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. 2002 May;39(1):27–35. doi: 10.1023/A:1022496703875

Effects of cetyltriethylammonium bromide on the replication of Bombyx mori nucleopolyhedrovirus

Yajing Zhou 1,2, Zhifang Zhang 1,✉, Jialu He 1, Yuanxing Zhang
PMCID: PMC3449800  PMID: 19003301

Abstract

An experimental study was undertaken to quantify the effects of cetyltriethylammonium bromide (CTAB) on the replication of Bombyx mori nucleopolyhedrovirus (BmNPV) and the transcriptionalactivity of BmNPV ie-1 promoter. The results demonstrated that the budded virus (BV) titer rose about 3.7-fold by adding CTAB to the culture media up to 0.1 μ g ml-1 in infected Bm-N cells with a wild-type BmNPV. The transient expression level of luciferase driven by BmNPV ie-1 promoter was enhanced by more than 3-fold in the presence of 0.1 μ g ml-1 of CTAB in uninfected insect cells via a transient expression system. Contrary to the rise in BV titer, the polyhedra inside the nucleus of infected cells dropped linearly from 4.0 × 106 ml-1 down to 2.1 × 106 ml-1 with in a range of CTAB concentrations from 0 to 0.25 μ g ml-1. The same trend in expression level of β -galactosidase or phytase was given when the Bm-N cells or fifth-instar silkworm larvae infected with a recombinant BmNPV containing the β -galactosidase or phytase reporter gene driven by the polyhedrin promoter. We deduced that CTAB appeared to affect the virus bi-phasic life cycle stages and production pathways, resulting in an enhancement in BV production and a suppression of occluded virus (OV) production and expression of foreign genes controlled by the polyhedrin promoter.

Keywords: BmNPV, CTAB, infection, insect cells, silkworm larvae, transfection

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