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. 1984 Jan;81(2):405–408. doi: 10.1073/pnas.81.2.405

Chloroplast tRNAGly gene contains a long intron in the D stem: Nucleotide sequences of tobacco chloroplast genes for tRNAGly (UCC) and tRNAArg (UCU)

Hiroshi Deno 1, Masahiro Sugiura 1,*
PMCID: PMC344685  PMID: 16593407

Abstract

The nucleotide sequences of tobacco chloroplast genes for tRNAGly (UCC) and tRNAArg (UCU) have been determined. The tRNAGly gene has a 691-base-pair intron located in the D stem while the tRNAArg gene does not have any intron. The tRNAGly and tRNAArg genes are encoded on the same strand and separated by a 169-base-pair spacer. The tRNAGly gene is transcribed as a 900-base precursor RNA molecule. The tRNAGly and tRNAArg deduced from the DNA sequences show 84% and 55% sequence homologies with Escherichia coli tRNAGly (UCC) and phage T4 tRNAArg (UCU), respectively.

Keywords: molecular cloning, precursor RNA, nuclease S1 mapping, open reading frame

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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