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Indian Journal of Microbiology logoLink to Indian Journal of Microbiology
. 2007 Jul 8;47(2):144–152. doi: 10.1007/s12088-007-0028-4

Statistical optimization of alkaline xylanase production from Streptomyces violaceoruber under submerged fermentation using response surface methodology

S Khurana 1, M Kapoor 1, S Gupta 1, R C Kuhad 1,✉
PMCID: PMC3450106  PMID: 23100657

Abstract

Response surface methodology employing central composite design (CCD) was used to optimize fermentation medium for the production of cellulase-free, alkaline xylanase from Streptomyces violaceoruber under submerged fermentation. The design was employed by selecting wheat bran, peptone, beef extract, incubation time and agitation as model factors. A second-order quadratic model and response surface method showed that the optimum conditions for xylanase production (wheat bran 3.5 % (w/v), peptone 0.8 % (w/v), beef extract 0.8 % (w/v), incubation time 36 h and agitation 250 rpm) results in 3.0-fold improvement in alkaline xylanase production (1500.0 IUml−1) as compared to initial level (500.0 IUml−1) after 36 h of fermentation, whereas its value predicted by the quadratic model was 1347 IUml−1. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.9718, ensuring a satisfactory adjustment of the quadratic model with the experimental data.

The economical and cellulase-free nature of xylanase would enhance its applicability in pulp and paper industry.

Keywords: Alkaline, CCD, RSM, Streptomyces violaceoruber, Xylanase

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