Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Mar;81(6):1818–1821. doi: 10.1073/pnas.81.6.1818

Stimulation of human granulocyte function by monoclonal antibody WEM-G1.

A F López, M A Vadas
PMCID: PMC345012  PMID: 6584915

Abstract

From a panel of monoclonal antibodies (MAb) produced against purified human neutrophils, MAb WEM-G1 was selected for its ability to stimulate granulocyte function as assessed by the capacity to kill antibody-coated tumor target cells. MAb WEM-G1 bound to neutrophils and eosinophils and not to monocytes, lymphocytes, or erythrocytes, and thus identified a granulocyte differentiation antigen. It enhanced killing by both neutrophils and eosinophils in a dose-dependent fashion, with a range of effector-to-target ratios and dilutions of anti-target cell antibody. The effect of MAb WEM-G1 was additive with that of colony-stimulating factor (CSF-alpha). Similarly, the ability of neutrophils to exhibit enhanced cytotoxicity in the presence of WEM-G1 was increased by preincubation of neutrophils with CSF-alpha. Immunoprecipitation analysis showed that WEM-G1 identified a cell-surface protein on human neutrophils of approximate molecular weight 110,000. It is suggested that this structure is involved in regulation of the function of human granulocytes.

Full text

PDF
1818

Images in this article

1821Image
on p.1821

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crowley C. A., Curnutte J. T., Rosin R. E., André-Schwartz J., Gallin J. I., Klempner M., Snyderman R., Southwick F. S., Stossel T. P., Babior B. M. An inherited abnormality of neutrophil adhesion. Its genetic transmission and its association with a missing protein. N Engl J Med. 1980 May 22;302(21):1163–1168. doi: 10.1056/NEJM198005223022102. [DOI] [PubMed] [Google Scholar]
  2. Dessein A. J., Vadas M. A., Nicola N. A., Metcalf D., David J. R. Enhancement of human blood eosinophil cytotoxicity by semi-purified eosinophil colony-stimulating factor(s). J Exp Med. 1982 Jul 1;156(1):90–103. doi: 10.1084/jem.156.1.90. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  4. Goding J. W. Structural studies of murine lymphocyte surface IgD. J Immunol. 1980 May;124(5):2082–2088. [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Lopez A. F., Nicola N. A., Burgess A. W., Metcalf D., Battye F. L., Sewell W. A., Vadas M. Activation of granulocyte cytotoxic function by purified mouse colony-stimulating factors. J Immunol. 1983 Dec;131(6):2983–2988. [PubMed] [Google Scholar]
  7. Nicola N. A., Metcalf D., Johnson G. R., Burgess A. W. Separation of functionally distinct human granulocyte-macrophage colony-stimulating factors. Blood. 1979 Sep;54(3):614–627. [PubMed] [Google Scholar]
  8. Podestá E. J., Solano A. R., Attar R., Sánchez M. L., Molina y Vedia L. Receptor aggregation induced by antilutropin receptor antibody and biological response in rat testis Leydig cells. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3986–3990. doi: 10.1073/pnas.80.13.3986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Skubitz K. M., Zhen Y., August J. T. A human granulocyte-specific antigen characterized by use of monoclonal antibodies. Blood. 1983 Jan;61(1):19–26. [PubMed] [Google Scholar]
  10. Smith R. J., Bowman B. J., Iden S. S. Pepstatin A-induced degranulation and superoxide anion generation by human neutrophils. Clin Immunol Immunopathol. 1982 Jan;22(1):83–93. doi: 10.1016/0090-1229(82)90025-3. [DOI] [PubMed] [Google Scholar]
  11. Vadas M. A., David J. R., Butterworth A., Pisani N. T., Siongok T. A. A new method for the purification of human eosinophils and neutrophils, and a comparison of the ability of these cells to damage schistosomula of Schistosoma mansoni. J Immunol. 1979 Apr;122(4):1228–1236. [PubMed] [Google Scholar]
  12. Vadas M. A., Dessein A., Nicola N., David J. R. In vitro enhancement of the helminthotoxic capacity of human blood eosinophils. Aust J Exp Biol Med Sci. 1981 Dec;59(Pt 6):739–741. [PubMed] [Google Scholar]
  13. Vadas M. A., Nicola N. A., Metcalf D. Activation of antibody-dependent cell-mediated cytotoxicity of human neutrophils and eosinophils by separate colony-stimulating factors. J Immunol. 1983 Feb;130(2):795–799. [PubMed] [Google Scholar]
  14. Williams L. T., Snyderman R., Pike M. C., Lefkowitz R. J. Specific receptor sites for chemotactic peptides on human polymorphonuclear leukocytes. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1204–1208. doi: 10.1073/pnas.74.3.1204. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES