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. 2008 Jan 11;47(4):358–363. doi: 10.1007/s12088-007-0064-0

Periplasmically located α-santonin binding factor in Sphingomonas paucimobilis strain S ATCC 43388

M Shailaja Raj 1,✉, I Furtado 2, S Mavinkurve 2
PMCID: PMC3450028  PMID: 23100690

Abstract

A marked reduction in uptake of α-santonin, accompanied by loss of ability of cells to transform the substrate, is observed on shocking Sphingomonas paucimobilis strain S ATCC 43388 cells by freeze — thaw method. The shock fluid shows a 26% quench in fluorescence at 350nm on incubation with the substrate. Addition of shock fluid to the freeze thawed cells restores both uptake as well as transformation of α-santonin to near normal.

Keywords: Periplasmic proteins, Shock fluid, Fluorescence, Sphingomonas

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