Abstract
Forty-six Rhizobium isolates from legume root and stem nodules were examined for their phosphate-solubilizing ability on Pikovskaya’s agar medium. Rhizobium isolates from root nodules of Cassia absus, Vigna trilobata and three strains from Sesbania sesban showed zone of tricalcium phosphate (TCP) solubilization. The isolate from C. absus showed maximum solubilization (620 μg/ml) after 12 d of incubation, while the Rhizobium sp. strain 26 (from S. sesban) showed the least amount (150 μg/ml) of phosphate solubilization. Among the carbon sources tested for their ability to solubilize TCP, maximum solubilization (620 μg/ml) was observed in glucose by Rhizobium isolate from C. absus. Phosphate solubilization increased with increase in glucose concentration steeply up to 2% and slowly above this concentration in four isolates. Among the nitrogen sources tested, maximum solubilization (620 μg/ml) was observed in ammonium sulphate by Rhizobium isolate from C. absus.
Keywords: Phosphate solubilization, Rhizobium, Tricalcium phosphate
Full Text
The Full Text of this article is available as a PDF (582.1 KB).
References
- 1.Haque N.A., Dave S.R. Ecology of phosphate solubilizers in semi-arid agricultural soils. Indian J Microbiol. 2005;45:27–32. [Google Scholar]
- 2.Alikhani H.A., Saleh-Rastin N., Antoun H.Phosphate solubilization activity of rhizobia native to Iranian soils Plant Soil 200628735–41.10.1007/s11104-006-9059-6 [Google Scholar]
- 3.Deshwal V.K., Dubey R.C., Maheshwari D.K. Isolation of plant-growth promoting strains of Bradyrhizobium (Arachis) sp. with biocontrol potential against Macrophomina phaseolina causing charcoal rot of peanut. Curr Sci. 2003;84:443–448. [Google Scholar]
- 4.Halder A.K., Mishra A.K., Bhattacharya P., Chakrabarty P.K. Solubilization of inorganic phosphate by Rhizobium. Indian J Microbiol. 1990;30:311–314. [Google Scholar]
- 5.Haldar A.K., Mishra A.K., Bhattacharya P., Chakrabarty P.K. Solubilization of inorganic phosphates by Bradyrhizobium. Indian J Exp Biol. 1991;29:28–31. [Google Scholar]
- 6.Halder A.K., Chakrabarty P.K.Solubilization of inorganic phosphate by Rhizobium Folia Microbiol 199338325–330.10.1007/BF02898602 [Google Scholar]
- 7.Rivas R., Peix A., Mateos P.F., Trujillo M.E., Martinez-Molina E., Velazquez E.Biodiversity of populations of phosphate solubilizing rhizobia that nodulates chickpea in different Spanish soils Plant Soil 200628723–33.10.1007/s11104-006-9062-y [Google Scholar]
- 8.Daimon H., Nobuta K., Ohe M., Harada J., Nakayama Y.Tricalcium phosphate solubilization by root nodule bacteria of Sesbania cannabina and Crotalaria juncea Plant Prod Sci 20069388–389.10.1626/pps.9.388 [Google Scholar]
- 9.Peix A., Rivas-Boyero A.A., Mateos P.F., Rodriguez-Barrueco C., Martinez-Molina E., Velazquez E.Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions Soil Biol Biochem 200133103–110.10.1016/S0038-0717(00)00120-6 [Google Scholar]
- 10.Vincent J.M. I.B.P. Handbook No. 15. Oxford, England: Blackwell Scientific Publications; 1970. A manual for the practical study of root nodule bacteria; pp. 73–97. [Google Scholar]
- 11.Somasegaran P., Hoben H.J. Handbook for rhizobia — methods in legume-rhizobium Technology. New York: Springer-Verlag; 1994. [Google Scholar]
- 12.Kuykendall L.D., Young J.M., Martinez-Romero E., Kerr A., Sawada H. Family I. Rhizobiaceae. Genus I. Rhizobium. In: Brenner D.J., Krieg N.R., Staley J.T., editors. Bergey’s manual of systematic bacteriology. 2nd edn. New York, USA: Springer Science; 2005. [Google Scholar]
- 13.Pikovskaya R.I. Mobilization of phosphorus in soil in connection with vital capacity of source microbial species. Microbiologiya. 1948;17:362–370. [Google Scholar]
- 14.Srivastava S., Yadav K.S., Kundu B.S. Prospects of using phosphate solubilizing Pseudomonas as biofungicide. Indian J Microbiol. 2004;44:91–94. [Google Scholar]
- 15.Jackson M.L. Soil Chemical Analysis. New Delhi, India: Prentice Hall of India; 1973. pp. 134–182. [Google Scholar]
- 16.Dave A., Patel H.H. Inorganic phosphate solubilizing Psueudomanas. Indian J Microbiol. 1999;39:161–164. [Google Scholar]
- 17.Rodriguez H., Gonzalez T., Goire I., Bashan Y. Gluconic acid production and phosphate solubilization by the plant growth-promoting bacterium Azospirillum spp. Naturwissenschaften. 2004;91:552–555. doi: 10.1007/s00114-004-0566-0. [DOI] [PubMed] [Google Scholar]