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. 2003 Jul;42(2):101–107. doi: 10.1023/B:CYTO.0000009919.62901.27

Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies

Hiroyuki Tanaka 1, Shayu Yan 1, Norio Miura 2, Yukihiro Shoyama 1,✉
PMCID: PMC3449795  PMID: 19002932

Abstract

The ratios of hapten and bovine serum albumin (BSA) in an antigen conjugate were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Hybridomas secreting monoclonal antibodies against 2,4-dichlorophenoxyacetic acid (2,4-D) were produced by fusing 2,4-D-BSA conjugate-immunized splenocytes with a HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A substantial cross-reaction was observed for 2,4-dichlorophenol (2,4-DP) when compared with that observed for 2,4-D. The full measurement range for this assay is 0.2–3 μg ml−1 for 2,4-DP. On the other hand, the range for 2,4-D is between 1 and 20 μg ml−1.

Keywords: Assay system; Biomedical application; 2,4-Dichlorophenol; 2,4-Dichlorophenoxyacetic acid; ELISA; Monoclonal antibody

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