Skip to main content
PMC home page
Cytotechnology logoLink to Cytotechnology
. 1999 Nov;31(3):233–242. doi: 10.1023/A:1008030730814

Enhanced CEA production associated with aspirin in a culture of CW-2 cells on some polymeric films

Akon Higuchi 1,✉, Shigeru Uchiyama 1, Makoto Demura 2, Tetsuo Asakura 2, Chong-Su Cho 3, Toshihiro Akaike 4, Hirokazu Takarada 1, Mariko Hara 1
PMCID: PMC3449545  PMID: 19003147

Abstract

Human colorectal adenocarcinoma tumor (CW2) cells were cultivated in RPMI 1640 media containing 0–7.5 mM aspirin and 10% fetal bovine serum for the production of carcinoembryonic antigen (CEA). By adding aspirin to the media, the production of CEA per cell increased by up to one hundred fold compared to cultivation in normal media containing no aspirin, even though the total cell concentration decreased with the increase in aspirin in the media. The production of CEA was also investigated for CW2 cells cultured on silk fibroin, poly(γ-benzyl-L-glutamate) and poly(γ-benzyl-L-glutamate)/poly(ethylene oxide) diblock copolymer films prepared by the Langmuir-Blodgett and casting methods. The highest production of CEA per cell was observed for the CW2 cells on poly(γ-benzyl-L-glutamate) and its diblock copolymer films prepared by the Langmuir-Blodgett method in the medium containing 5 mM aspirin after 168 hr of inoculation. This originates from the fact that the cell density on the films in the medium containing 5 mM aspirin was the lowest under these conditions. It is suggested that CW2 cells produce CEA more effectively when the cell growth is suppressed by addition of toxic chemicals such as aspirin or by culture on unfavorable films for cell growth.

Keywords: cell culture, carcinoembryonic antigen, aspirin, enhanced production, Langmuir-Blodgett film

Full Text

The Full Text of this article is available as a PDF (183.5 KB).

