Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Mar;81(6):1809–1812. doi: 10.1073/pnas.81.6.1809

Shared idiotopes among monoclonal antibodies specific for A/PR/8/34 (H1N1) and X-31(H3N2) influenza viruses.

T Moran, Y C Liu, J L Schulman, C A Bona
PMCID: PMC345010  PMID: 6584913

Abstract

A monoclonal antibody specific for the hemagglutinin (HA) of influenza A X-31 (H3N2) virus (X-31) was obtained during a fusion of spleen cells from a BALB/c mouse immunized with influenza A/PR/8/34 (H1N1) virus (PR8). This monoclonal antibody (Py206) shares crossreactive idiotopes expressed on several monoclonal antibodies specific for PR8 HA and X-31 HA as well as an individual idiotope shared with one monoclonal antibody specific for X-31 HA. The presence of shared idiotypy among antibodies of such diverse binding specificities may result from regulation by idiotype-specific T cells. In addition, the results suggest that, in contrast to the diversity of paratypes expressed, relatively few germ-line variable region genes may be used in the antibody response to influenza A virus antigens.

Full text

PDF
1809

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binz H., Wigzell H. Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. I. Demonstration of similar or identical idiotypes on IgG molecules and T-cell receptors with specificity for the same alloantigens. J Exp Med. 1975 Jul 1;142(1):197–211. doi: 10.1084/jem.142.1.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bona C., Mond J. J., Stein K. E., House S., Lieberman R., Paul W. E. Immune response to levan. III. The capacity to produce anti-inulin antibodies and cross-reactive idiotypes appears late in ontogeny. J Immunol. 1979 Oct;123(4):1484–1490. [PubMed] [Google Scholar]
  3. Bona C., Paul W. E. Cellular basis of regulation of expression of idiotype. I. T-suppressor cells specific for MOPC 460 idiotype regulate the expression of cells secreting anti-TNP antibodies bearing 460 idiotype. J Exp Med. 1979 Mar 1;149(3):592–600. doi: 10.1084/jem.149.3.592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cancro M. P., Wylie D. E., Gerhard W., Klinman N. R. Patterned acquisition of the antibody repertoire: diversity of the hemagglutinin-specific B-cell repertoire in neonatal BALB/c mice. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6577–6581. doi: 10.1073/pnas.76.12.6577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cazenave P. A. L'idiotypie comparée des anticorpus qui, dans le sérum d'un lapin immunisé contre la sérumalbumine humaine, sont dirigés contre des régions différentes de cet antigène. FEBS Lett. 1973 May 1;31(3):348–354. doi: 10.1016/0014-5793(73)80139-5. [DOI] [PubMed] [Google Scholar]
  6. Cerny J., Caulfield M. J. Stimulation of specific antibody-forming cells in antigen-primed nude mice by the adoptive transfer of syngeneic anti-idiotypic T cells. J Immunol. 1981 Jun;126(6):2262–2266. [PubMed] [Google Scholar]
  7. Eichmann K., Coutinho A., Melchers F. Absolute frequencies of lipopolysaccharide-reactive B cells producing A5A idiotype in unprimed, streptococcal A carbohydrate-primed, anti-A5A idiotype-sensitized and anti-A5A idiotype-suppressed A/J mice. J Exp Med. 1977 Nov 1;146(5):1436–1449. doi: 10.1084/jem.146.5.1436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eichmann K., Falk I., Rajewsky K. Recognition of idiotypes in lymphocyte interactions. II. Antigen-independent cooperation between T and B lymphocytes that possess similar and complementary idiotypes. Eur J Immunol. 1978 Dec;8(12):853–857. doi: 10.1002/eji.1830081206. [DOI] [PubMed] [Google Scholar]
  9. Eichmann K., Rajewsky K. Induction of T and B cell immunity by anti-idiotypic antibody. Eur J Immunol. 1975 Oct;5(10):661–666. doi: 10.1002/eji.1830051002. [DOI] [PubMed] [Google Scholar]
