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. 1984 Jan;81(2):434–438. doi: 10.1073/pnas.81.2.434

Cell cycle regulation of human histone H1 mRNA.

M Plumb, F Marashi, L Green, A Zimmerman, S Zimmerman, J Stein, G Stein
PMCID: PMC344691  PMID: 6582499

Abstract

A cloned genomic DNA fragment containing a human histone H1 gene has been used to analyze histone H1 gene expression in two human cell lines (HeLa S3 and WI-38). The cellular abundance of histone H1 mRNA was compared with that of core (H2A, H2B, H3, and H4) histone mRNAs as a function of the cell cycle: core and H1 histone mRNA levels are related both to each other and to the apparent rate of DNA synthesis and are rapidly destabilized after DNA synthesis inhibition. The use of three synchronization protocols, and of transformed and normal diploid cells in culture, suggests that the detected core and H1 histone mRNA levels are regulated by similar mechanisms in continuously dividing human cell lines and nondividing cells stimulated to proliferate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  2. Delegeane A. M., Lee A. S. Coupling of histone and DNA synthesis in the somatic cell cycle. Science. 1982 Jan 1;215(4528):79–81. doi: 10.1126/science.7053561. [DOI] [PubMed] [Google Scholar]
  3. Felsenfeld G. Chromatin. Nature. 1978 Jan 12;271(5641):115–122. doi: 10.1038/271115a0. [DOI] [PubMed] [Google Scholar]
  4. Groppi V. E., Jr, Coffino P. G1 and S phase mammalian cells synthesize histones at equivalent rates. Cell. 1980 Aug;21(1):195–204. doi: 10.1016/0092-8674(80)90127-0. [DOI] [PubMed] [Google Scholar]
  5. Heintz N., Sive H. L., Roeder R. G. Regulation of human histone gene expression: kinetics of accumulation and changes in the rate of synthesis and in the half-lives of individual histone mRNAs during the HeLa cell cycle. Mol Cell Biol. 1983 Apr;3(4):539–550. doi: 10.1128/mcb.3.4.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heintz N., Zernik M., Roeder R. G. The structure of the human histone genes: clustered but not tandemly repeated. Cell. 1981 Jun;24(3):661–668. doi: 10.1016/0092-8674(81)90092-1. [DOI] [PubMed] [Google Scholar]
  7. Hereford L. M., Osley M. A., Ludwig T. R., 2nd, McLaughlin C. S. Cell-cycle regulation of yeast histone mRNA. Cell. 1981 May;24(2):367–375. doi: 10.1016/0092-8674(81)90326-3. [DOI] [PubMed] [Google Scholar]
  8. Hereford L., Bromley S., Osley M. A. Periodic transcription of yeast histone genes. Cell. 1982 Aug;30(1):305–310. doi: 10.1016/0092-8674(82)90036-8. [DOI] [PubMed] [Google Scholar]
  9. Hochhauser S. J., Stein J. L., Stein G. S. Gene expression and cell cycle regulation. Int Rev Cytol. 1981;71:95–243. doi: 10.1016/s0074-7696(08)61183-3. [DOI] [PubMed] [Google Scholar]
  10. Isenberg I. Histones. Annu Rev Biochem. 1979;48:159–191. doi: 10.1146/annurev.bi.48.070179.001111. [DOI] [PubMed] [Google Scholar]
  11. Lawn R. M., Fritsch E. F., Parker R. C., Blake G., Maniatis T. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA. Cell. 1978 Dec;15(4):1157–1174. doi: 10.1016/0092-8674(78)90043-0. [DOI] [PubMed] [Google Scholar]
  12. Lennox R. W., Cohen L. H. The histone H1 complements of dividing and nondividing cells of the mouse. J Biol Chem. 1983 Jan 10;258(1):262–268. [PubMed] [Google Scholar]
  13. Lichtler A. C., Sierra F., Clark S., Wells J. R., Stein J. L., Stein G. S. Multiple H4 histone mRNAs of HeLa cells are encoded in different genes. Nature. 1982 Jul 8;298(5870):195–198. doi: 10.1038/298195a0. [DOI] [PubMed] [Google Scholar]
  14. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Marashi F., Baumbach L., Rickles R., Sierra F., Stein J. L., Stein G. S. Histone proteins in HeLa S3 cells are synthesized in a cell cycle stage specific manner. Science. 1982 Feb 5;215(4533):683–685. doi: 10.1126/science.7058333. [DOI] [PubMed] [Google Scholar]
