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. 2003 Jan;41(1):45–49. doi: 10.1023/A:1024228932488

The use of foetal ovarian stromal cell culture for cytogenetic diagnosis. Stromal ovarian culture cytogenetic diagnosis

I Roig 1, I Vanrell 1, A Ortega 1, Ll Cabero 2, J Egozcue 3, M Garcia 3
PMCID: PMC3449756  PMID: 19002961

Abstract

Some studies have been carried out to analyze human female first meiotic prophase. Most of them use samples from foetuses collected after legal interruption of pregnancy. In some cases, a control population is needed and foetuses aborted for non-chromosomal reasons are used. The assumption of these samples as being euploids could perhaps represent an error. In this article, we describe an easy methodology to certify the euploidy of foetal ovarian tissue using an one-week somatic culture. Using this protocol, we have obtained a primary culture in 88.2% of the studied cases, material usable for being karyotyped in 93.3% of the cases, and a cytogenetic diagnosis was performed in 100% of these cases. Finding the same karyotype in cultured cells in cases in which we had a prenatal cytogenetic diagnosis has validated the technique, and in applying this protocol we have been able to check our prophase meiotic-study control population.

Keywords: Cell culture, Cytogenetics, Foetal stroma, Human oocyte, Karyotype, Meiotic prophase

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