Table 1.
Analytical properties of whole Jurkat cells and isolated mitochondria. Reported metal, UV-vis and EPR concentrations refer to packed cells and mitochondria after dividing by packing efficiencies. Values reported for isolated mitochondria were obtained by also dividing measured values by 0.75, to account for presumed metal-free impurities. The absolute uncertainty in percentages obtained by Mössbauer spectroscopy is ± 3%. Replicates n is given in the column to the right of the corresponding parameter. Concentrations of respiration-related mitochondrial proteins are estimated from the collective results of this study.
| Mitochondria | n | Whole Cells | n | |
|---|---|---|---|---|
| Protein (mg/mL) | 52 ± 12 | 4 | 62 ± 11 | 4 |
| [Fe] (μM) | 1120 ± 95 | 5 | 400 ± 70 | 5 |
| [Cu] (μM) | 115 ± 8 | 5 | 28 ± 4 | 5 |
| [Zn] (μM) | 167± 94 | 5 | 408 ± 135 | 5 |
| [Mn] (μM) | 14 ± 3 | 5 | 7.2 ± 0.6 | 5 |
| FeIII oxyhydroxy nanoparticles (%) | 37 | 3 | 18 | 2 |
| Ferritin-like (%) | 15 | 3 | 40 | 2 |
| Central Doublet (%) | 27 | 3 | 27 | 2 |
| Non-Heme HS FeII | 8 | 3 | 11 | 2 |
| HS FeII Hemes | 4 | 3 | 4 | 2 |
| g = 1.94 (μM) | 3.3 ± 0.6 | 4 | 0.3±0.1 | 4 |
| g = 1.90 (μM) | 3.3 ± 0.6 | 4 | 0.3±0.1 | 4 |
| g = 2.00 (μM) | 0.2 ±0.06 | 4 | ~ 0 | 4 |
| g = 4.3 (μM) | 6 ±0.6 | 4 | 1.5 ± 0.5 | 4 |
| g = 6.0 + (6.4, 5.4) | 0.5 ±0.3 | 4 | ~ 0 | 4 |
| Reduced [Heme a] (μM) | 37 ± 5 | 4 | 10 ± 2 | 4 |
| Reduced [Heme b] (μM) | 21 ± 4 | 4 | 5 ± 2 | 4 |
| Reduced [Heme c] (μM) | 75 ± 11 | 4 | 20 ± 3 | 4 |
| Cytochrome c oxidase (μM) | ~ 18 | n/a | ||
| Cytochrome c (μM) | ~ 70 | n/a | ||
| Cytochrome bc1 (μM) | ~ 4 | n/a | ||
| Succinate Dehydrogenase (μM) | ~ 4 | n/a | ||
| Respiratory Complex I (μM) | ~ 1 | n/a |