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. 2002 Sep;39(3):163–170. doi: 10.1023/A:1023949716842

Microsupport with two-dimensional geometry (2D-MS) 4. Temperature-induced detachment of anchorage-dependent CHO-K1 cells from cryoresponsive MicroHex® (CryoHex)

N Kenda-Ropson 1, S Lenglois 1, AOA Miller 1
PMCID: PMC3449639  PMID: 19003309

Abstract

Tissue culture flasks were activated by electron beam irradiation and subsequently treated with N-isopropylacrylamide to make them cryoresponsive. Leaving such ‘cryoflasks’ unattended for 10 minutes at room temperature sufficed to almost completely detach the anchorage-dependent cells. MicroHex®, a polystyrene-based tissue culture microsupport with two-dimensional geometry, was handled in the same way to obtain CryoHex, i.e. a cryoresponsive MicroHex from which anchorage-dependent cells could be detached by exposure to low temperature (4–20 °C). Experimental conditions were determined allowing one to detach the cells from small and large microsupport culture volumes. Cells detached from CryoHex by exposure to low temperature displayed a high cell viability and, upon subcultivation on MicroHex®, did not show any alteration of their growth kinetics.

Keywords: Anchorage-dependent cells, Cryoresponsive, Microhex®, N-isopropylacrylamide

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