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. 1984 Mar;81(6):1639–1643. doi: 10.1073/pnas.81.6.1639

Purification in a functional form of the terminal protein of Bacillus subtilis phage phi 29.

I Prieto, J M Lázaro, J A García, J M Hermoso, M Salas
PMCID: PMC344973  PMID: 6424120

Abstract

Phage phi 29 terminal protein, p3, essentially pure, was isolated in a denatured form from viral particles, and anti-p3 antiserum was obtained. A radioimmunoassay to detect and quantitate protein p3 was developed. By using this assay, native protein p3 was highly purified from Escherichia coli cells harboring a gene 3-containing recombinant plasmid. After three purification steps, the protein was more than 96% pure; its amino acid composition was very similar to that deduced from the nucleotide sequence of gene 3. The purified protein was active in the formation of the covalent p3-dAMP initiation complex when supplemented with extracts of B. subtilis infected with a sus mutant of phi 29 in gene 3. No DNA polymerase or ATPase activities were present in the final preparation of protein p3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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