Abstract
In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to reevaluate differences between presently available cellular models for lipogenesis/lipolysis and might provide a new cellular physiological system for studies utilizing the proliferative progeny from mature adipocyte dedifferentiation. Alternative possibilities to the dedifferentiation phenomenon are proposed, as this new area of research is novel.
Keywords: Adipofibroblasts, Ceiling culture, Cell culture, Dedifferentiation, Mature adipocytes
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Glossary
- DMEM
Dulbecco’s modified Eagle medium
- DMEM/F12
1:1 ratio; Dulbecco’s modified Eagle medium + Ham’s F12
- FBS
fetal bovine serum
- HBSS
Hank’s balanced salt solution
- HS
horse serum
- PBS
phosphate buffered saline, pH 7.08
- PSG
pigskin gelatin
- SC
satellite cell
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