Skip to main content
Cytotechnology logoLink to Cytotechnology
. 2000 Nov;34(3):197–204. doi: 10.1023/A:1008162703957

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin

Noriko Fukuda 1, Hiroyuki Tanaka 1, Yukihiro Shoyama 2,
PMCID: PMC3449635  PMID: 19003395

Abstract

The ratio of hapten and bovine serum albumin in an antigenconjugate was determined by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Ahybridoma secreting monoclonal antibody against ginsenosideRg1 was produced by fusing sprenocytes immunized with aginsenoside Rg1-bovine serum albumin conjugate withHAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very smallcross-reaction appeared with ginsenoside Re. The full measuringrange of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 ofginsenoside Rg1.

Keywords: ELISA, ginsenoside Rg1, mass spectrometry, monoclonal antibody, qualitative analysis

Full Text

The Full Text of this article is available as a PDF (101.7 KB).

References

  1. Besso H, Kasai R, Saruwatari Y, Fuwa T, Tanaka O. Ginsenoside-Ra1 and ginsenoside-Ra2, new dammaranesaponins of ginseng roots. Chem Pharm Bull. 1982;30:2380–2385. [Google Scholar]
  2. Erlanger BF, Beiser SM. Antibodies specific for ribonucleosides and ribonucleotides and their reaction with DNA. Proc Natl Acad Sci USA. 1964;52:68–74. doi: 10.1073/pnas.52.1.68. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Galfre G, Milstein C. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 1981;73:3–46. doi: 10.1016/0076-6879(81)73054-4. [DOI] [PubMed] [Google Scholar]
  4. Goding JW. Antibody production by hybridomas. J Immunol Meth. 1980;39:285–308. doi: 10.1016/0022-1759(80)90230-6. [DOI] [PubMed] [Google Scholar]
  5. Goto Y, Shima Y, Morimoto S, Shoyama Y, Murakami H, Kusai A, Nojima K. Determination of tetrahydrocannabinolic acid-carrier protein conjugate by matrix-assisted laser desorption/ionization mass spectrometry and antibody formation. Org Mass Spectr. 1994;29:668–671. [Google Scholar]
  6. Hansen L, Boll PM. Polyacetylenes in Araliaceae: Their chemistry, biosynthesis and biological significance. Phytochemistry. 1986;25:285–293. [Google Scholar]
  7. Ishiyama M, Shoyama Y, Murakami H, Shinohara H. Production of monoclonal antibodies and development of an ELISA for solamargine. Cytotechnology. 1996;18:153–158. doi: 10.1007/BF00767762. [DOI] [PubMed] [Google Scholar]
  8. Kitagawa I, Taniyama T, Shibuya H, Noda T, Yoshikawa M. Chemical studies on crude drug processing. V. On the constituents of ginseng radix rubra (2): Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root. Yakugaku Zasshi. 1987;107:495–505. doi: 10.1248/yakushi1947.107.7_495. [DOI] [PubMed] [Google Scholar]
  9. Koizumi H, Sanada S, Ida Y, Shoji J. Studies on the saponins of Ginseng. IV. On the structure and enzymatic hydrolysis of ginsenoside-Ra1. Chem Pharm Bull. 1982;30:2393–2398. [Google Scholar]
  10. Murakami H, Masui H, Sato GH, Sueoka N, Chow TP, Kano-Sueoka T. Growth of hybridoma cells in serum-free medium: ethanolamine is an essential component. Proc Natl Acad Sci USA. 1982;79:1158–1162. doi: 10.1073/pnas.79.4.1158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sakata R, Shoyama Y, Murakami H. Production of monoclonal antibodies and enzyme immunoassay for typical adenylate cyclase activator, forskolin. Cytotechnology. 1994;16:101–108. doi: 10.1007/BF00754612. [DOI] [PubMed] [Google Scholar]
  12. Samukawa K, Yamashita H, Matsuda H, Kubo M. Simultaneous analysis of ginsenosides of various ginseng radix by HPLC. Yakugaku Zasshi. 1995;115:241–249. doi: 10.1248/yakushi1947.115.3_241. [DOI] [PubMed] [Google Scholar]
  13. Sankawa U, Sung CK, Han BH, Akiyama T, Kawashima K. Radioimmunoassay for the determination of ginseng saponin, ginsenoside Rg1. Chem Pharm Bull. 1982;30:1907–1910. [Google Scholar]
  14. Shibata S. Chemistry of components in ginseng. J Traditional Sino-Japanese Medicine. 1982;3:62–69. [Google Scholar]
  15. Shoyama Y, Fukada T, Murakami H. Production of monoclonal antibodies and ELISA for thebaine and codeine. Cytotechnology. 1996;19:55–61. doi: 10.1007/BF00749755. [DOI] [PubMed] [Google Scholar]