  10. Gerhard W., Croce C. M., Lopes D., Koprowski H. Repertoire of antiviral antibodies expressed by somatic cell hybrids. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1510–1514. doi: 10.1073/pnas.75.3.1510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hashimoto G., Wright P. F., Karzon D. T. Antibody-dependent cell-mediated cytotoxicity against influenza virus-infected cells. J Infect Dis. 1983 Nov;148(5):785–794. doi: 10.1093/infdis/148.5.785. [DOI] [PubMed] [Google Scholar]
  12. Hiernaux J., Bona C. A. Shared idiotypes among monoclonal antibodies specific for different immunodominant sugars of lipopolysaccharide of different Gram-negative bacteria. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1616–1620. doi: 10.1073/pnas.79.5.1616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ju S. T., Benacerraf B., Dorf M. E. Genetic control of a shared idiotype among antibodies directed to distinct specificities. J Exp Med. 1980 Jul 1;152(1):170–182. doi: 10.1084/jem.152.1.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Julius M. H., Augustin A. A., Cosenza H. Recognition of a naturally occurring idiotype by autologous T cells. Nature. 1977 Jan 20;265(5591):251–253. doi: 10.1038/265251a0. [DOI] [PubMed] [Google Scholar]
  15. Kunkel H. G., Mannik M., Williams R. C. Individual Antigenic Specificity of Isolated Antibodies. Science. 1963 Jun 14;140(3572):1218–1219. doi: 10.1126/science.140.3572.1218. [DOI] [PubMed] [Google Scholar]
  16. Liu Y. N., Bona C. A., Schulman J. L. Idiotypy of clonal responses to influenza virus hemagglutinin. J Exp Med. 1981 Nov 1;154(5):1525–1538. doi: 10.1084/jem.154.5.1525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lubeck M. D., Schulman J. L., Palese P. Antigenic variants of influenza viruses: marked differences in the frequencies of variants selected with different monoclonal antibodies. Virology. 1980 Apr 30;102(2):458–462. doi: 10.1016/0042-6822(80)90114-2. [DOI] [PubMed] [Google Scholar]
  18. Margolies M. N., Wysocki L. J., Sato V. L. Immunoglobulin idiotype and anti-anti-idiotype utilize the same variable region genes irrespective of antigen specificity. J Immunol. 1983 Feb;130(2):515–517. [PubMed] [Google Scholar]
  19. Noble G. R., Kaye H. S., Kendal A. P., Dowdle W. R. Age-related heterologous antibody responses to influenza virus vaccination. J Infect Dis. 1977 Dec;136 (Suppl):S686–S692. doi: 10.1093/infdis/136.supplement_3.s686. [DOI] [PubMed] [Google Scholar]
  20. Oudin J., Cazenave P. A. Similar idiotypic specificities in immunoglobulin fractions with different antibody functions or even without detectable antibody function. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2616–2620. doi: 10.1073/pnas.68.10.2616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Palese P., Schulman J. Isolation and characterization of influenza virus recombinants with high and low neuraminidase activity. Use of 2-(3'-methoxyphenyl)-n-acetylneuraminic acid to identify cloned populations. Virology. 1974 Jan;57(1):227–237. doi: 10.1016/0042-6822(74)90123-8. [DOI] [PubMed] [Google Scholar]
  22. Palese P., Young J. F. Variation of influenza A, B, and C viruses. Science. 1982 Mar 19;215(4539):1468–1474. doi: 10.1126/science.7038875. [DOI] [PubMed] [Google Scholar]
  23. Staudt L. M., Gerhard W. Generation of antibody diversity in the immune response of BALB/c mice to influenza virus hemagglutinin. I. Significant variation in repertoire expression between individual mice. J Exp Med. 1983 Feb 1;157(2):687–704. doi: 10.1084/jem.157.2.687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Webster R. G. Original antigenic sin in ferrets: the response to sequential infections with influenza viruses. J Immunol. 1966 Aug;97(2):177–183. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES