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. 1999 Jan;29(1):11–25. doi: 10.1023/A:1008075423609

Influence of osmolarity and pH increase to achieve a reduction of monoclonal antibodies aggregates in a production process

Rognoni Franco 1,✉, Gianesello Daniela 1, Maddalena Fabrizio 1, Giuntini Ilaria 1, Herbst Detlev 1
PMCID: PMC3449463  PMID: 19003333

Abstract

Anti PSA monoclonal antibodies for diagnostic use were produced in an in vitro system. After purification using Protein G affinity chromatography a percentage of about 10% of antibody aggregates remained. The use of monoclonal antibodies containing aggregates as a capture antibody in a diagnostic kit reduces the performance of the test making it often unacceptable. The aggregates could be eliminated using gel filtration chromatography but, in that way, the final recovery of the whole production process was only about 50%. Aggregation is favoured when the working pH is near to the isoelectric point of the antibody. We varied the culture medium composition, modifying pH and osmolarity. We tested different values of pH and osmolarity: 7.1, 7.5, 8.0, 8.5 for pH, and 300, 340, 367, 395 mOsm/kg H2O for osmolarity. By modification of the cell culture medium we obtained a significant decrease of monoclonal antibody aggregates in the production cycle. In this way we achieved higher recovery rate and could avoid gel filtration polishing step. The experiments were performed in two stages: first in culture flasks changing one parameter in each experiment, and then in spinner bottle using the best conditions obtained in the first stage. During scale up we used the modifications achieved from the experiment showed in this paper in our production by hollow fibre bioreactor with positive results.

Keywords: aggregates, monoclonal antibodies production, osmolarity, pH

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