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. 1984 Jan;81(1):189–192. doi: 10.1073/pnas.81.1.189

Reaction center and light-harvesting I genes from Rhodopseudomonas capsulata

Douglas C Youvan *,†, Marie Alberti *, Helmut Begusch *,‡, Edward J Bylina *,‡, John E Hearst *,‡
PMCID: PMC344636  PMID: 16593406

Abstract

Five structural genes coding for the reaction center (RC) L, M, and H subunits and the two light-harvesting (LH) I polypeptides, B870α and B870β, have been mapped on two restriction fragments from the R-prime plasmid pRPS404. It has been recently shown that enhanced near-infrared fluorescence mutants of Rhodopseudomonas capsulata typically lack RC or LH I polypeptides and that these lesions are marker-rescued by two restriction fragments from the R-prime plasmid: the 7.5-kilobase-pair EcoRI F fragment and the 4.75-kilobase-pair BamHI C-EcoRI fragment. We have now determined the nucleotide sequence of two restriction fragments and have found that the BamHI C-EcoRI B fragment carries the structural genes for the RC L and M subunits and both LH I polypeptides. Forty kilobase pairs away from this locus, the BamHI F fragment (within the EcoRI F fragment) carries the RC H subunit. The structural genes on the BamHI C-EcoRI B fragment are probably transcribed as part of a polycistronic mRNA. All of the structural genes begin with a consensus Shine-Dalgarno sequence and separate AUG start codons, indicating that the structural polypeptides are not cleaved from larger precursor polypeptides.

Keywords: photosynthesis, DNA sequence, enhanced fluorescence mutants, R-prime plasmid, genetic map

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Feick R., Drews G. Isolation and characterization of light harvesting bacteriochlorophyll.protein complexes from Rhodopseudomonas capsulata. Biochim Biophys Acta. 1978 Mar 13;501(3):499–513. doi: 10.1016/0005-2728(78)90117-2. [DOI] [PubMed] [Google Scholar]
  2. Marrs B. Mobilization of the genes for photosynthesis from Rhodopseudomonas capsulata by a promiscuous plasmid. J Bacteriol. 1981 Jun;146(3):1003–1012. doi: 10.1128/jb.146.3.1003-1012.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Pustell J., Kafatos F. C. A high speed, high capacity homology matrix: zooming through SV40 and polyoma. Nucleic Acids Res. 1982 Aug 11;10(15):4765–4782. doi: 10.1093/nar/10.15.4765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Scolnik P. A., Walker M. A., Marrs B. L. Biosynthesis of carotenoids derived from neurosporene in Rhodopseudomonas capsulata. J Biol Chem. 1980 Mar 25;255(6):2427–2432. [PubMed] [Google Scholar]
  6. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sutton M. R., Rosen D., Feher G., Steiner L. A. Amino-terminal sequences of the L, M, and H subunits of reaction centers from the photosynthetic bacterium Rhodopseudomonas sphaeroides R-26. Biochemistry. 1982 Aug 3;21(16):3842–3849. doi: 10.1021/bi00259a019. [DOI] [PubMed] [Google Scholar]
  8. Taylor D. P., Cohen S. N., Clark W. G., Marrs B. L. Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique. J Bacteriol. 1983 May;154(2):580–590. doi: 10.1128/jb.154.2.580-590.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Youvan D. C., Elder J. T., Sandlin D. E., Zsebo K., Alder D. P., Panopoulos N. J., Marrs B. L., Hearst J. E. R-prime site-directed transposon Tn7 mutagenesis of the photosynthetic apparatus in Rhodopseudomonas capsulata. J Mol Biol. 1982 Nov 25;162(1):17–41. doi: 10.1016/0022-2836(82)90160-7. [DOI] [PubMed] [Google Scholar]
  10. Youvan D. C., Hearst J. E., Marrs B. L. Isolation and characterization of enhanced fluorescence mutants of Rhodopseudomonas capsulata. J Bacteriol. 1983 May;154(2):748–755. doi: 10.1128/jb.154.2.748-755.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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