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. 1999 Sep;31(1-2):185–193. doi: 10.1023/A:1008084508489

Differential expression of mRNAs encoding laminin chain variants during in vitro development of mouse blastocysts

Mojgan Azimi, Tomoaki Niimi, Naoko Yoshida, Yasuo Kitagawa
PMCID: PMC3449764  PMID: 19003140

Abstract

Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. α1, β1 and γ1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. α4 and α5 mRNAs were additionally expressed after attachment of blastocysts to culture dishes. α2 and β2 mRNAs started to be expressed at the stage of allantois formation. Among ten laminin chains, only the mRNA encoding components of laminin-5 (α3, β3 and γ2) was missing in embryos up to the stage of allantois formation.

Keywords: blastocyst, egg cylinder, embryogenesis, implantation, laminin

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