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. 2007 Jul 8;47(2):139–143. doi: 10.1007/s12088-007-0027-5

Dehalogenation of 4 — Chlorobenzoic Acid by Pseudomonas isolates

G Banta 1,✉, R S Kahlon 1
PMCID: PMC3450107  PMID: 23100656

Abstract

Twenty three bacterial isolates either pure or consortium were initially screened on the basis of their ability to degrade as well as dechlorinate 4 — chlorobenzoic acid (4-CBA). Based on comparative growth response, three pure isolates Pseudomonas putida GVS-4, Pseudomonas aeruginosa GVS-18 and Pseudomonas aeruginosa GWS-19 and a consortium SW-2 was finally selected for further studies. The enzyme studies performed with cell free extracts revealed that dehalogenase activity was substrate specific with maximum activity at 300 μgml−1 substrate concentration. Catechol 1,2 dioxygenase activity was found to be present in cell free extracts suggesting that 4 — chlorobenzoic acid (4-CBA) is catabolized by ortho-ring cleavage pathway. The dehalogenase enzyme profile showed single enzyme band in case of GVS-4 (Rm 0.76), GVS-18 (Rm 0.84), GWS −19 (Rm 0.85) and two bands in SW-2 (Rm 0.71 & 0.10).

Keywords: 4-chlorobenzoic acid, Dehalogenase, Degradation, Pseudomonas putida, Pseudomonas aeruginosa

