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. 2008 Jul 27;48(2):279–286. doi: 10.1007/s12088-008-0038-x

Diversity of ‘benzenetriol dioxygenase’ involved in p-nitrophenol degradation in soil bacteria

Debarati Paul 1, Neha Rastogi 1, Ulrich Krauss 2, Michael Schlomann 3, Gunjan Pandey 1, Janmejay Pandey 1, Anuradha Ghosh 1, Rakesh K Jain 1,✉
PMCID: PMC3450173  PMID: 23100721

Abstract

Ring hydroxylating dioxygenases (RHDOs) are one of the most important classes of enzymes featuring in the microbial metabolism of several xenobiotic aromatic compounds. One such RHDO is benzenetriol dioxygenase (BtD) which constitutes the metabolic machinery of microbial degradation of several mono- phenolic and biphenolic compounds including nitrophenols. Assessment of the natural diversity of benzenetriol dioxygenase (btd) gene sequence is of great significance from basic as well as applied study point of view. In the present study we have evaluated the gene sequence variations amongst the partial btd genes that were retrieved from microorganisms enriched for PNP degradation from pesticide contaminated agriculture soils. The gene sequence analysis was also supplemented with an in silico restriction digestion analysis. Furthermore, a phylogenetic analysis based on the deduced amino acid sequence(s) was performed wherein the evolutionary relatedness of BtD enzyme with similar aromatic dioxygenases was determined. The results obtained in this study indicated that this enzyme has probably undergone evolutionary divergence which largely corroborated with the taxonomic ranks of the host microorganisms.

Keywords: Benzenetriol dioxygenase, p-Nitrophenol, Phylogenetic analysis

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Glossary

BtD

Benzenetriol dioxygenase enzyme

btd

Benzenetriol dioxygenase gene

PNP

p-nitrophenol

Contributor Information

Ulrich Krauss, Email: u.krauss@fz-juelich.de.

Michael Schlomann, Phone: 49 3731 39 3739, FAX: 49 3731 39 3012, Email: michael.schloemann@ioez.tu-freiberg.de.

Rakesh K. Jain, Phone: +91 / 172 / 2695215, FAX: +91 / 172 / 2695085, Email: rkj@imtech.res.in

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