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. 1999 Sep;31(1-2):165–172. doi: 10.1023/A:1008020121693

The neurite-initiating effect of microbial extracellular glycolipids in PC12 cells

Hiroko Isoda, Hiroshi Shinmoto, Masatoshi Matsumura, Tadaatsu Nakahara
PMCID: PMC3449776  PMID: 19003137

Abstract

The effects of several kinds of microbial extracellular glycolipids on neurite initiation in PC12 cells were examined. Addition of mannosylerythritol lipid-A (MEL-A), MEL-B, and sophorose lipid (SL) to PC12 cells caused significant neurite outgrowth. Other glycolipids, such as polyol lipid (PL), rhamnose lipid (RL), succinoyl trehalose lipid-A (STL-A) and STL-B caused no neurite-initiation. MEL-A increased acetylcholine esterase (AChE) activity to an extent similar to nerve growth factor (NGF). However, MEL-A induced one or two long neurites from the cell body, while NGF induced many neurites. In addition, MEL-A-induced differentiation was transient, and after 48 h, percentage of cells with neurites started to decrease in contrast to neurons induced by NGF, which occurred in a time-dependent manner. MEL-A could induce neurite outgrowth after treatment of PC12 cells with an anti-NGF receptor antibody that obstructed NGF action. These results indicate that MEL-A and NGF induce differentiation of PC12 cells through different mechanisms.

Keywords: biosurfactants, differentiation, microbial extracellular glycolipids, PC12

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