Abstract
A new cell culture supplement, platelet lysate, was evaluated with reference to fetal bovine serum (FBS), an established industrial medium for animal cell culture. Chemical and bacteriological profiles were conducted including the presence of platelet-derived growth factor (PDGF). PDGF was detected in the platelet lysate but it was not present in FBS. The platelet lysate medium demonstrated lack of microorganisms, mycoplasma and endotoxins. The platelet lysate was investigated in culture studies (cell growth, viability and product formation) towards a number of target cells including myelomas, hybridomas, hepatocytes, fibroblasts and epithelial cells. In general the platelet lysate medium supported cell growth and maintained viabilities comparable or superior to fetal bovine serum. Productivity studies of antibodies (hybridomas) and transferrin (hepatocytes) showed similar or enhanced production in platelet-derived medium in comparison with FBS.
Keywords: Animal cell culture, Fetal bovine serum, Growth supplement, Platelet lysate and Roller bottle
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References
- Chen Z.L., Xiao C.Z., Liu H., Wu B.C., Jia X.H. A novel serum-free medium for the cultivation of vero cells on microcarriers. Biotechnol. Tech. 1996;10(6):449–452. [Google Scholar]
- Fredriksson S., Gullberg M., Jarvius J., Olsson C., Pietras K., Gustafsdottir S.M., Ostman A., Landegren U. Protein detection using proximity-dependent DNA ligation assays. Nature Biotechnol. 2002;20(5):473–477. doi: 10.1038/nbt0502-473. [DOI] [PubMed] [Google Scholar]
- Fukimizu H., Grinell F. Spatial organization of extracellular matrix and fibroblast activity: effects of serum, transforming growth factor, and fibronectin. Exp. Cell Res. 1990;190:276–282. doi: 10.1016/0014-4827(90)90197-I. [DOI] [PubMed] [Google Scholar]
- Hodgson J. To treat or not to treat: that is the question for serum. Biotechnology. 1995;13(4):333–334. doi: 10.1038/nbt0495-333. [DOI] [PubMed] [Google Scholar]
- Holmqvist O. and Westermark B. 1989. Blood platelet lysate, method of its preparation and a cell culture medium containing said blood platelet lysate. European Patent Cooperation 413727.
- Lebkowski J.S., Schain L.R., Okarma T.B. Serum-free culture of hematopoietic stem cells: a review. Stem Cells. 1995;13(6):607–612. doi: 10.1002/stem.5530130605. [DOI] [PubMed] [Google Scholar]
- Moreira J.L., Alves P.M., Feliciano A.S., Aunins J.G., Carrondo M.J.T. Serum-free and serum-containing media for growth of suspended BHK aggregates in stirred vessels. Enzyme Microbial Technol. 1995;17(5):437–444. doi: 10.1016/0141-0229(94)00071-X. [DOI] [Google Scholar]
- Ross R., Raines E.W. Platelet-derived growth factor andcellproliferation. In: Sara V.R., editor. GrowthFactors:From Genes to Applications. New York: Raven Press; 1990. pp. 193–200. [Google Scholar]
- Sporn M.B., Roberts A.B. Transforming growth factor-beta: multiple actions and potential applications. J. Am. Med. Assn. 1989;262(7):938–941. doi: 10.1001/jama.262.7.938. [DOI] [PubMed] [Google Scholar]
- Tezel T.H., Priore L.V.D. Serum-free media for culturing and serial-passaging of adult human retinal pigment epithelium. Exp. Eye Res. 1998;66(6):807–815. doi: 10.1006/exer.1998.0492. [DOI] [PubMed] [Google Scholar]