  16. Shoyama Y, Sakata R, Isobe R, Murakami H. Direct determination of forskolin-bovine serum albumin conjugate by matrix assisted laser desorption ionization. Org Mass Spectr. 1993;28:987–988. [Google Scholar]
  17. Shoyama Y, Fukada T, Tanaka T, Kusai A, Nojima K. Direct determination of opium alkaloid-bovine serum albumin conjugate by matrix assisted laser desorption ionization mass spectrometry. Biol Pharm Bull. 1993;16:1051–1053. doi: 10.1248/bpb.16.1051. [DOI] [PubMed] [Google Scholar]
  18. Shoyama Y, Matsushita H, Zhu XX, Kishira H. Somatic embryogenesis in ginseng (Panax species) In: Bajaj YPS, editor. Biotechnology in Agriculture and Forestry, Vol. 31. Berlin: Springer-Verlag; 1995. pp. 343–356. [Google Scholar]
  19. Shoyama Y, Zhu XX, Nakai R, Shiraishi S. Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets. Plant Cell Rep. 1997;16:450–453. doi: 10.1007/BF01092764. [DOI] [PubMed] [Google Scholar]
  20. Soldati F, Sticher O. HPLC separation and quantitiative determination of ginsenosides from Panax ginseng, Panax quinquefolium and from ginseng drug preparations. 2nd communication. Planta Medica. 1980;39:348–357. doi: 10.1055/s-2008-1074929. [DOI] [PubMed] [Google Scholar]
  21. Sticher O, Soldati F. HPLC separation and quantitative determination of ginsenosides from Panax giseng, Panax quinquefolium and from ginseng drug prepararions (author's transl) Planta Medica. 1979;36:30–42. doi: 10.1055/s-0028-1097237. [DOI] [PubMed] [Google Scholar]
  22. Tanaka Y, Akagi K. Radiation damage. In: Okuda T, Kimura M, Miyamoto A, Wada H, editors. Metabolism and disease supplementary issue, Vol. 29. Tokyo: Nakayama Shoten; 1992. pp. 423–429. [Google Scholar]
  23. Tanaka H, Goto Y, Shoyama Y. Monoclonal antibody based enzyme immunoassay for marihuana (cannabinoid) compounds. J. Immunoassay. 1996;17:321–342. doi: 10.1080/01971529608005796. [DOI] [PubMed] [Google Scholar]
  24. Tanaka H, Putalun W, Tsuzaki C, Shoyama Y. A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine. FEBS Lett. 1997;404:279–282. doi: 10.1016/s0014-5793(97)00106-3. [DOI] [PubMed] [Google Scholar]
  25. Tanaka H, Fukuda N, Shoyama Y. Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization. Cytotechnology. 1999;29:115–120. doi: 10.1023/A:1008068718392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tani T, Kubo M, Katsuki T, Higashino M, Hayashi T, Arichi S. Histochemistry II. Ginsenosides in Ginseng (Panax ginseng root) J Nat Prod. 1981;44:401–407. [Google Scholar]
  27. Tomoda M, Hirabayashi K, Shimizu N, Gonda R, Ohara N, Takada K. Characterization of two novel polysaccharides having immunological activities from the root of Panax ginseng. Chem Pharm Bull. 1993;16:1087–1090. doi: 10.1248/bpb.16.1087. [DOI] [PubMed] [Google Scholar]
  28. Weiler EW, Zenk MH. Radioimmunoassay for the determination of digoxin and related compounds in Digitalis lanata. Phytochemistry. 1976;15:1537–1545. [Google Scholar]
  29. Xuan LJ, Tanaka H, Xu Y, Shoyama Y. Preparation of monoclonal antibody against crocin and its characterization. Cytotechnology. 1999;29:65–70. doi: 10.1023/A:1007993615489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yamaguchi H, Kasai R, Matsuura H, Tanaka O, Fuwa T. High-performance liquid chromatographic analysis of acidic saponins of ginseng and related plants. Chem Pharm Bull. 1988;36:3468–3473. doi: 10.1248/cpb.36.3468. [DOI] [PubMed] [Google Scholar]
  31. Yanagihara H, Sakata R, Shoyama Y, Murakami H. Rapid analysis of small samples containing forskolin using monoclonal antibodies. Planta Medica. 1996;62:169–172. doi: 10.1055/s-2006-957844. [DOI] [PubMed] [Google Scholar]
  32. Yanagihara H, Sakata R, Minami H, Tanaka H, Shoyama Y, Murakami H. Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application. Anal Chim Acta. 1996;335:63–70. [Google Scholar]
  33. Yoon SR, Nah JJ, Kim SK, Kim SC, Nam KY, Jung DW, Nah SY. Determination of ginsenoside Rf and Rg2 from Panax ginseng using enzyme immunoassasy. Chem Pharm Bull. 1998;46:1144–1147. doi: 10.1248/cpb.46.1144. [DOI] [PubMed] [Google Scholar]

Articles from Cytotechnology are provided here courtesy of Springer Science+Business Media B.V.

RESOURCES