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. 1984 Feb;81(4):1070–1074. doi: 10.1073/pnas.81.4.1070

Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.

T Yokota, F Lee, D Rennick, C Hall, N Arai, T Mosmann, G Nabel, H Cantor, K Arai
PMCID: PMC344766  PMID: 6322187

Abstract

A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved using the pcD vector that permits expression of cDNA inserts in mammalian cells. The DNA sequence codes for a polypeptide of 166 amino acid residues including a putative signal peptide. The supernatant fluid obtained after transfection of COS-7 monkey cells with the pcD-MCGF plasmid had mouse MCGF activity. The MCGF amino acid sequence between amino acids 33 and 41, deduced from the nucleotide sequence of its cDNA, is identical to the NH2-terminal sequence of the mouse lymphokine, interleukin 3, suggesting that MCGF is related to interleukin 3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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