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. 2001 Sep;37(1):13–22. doi: 10.1023/A:1016195029359

Explants of porcine coronary artery in culture: A paradigm for studying the influence of heparin on vascular wall cell proliferation

M Dufresne 1,✉, R Warocquier-Clérout 2
PMCID: PMC3449968  PMID: 19002910

Abstract

Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. Percentages of anti-smooth muscle α-actin labelled cells were assessed at early and late phase by flow cytofluorometric analysis to control the effect of heparin. At 100 μg ml-1, no effect on α-actin labelled cell growth has been detected. This result contrasted with the inhibition of monolayer cell cultures. At 500 μg ml-1, the proliferation of smooth muscle cells was reduced. This explant system should be useful for testing drugs susceptible to interfere with restenosis.

Keywords: coronary artery explant culture, endothelial cells, heparin, proliferation, smooth muscle cells

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