References

  1. Al-Rubeai M, Enery AW. Mechanisms and kinetics of monoclonal antibody synthesis and secretion in synchronous and asynchronous hybridoma cell cultures. J. Biotechnol. 1990;16:67–86. doi: 10.1016/0168-1656(90)90066-K. [DOI] [PubMed] [Google Scholar]
  2. Cho CS, Kim SW, Komoto T. Synthesis and structural study of an ABA block copolymer consisting of poly(γ-benzyl-L-glutamate) as the A block and poly(ethylene oxide) as the B block. Makromol. Chem. 1990;191:981. doi: 10.1002/macp.1990.021910423. [DOI] [Google Scholar]
  3. Cho CS, Kobayashi A, Goto M, Park KH, Akaike T. Difference in adhesion and proliferation of fibroblast between Langmuir-Blodgett films and cast surfaces of poly(γ-benzyl L-glutamate)/poly(ethylene oxide) diblock copolymer. J. Biomed. Mater. Res. 1996;32:425–432. doi: 10.1002/(SICI)1097-4636(199611)32:3<425::AID-JBM16>3.0.CO;2-F. [DOI] [PubMed] [Google Scholar]
  4. David GS, Reisfeld RA, Chino TH. Continuous production of carcinoembryonic antigen in hollow fiber cell culture units: brief communication. J. Natl. Cancer Inst. 1978;60:303–306. doi: 10.1093/jnci/60.2.303. [DOI] [PubMed] [Google Scholar]
  5. Fong W, Zhang Y, Yung Optimization of monoclonal antibody production: combined effects of potassium acetate and perfusion in a stirred tank bioreactor. Cytotechnology. 1997;24:47–54. doi: 10.1023/A:1007914004727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gentry A, Morse PA, Potter VR. Pyridine metabolism in tissue culture cells derived from rat hepatomas. III. Relationship of thymidine to the metabolism of other pyrimidine nucleosides in suspension cultures derived from the Novikoff hepatoma. Cancer Res. 1965;25:517–525. [PubMed] [Google Scholar]
  7. Higuchi A, Takarada H, Uchino J, Tanigaki Y, Hara M. Hollow fiber bioreactor for high density and continuous production of carcinoembryonic antigen. Sen'i Gakkaishi. 1998;54:301–308. [Google Scholar]
  8. Kim YN, Park YS, Kim HK, Jeon BC, Youn SR, Lee HY. Enhancement of the attachment on microcarriers and tPA production by fibroblast cells in a serum-free medium by the addition of the extracts of Centella asiatica. Cytotechnology. 1993;13:221–226. doi: 10.1007/BF00749818. [DOI] [PubMed] [Google Scholar]
  9. Lee GM, Kim SJ, Palsson BO. Enhanced specific productivity of calcium alginate-entrapped hybridoma is cell line-specific. Cytotechnology. 1994;16:1–15. doi: 10.1007/BF00761774. [DOI] [PubMed] [Google Scholar]
  10. Mochizuki K, Sato S, Kato M, Hashizume S. Enhanced production of human monoclonal antibodies by the use of fructose in serum-free hybridoma culture media. Cytotechnology. 1993;13:161–73. doi: 10.1007/BF00749812. [DOI] [PubMed] [Google Scholar]
  11. Murakami F, Sasaki T, Sugahara T. Lysozyme stimulates immunoglobulin production by human-human hybridoma and human peripheral blood lymphocytes. Cytotechnology. 1997;24:177–182. doi: 10.1023/A:1007936629501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Quarles JM, Morris NG, Leibovitz A. Carcinoembryonic antigen production by human colorectal adenocarcinoma cells in matrix-perfusion culture. In vitro. 1980;16:113–118. doi: 10.1007/BF02831502. [DOI] [PubMed] [Google Scholar]
  13. Rutzky LP, Tomita JT, Calenoff MA, Kahan BD. Human colon adenocarcinoma cells. III. In vitro organoid expression and carcinoembryonic antigen kinetics in hollow fiber culture. J. Natl. Cancer Inst. 1979;63:893–902. [PubMed] [Google Scholar]
  14. Somenshein GE, Brawerman G. Differential translation of mouse myeloma messenger RNAs in a wheat germ cell-free system. Biochemistry. 1976;15:5501–5506. doi: 10.1021/bi00670a013. [DOI] [PubMed] [Google Scholar]
  15. Sugahara T, Shirahata S, Yamada K, Murakami H. Purification of immunoglobulin production stimulating factor-IIα derived from Namalwa cells. Cytotechnology. 1991;5:255–263. doi: 10.1007/BF00556295. [DOI] [PubMed] [Google Scholar]
  16. Suzuki E, Ollis DF. Enhanced antibody production at slowed growth rates: Experimental demonstration and a structured model. Biotechnol. Prog. 1990;6:231–236. doi: 10.1021/bp00003a013. [DOI] [PubMed] [Google Scholar]
  17. Suzuki E, Terada S, Ueda H, Fujita T, Komatsu T, Takayama S, Reed JC. Establishing apoptosis resistant cell lines for improving protein productivity of cell culture. Cytotechnology. 1997;23:55–59. doi: 10.1023/A:1007942929800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takahashi K, Tereda S, Ueda H, Makishima F, Suzuki E. Growth rate suppression of cultured mammalian cells enhances protein productivity. Cytotechnology. 1994;15:57–64. doi: 10.1007/BF00762379. [DOI] [PubMed] [Google Scholar]
  19. Toyoda K, Sugahara T, Inoue K, Yamada K, Shirahata S, Murakami H. Purification and characterization of the immunoglobulin production stimulating factor derived from human B lymphoblastoid cell HO-323. Cytotechnology. 1990;3:189–197. doi: 10.1007/BF00143681. [DOI] [PubMed] [Google Scholar]
  20. Wakuri S, Izumi J, Sasaki K, Tanaka N, Ono H. Cytotoxicity study of MEIC chemicals by colony formation and ATP assays. Toxic in Vitro. 1993;7:517–521. doi: 10.1016/0887-2333(93)90057-C. [DOI] [PubMed] [Google Scholar]
  21. Yamaguchi M, Shirai Y, Inouye Y, Shoji M, Kamei M, Hashizume S, Shirahata S. Changes in monoclonal antibody productivity of recombinant BHK cells immobilized in collagen gel particles. Cytotechnology. 1997;23:5–12. doi: 10.1023/A:1007959400666. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Cytotechnology are provided here courtesy of Springer Science+Business Media B.V.

RESOURCES