Abstract
A modified trypan blue dye exclusion assay was developed usingNi2+ or Co2+ salts to determine the viability ofprimary and transformed cells. When the cells were preincubatedwith NiSO4 or CoCl2 followed by trypan blue assay, thecontrast between stained and unstained cells was significantlyincreased as compared to the conventional trypan blue dyeexclusion assay.
Keywords: CoCl2, NiSO4, sensitivity, trypan blue dye exclusion assay
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