Abstract
We reported the optimum amount of PHB accumulated by Alcaligenes faecalis during its 24 h growth under nitrogen deficient conditions. After 24 h incubation decrease in the amount of PHB was recorded. Hypochlorite digestion of biomass of organism followed by extraction with a solvent system consisting of 1:1 mixture of ethanol and acetone resulted in efficient recovery of PHB vis-à-vis earlier methods. This solvent system gave a high recovery yield, i.e. 5.6 gL−1 vis-à-vis earlier reported yield, 1.34 gL−1 (by same method), 0.63 gL−1 (by chloroform extraction method) and 1.1 gL−1 (by dispersion method).
Keywords: Alcaligenes faecalis, PHB, Hypochlorite, NPCM
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