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References

  • 1.Kalra R.L., Chawla R.P., Sharma M.L., Battu R.S., Gupta S.C. Residues of DDT and HCH in butter and ghee in India, 1979–81. Environ Pollu. 1983;6:195–206. doi: 10.1016/0143-148X(83)90034-4. [DOI] [Google Scholar]
  • 2.Kalra R.L., Chawla R.P. Studies on pesticide residues and monitoring of pesticidal pollution. Ludhiana: Department of Entomology, Punjab Agricultural University; 1983. [Google Scholar]
  • 3.Deo P.G., Karanth N.G., Karanth N.G.K. Microbial degradation of pesticides. Ind J Microbiol. 1994;34:241–263. [Google Scholar]
  • 4.Raizada N (1996) Organochlorine pesticide residues in environmental components, food materials and human tissues. In: International Seminar “Problem of monitoring of pesticide residues in exportable commodities viz. rice, tea, fish and minor crops,” Colombo, Sri Lanka
  • 5.Timmis K.N., Steffan R.J., Unterman R. Designing microorganisms for the treatment of toxic wastes. Ann Rev Microbiol. 1994;48:525–527. doi: 10.1146/annurev.mi.48.100194.002521. [DOI] [PubMed] [Google Scholar]
  • 6.Garbisu C., Alkorta I. Bioremediation. Principles and Future. J Clean Technol Environ Toxicol and Occup Med. 1997;6:1–6. [Google Scholar]
  • 7.Spain R.J. Biodegradation of nitroaromatic compounds. Annu Rev Microbiol. 1995;49:523–555. doi: 10.1146/annurev.mi.49.100195.002515. [DOI] [PubMed] [Google Scholar]
  • 8.Liu S., Suflita J.M. Ecology and evolution of microbial populations for bioremediation. Trends in Biotech. 1998;11:344–352. doi: 10.1016/0167-7799(93)90157-5. [DOI] [PubMed] [Google Scholar]
  • 9.Garbisu C., Alkorta I. Utilization of genetically engineered microorganisms (GEMs) for bioremediation. J Chem Technol Biotechnol. 1999;74:599–606. doi: 10.1002/(SICI)1097-4660(199907)74:7<599::AID-JCTB82>3.0.CO;2-G. [DOI] [Google Scholar]
  • 10.Fetzner S., Lingens F. Bacterial Dehalogenases: Biochemistry, Genetics and Biotechnological Applications. Microbiol Rev. 1994;58:641–685. doi: 10.1128/mr.58.4.641-685.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Singh H., Kahlon R.S. Biochemical and Genetic studies on degradation of chlorobenzoates by Pseudomonas. Acta Microbiologica Polonica. 1989;38:259–269. [PubMed] [Google Scholar]
  • 12.Mohn W.W., Tiedje J.M. Microbial reductive dehalogenation. Microbiol Rev. 1992;56:482–507. doi: 10.1128/mr.56.3.482-507.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Jensen H.L. Decomposition of chloroacetates and chloropropionates by bacteria. Acta Agriculture Scandinavica. 1960;10:83–103. doi: 10.1080/00015126009435651. [DOI] [Google Scholar]
  • 14.Slater J.H., Lovatt D., Weightman A.J., Senior E., Bull A.T. The growth of Pseudomonas putida on chlorinated aliphatic acids and its dehalogenase activity. J Gen Microbiol. 1979;114:125–136. [Google Scholar]
  • 15.Dorn E., Hellwig M., Reineke W., Kanckmuss H.J. Isolation and characterization of a 3-chlorobenzoate degrading. Pseudomonas Arch Microbiol. 1974;99:61–70. doi: 10.1007/BF00696222. [DOI] [PubMed] [Google Scholar]
  • 16.Bergmann J.G., Sainik J. Determination of trace amounts of chlorine in naptha. Analyt Chem. 1957;29:241–243. doi: 10.1021/ac60122a018. [DOI] [Google Scholar]
  • 17.Dorn E., Knackmuss H.J. Chemical structure and biodegradability of halogenated aromatic compounds. Two catechol 1, 2 dioxygenases from a 3-chlorobenzoate grown pseudomonad. Biochem J. 1978;174:73–84. doi: 10.1042/bj1740073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Nozaki M. Metapyrocatechase (Pseudomonas) Methods Enzymo. 1970;17a:522–525. doi: 10.1016/0076-6879(71)17235-7. [DOI] [Google Scholar]
  • 19.Lowry O.H., Rosebrough N.J., Farr A.C., Randall R.J. Protein measurement with Folin-phenol reagent. J Biol Chem. 1951;193:265–273. [PubMed] [Google Scholar]
  • 20.Davis B.J. Disc-gel electrophoresis II: Method and Applications to Human Serum Proteins. Ann NY Acad Sci. 1964;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  • 21.Tros M.E., Scheaa G., Zhender A.J.B. Transformation of low concentration of 3-chlorobenzoate by Pseudomonas sp. strain B13: kinetics and residual concentrations. Appl Environ Microbiol. 1996;62:437–442. doi: 10.1128/aem.62.2.437-442.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Sandhu N. Biochemical and genetic studies on dechlorinases of Pseudomonas sp. Ludhiana, India: PAU; 2002. [Google Scholar]
  • 23.Kocher G.S., Kahlon R.S. Dehalogenation of 2-chlorobenzoic acid by Pseudomonas pickettii. Ind J Microbiol. 1999;39:245–247. [Google Scholar]
  • 24.Hardman D.J., Slater J.H. Dehalogenases in soil bacteria. J Gen Microbiol. 1981;123:117–128. [Google Scholar]
  • 25.Koch A.L. Enzyme evolution. The Importance of Untranslatable Intermediates. Genetics. 1972;72:297–316. doi: 10.1093/genetics/72.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Zaitsev G.M., Karasevich Y. N. Utilization of 2-chlorobenzoic acid by Pseudomonas cepacia. Mikrobiologiya. 1984;53:75–80. [Google Scholar]
  • 27.Fetzner S., Muller R., Lingens F. A novel metabolite in the microbial degradation of 2-chlorobenzoate. Biochem Biophys Res Commun. 1989;161:700–705. doi: 10.1016/0006-291X(89)92656-9. [DOI] [PubMed] [Google Scholar]
  • 28.Sylvestre M., Mailhot K., Ahmad D. Isolation and Preliminary characterization of a 2-chlorobenzoate degrading Pseudomonas. Can J Microbiol. 1989;35:439–443. doi: 10.1139/m89-067. [DOI] [PubMed] [Google Scholar]
  • 29.Kahlon R.S., Singh G. Pseudomonas: Genetics and emerging biotechnology. In: Kahlon R. S., editor. Perspectives in Microbiology. Ludhiana: NATIC Press; 1994. pp. 21–29. [Google Scholar]

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