  16. Moll R., Wintersberger E. Synthesis of yeast histones in the cell cycle. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1863–1867. doi: 10.1073/pnas.73.6.1863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pehrson J. R., Cole R. D. Histone H1 subfractions and H10 turnover at different rates in nondividing cells. Biochemistry. 1982 Feb 2;21(3):456–460. doi: 10.1021/bi00532a006. [DOI] [PubMed] [Google Scholar]
  18. Plumb M., Stein J., Stein G. Coordinate regulation of multiple histone mRNAs during the cell cycle in HeLa cells. Nucleic Acids Res. 1983 Apr 25;11(8):2391–2410. doi: 10.1093/nar/11.8.2391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rickles R., Marashi F., Sierra F., Clark S., Wells J., Stein J., Stein G. Analysis of histone gene expression during the cell cycle in HeLa cells by using cloned human histone genes. Proc Natl Acad Sci U S A. 1982 Feb;79(3):749–753. doi: 10.1073/pnas.79.3.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Robbins E., Borun T. W. The cytoplasmic synthesis of histones in hela cells and its temporal relationship to DNA replication. Proc Natl Acad Sci U S A. 1967 Feb;57(2):409–416. doi: 10.1073/pnas.57.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sierra F., Leza A., Marashi F., Plumb M., Rickles R., Van Dyke T., Clark S., Wells J., Stein G. S., Stein J. L. Human histone genes are interspersed with members of the Alu family and with other transcribed sequences. Biochem Biophys Res Commun. 1982 Jan 29;104(2):785–792. doi: 10.1016/0006-291x(82)90706-9. [DOI] [PubMed] [Google Scholar]
  22. Sierra F., Lichtler A., Marashi F., Rickles R., Van Dyke T., Clark S., Wells J., Stein G., Stein J. Organization of human histone genes. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1795–1799. doi: 10.1073/pnas.79.6.1795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sittman D. B., Graves R. A., Marzluff W. F. Histone mRNA concentrations are regulated at the level of transcription and mRNA degradation. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1849–1853. doi: 10.1073/pnas.80.7.1849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Spalding J., Kajiwara K., Mueller G. C. The metabolism of basic proteins in HeLa cell nuclei. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1535–1542. doi: 10.1073/pnas.56.5.1535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stein G. S., Borun T. W. The synthesis of acidic chromosomal proteins during the cell cycle of HeLa S-3 cells. I. The accelerated accumulation of acidic residual nuclear protein before the initiation of DNA replication. J Cell Biol. 1972 Feb;52(2):292–307. doi: 10.1083/jcb.52.2.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tarnowka M. A., Baglioni C., Basilico C. Synthesis of H1 histones by BHK cells in G1. Cell. 1978 Sep;15(1):163–171. doi: 10.1016/0092-8674(78)90092-2. [DOI] [PubMed] [Google Scholar]
  27. Waithe W. I., Renaud J., Nadeau P., Pallotta D. Histone synthesis by lymphocytes in G0 and G1. Biochemistry. 1983 Apr 12;22(8):1778–1783. doi: 10.1021/bi00277a006. [DOI] [PubMed] [Google Scholar]
  28. Weiner A. M. An abundant cytoplasmic 7S RNA is complementary to the dominant interspersed middle repetitive DNA sequence family in the human genome. Cell. 1980 Nov;22(1 Pt 1):209–218. doi: 10.1016/0092-8674(80)90169-5. [DOI] [PubMed] [Google Scholar]
  29. Wu R. S., Bonner W. M. Separation of basal histone synthesis from S-phase histone synthesis in dividing cells. Cell. 1981 Dec;27(2 Pt 1):321–330. doi: 10.1016/0092-8674(81)90415-3. [DOI] [PubMed] [Google Scholar]
  30. Wu R. S., Tsai S., Bonner W. M. Patterns of histone variant synthesis can distinguish G0 from G1 cells. Cell. 1982 Dec;31(2 Pt 1):367–374. doi: 10.1016/0092-8674(82)90130-1. [DOI] [PubMed] [Google Scholar]

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