. Characterization, cell-free synthesis, and processing of apolipoprotein A-I of rat high-density lipoproteins. Biochemistry. 1981 Apr 28;20(9):2470–2475. doi: 10.1021/bi00512a017. [DOI] [PubMed] [Google Scholar]
  26. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Narang S. A., Brousseau R., Hsiung H. M., Michniewicz J. J. Chemical synthesis of deoxyoligonucleotides by the modified triester method. Methods Enzymol. 1980;65(1):610–620. doi: 10.1016/s0076-6879(80)65063-0. [DOI] [PubMed] [Google Scholar]
  28. Nestruck A. C., Suzue G., Marcel Y. L. Studies on the polymorphism of human apolipoprotein A-I. Biochim Biophys Acta. 1980 Jan 18;617(1):110–121. doi: 10.1016/0005-2760(80)90228-3. [DOI] [PubMed] [Google Scholar]
  29. Norum R. A., Lakier J. B., Goldstein S., Angel A., Goldberg R. B., Block W. D., Noffze D. K., Dolphin P. J., Edelglass J., Bogorad D. D. Familial deficiency of apolipoproteins A-I and C-III and precocious coronary-artery disease. N Engl J Med. 1982 Jun 24;306(25):1513–1519. doi: 10.1056/NEJM198206243062503. [DOI] [PubMed] [Google Scholar]
  30. Okayama H., Berg P. High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982 Feb;2(2):161–170. doi: 10.1128/mcb.2.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Osborne J. C., Jr, Brewer H. B., Jr The plasma lipoproteins. Adv Protein Chem. 1977;31:253–337. doi: 10.1016/s0065-3233(08)60220-x. [DOI] [PubMed] [Google Scholar]
  32. Rees A., Shoulders C. C., Stocks J., Galton D. J., Baralle F. E. DNA polymorphism adjacent to human apoprotein A-1 gene: relation to hypertriglyceridaemia. Lancet. 1983 Feb 26;1(8322):444–446. doi: 10.1016/s0140-6736(83)91440-x. [DOI] [PubMed] [Google Scholar]
  33. Rhoads G. G., Gulbrandsen C. L., Kagan A. Serum lipoproteins and coronary heart disease in a population study of Hawaii Japanese men. N Engl J Med. 1976 Feb 5;294(6):293–298. doi: 10.1056/NEJM197602052940601. [DOI] [PubMed] [Google Scholar]
  34. Schaefer E. J., Kay L. L., Zech L. A., Brewer H. B., Jr Tangier disease. High density lipoprotein deficiency due to defective metabolism of an abnormal apolipoprotein A-i (ApoA-ITangier). J Clin Invest. 1982 Nov;70(5):934–945. doi: 10.1172/JCI110705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Shore V., Salen G., Cheng F. W., Forte T., Shefer S., Tint G. S., Lindgren F. T. Abnormal high density lipoproteins in cerebrotendinous xanthomatosis. J Clin Invest. 1981 Nov;68(5):1295–1304. doi: 10.1172/JCI110376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Shoulders C. C., Baralle F. E. Isolation of the human HDL apoprotein A1 gene. Nucleic Acids Res. 1982 Aug 25;10(16):4873–4882. doi: 10.1093/nar/10.16.4873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Smith G. E., Summers M. D. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. Anal Biochem. 1980 Nov 15;109(1):123–129. doi: 10.1016/0003-2697(80)90019-6. [DOI] [PubMed] [Google Scholar]
  38. Sood A. K., Pereira D., Weissman S. M. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer. Proc Natl Acad Sci U S A. 1981 Jan;78(1):616–620. doi: 10.1073/pnas.78.1.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Stoffel W., Blobel G., Walter P. Synthesis in vitro and translocation of apolipoprotein AI across microsomal vesicles. Eur J Biochem. 1981 Dec;120(3):519–522. doi: 10.1111/j.1432-1033.1981.tb05730.x. [DOI] [PubMed] [Google Scholar]
  40. Stoffel W., Krüger E., Deutzmann R. Cell-free translation of human liver apolipoprotein AI and AII mRNA. Processing of primary translation products. Hoppe Seylers Z Physiol Chem. 1983 Mar;364(3):227–237. doi: 10.1515/bchm2.1983.364.1.227. [DOI] [PubMed] [Google Scholar]
  41. Utermann G., Feussner G., Franceschini G., Haas J., Steinmetz A. Genetic variants of group A apolipoproteins. Rapid methods for screening and characterization without ultracentrifugation. J Biol Chem. 1982 Jan 10;257(1):501–507. [PubMed] [Google Scholar]
  42. Weisgraber K. H., Bersot T. P., Mahley R. W., Franceschini G., Sirtori C. R. A-Imilano apoprotein. Isolation and characterization of a cysteine-containing variant of the A-I apoprotein from human high density lipoproteins. J Clin Invest. 1980 Nov;66(5):901–907. doi: 10.1172/JCI109957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Woo S. L., Chandra T., Means A. R., O'Malley B. W. Ovalbumin gene: purification of the coding strand. Biochemistry. 1977 Dec 27;16(26):5670–5676. doi: 10.1021/bi00645a003. [DOI] [PubMed] [Google Scholar]
  44. Zannis V. I., Breslow J. L., Katz A. J. Isoproteins of human apolipoprotein A-I demonstrated in plasma and intestinal organ culture. J Biol Chem. 1980 Sep 25;255(18):8612–8617. [PubMed] [Google Scholar]
  45. Zannis V. I., Kurnit D. M., Breslow J. L. Hepatic apo-A-I and apo-E and intestinal apo-A-I are synthesized in precursor isoprotein forms by organ cultures of human fetal tissues. J Biol Chem. 1982 Jan 10;257(1):536–544. [PubMed] [Google Scholar]
  46. Zannis V. I., Lees A. M., Lees R. S., Breslow J. L. Abnormal apoprotein A-I isoprotein composition in patients with Tangier disease. J Biol Chem. 1982 May 10;257(9):4978–4986. [PubMed] [Google Scholar]
  47. van de Sande J. H., Kleppe K., Khorana H. G. Reversal of bacteriophage T4 induced polynucleotide kinase action. Biochemistry. 1973 Dec 4;12(25):5050–5055. doi: 10.1021/bi00749a004. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES