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. 2010 Oct 30;50(3):280–291. doi: 10.1007/s12088-010-0062-5

Species confirmation of fungal isolates by molecular analysis

Rupesh Thakur 1,✉, Sardul S Sandhu 2
PMCID: PMC3450067  PMID: 23100842

Abstract

Traditional taxonomy of hyphomycetes has been based on conidial morphology and development. In order to confirm species level for the detection and identification of the entomopathogenic fungus, we analysed the species-specific fingerprints to investigate molecular characteristics within isolates of six species and to resolve morphologically atypical isolates. The extent of fingerprint profile observed by RAPD was sufficient to confirm the species level of all the isolates. The genetic similarity among morphologically identified isolates of each species was considerably higher, allowing us to conclude that all the isolates are of same species. These results establish a molecular framework for further taxonomic, phylogenetic and comparative biological investigations.

Keywords: Beauveria bassiana, Entomopathogenic fungi, Isaria felina, Metarhizium anisopliae, Nomuraea rileyi

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Contributor Information

Rupesh Thakur, Email: rupeshthakur47@gmail.com.

Sardul S. Sandhu, Email: scientist@acadrnd.peoplesgroup.in

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