Skip to main content
NCBI home page
Indian Journal of Microbiology logoLink to Indian Journal of Microbiology
. 2008 Jul 27;48(2):243–251. doi: 10.1007/s12088-008-0035-0

Identification of a bacterial strain isolated from the liver of a laboratory mouse as Microbacterium paraoxydans and emended description of the species Microbacterium paraoxydans Laffineur et al 2003

Sandra Buczolits 1, Peter Schumann 2, Maria Valens 3, Ramón Rosselló-Mora 3, Hans-Jürgen Busse 1,✉
PMCID: PMC3450181  PMID: 23100717

Abstract

A rod-shaped, Gram-positive bacterial strain, designated C57-33, was isolated from the liver of the laboratory mouse strain C57Bl/6J and characterised by a polyphasic approach. 16S rRNA gene sequence similarity placed strain C57-33 in the genus Microbacterium with Microbacterium paraoxydans CF36T as the next relative (99.9% sequence similarity). Major fatty acids ai-C15:0, i-C16:0 and ai-C17:0 and peptidoglycan type B2β with ornithine as the diagnostic cell-wall diamino acid and glycolyl residues were in agreement with the description of Microbacterium paraoxydans. The quinone system of C57-33 (major menaquinones MK-12 and MK-11) and polar lipid profile (major polar lipids diphosphatidyl glycerol, phosphatidyl glycerol and two unknown glycolipids) were in accordance with those of Microbacterium paraoxydans strains CF36T, CF7 and CF40 which were analysed in this study as well. The results of biochemical/physiological characterisation, DNA-DNA hybridization, MALDI-TOF mass spectrometry of cell extracts and comparison of protein patterns after SDS-PAGE demonstrated that our isolate C57-33 (= DSM 15461) is a strain of the species Microbacterium paraoxydans. Based on new characteristics such as quinone system, polar lipid profile and physiological traits analysed for strain C57-33, the type strain of Microbacterium paraoxydans and some additional strains an emended description of the species Microbacterium paraoxydans is provided.

Keywords: Microbacterium paraoxydans, 16S rDNA sequence, chemotaxonomy, biochemical/physiological traits, emended description

Full Text

The Full Text of this article is available as a PDF (781.3 KB).

References

  • 1.Takeuchi M., Hatano K. Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium. Int J Syst Bacteriol. 1998;48:973–982. doi: 10.1099/00207713-48-3-739. [DOI] [PubMed] [Google Scholar]
  • 2.Behrendt U., Ulrich A., Schumann P. Description of Microbacterium foliorum sp. nov. and Microbacterium phyllosphaerae sp. nov., isolated from the phyllosphere of grasses and the surface litter after mulching the sward, and reclassification of Aureobacterium resistens (Funke et al. 1998) as Microbacterium resistens comb. nov. Int J Syst Evol Microbiol. 2001;51:1267–1276. doi: 10.1099/00207713-51-4-1267. [DOI] [PubMed] [Google Scholar]
  • 3.Richert K., Brambilla E., Stackebrandt E. The phylogenetic significance of peptidoglycan types: Molecular analysis of the genera Microbacterium and Aureobacterium based upon sequence comparisons of gyrB, rpoB, recA, and 16S rRNA genes. System Appl Microbiol. 2007;30:102–108. doi: 10.1016/j.syapm.2006.04.001. [DOI] [PubMed] [Google Scholar]
  • 4.Fox J.G., Dewhirst F.E., Tully J.G., Paster B.J., Yan L., Taylor N.S., Collins M.J., Jr, Gorelick P.L., Ward J.M. Helicobacter hepaticus sp. nov., a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice. J Clin Microbiol. 1994;32:1238–1245. doi: 10.1128/jcm.32.5.1238-1245.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Buczolits S., Schumann P., Weidler G., Radax C., Busse H.J. Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain. Int J Syst Evol Microbiol. 2003;53:1955–1960. doi: 10.1099/ijs.0.02728-0. [DOI] [PubMed] [Google Scholar]
  • 6.Moaledji K. Comparison of gram-staining and alternate methods, KOH test and aminopeptidase activity in aquatic bacteria: their application to numerical taxonomy. J Microbiol Meth. 1986;5:303–310. doi: 10.1016/0167-7012(86)90056-4. [DOI] [Google Scholar]
  • 7.Smibert R.M., Krieg N.R. Phenotypic characterisation. In: Gerhardt P., Murray R.G.E., Woods W.A., Krieg N.R., editors. Methods for General and Molecular Bacteriology. Washington: American Society for Microbiology; 1994. pp. 607–654. [Google Scholar]
  • 8.Buczolits S., Denner E.B.M., Vybiral D., Wieser M., Kämpfer P., Busse H.-J. Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov. Int J Syst Evol Microbiol. 2002;52:445–456. doi: 10.1099/00207713-52-2-445. [DOI] [PubMed] [Google Scholar]
  • 9.Zlamala C., Schumann P., Kämpfer P., Rossello-Mora R., Lubitz W., Busse H.-J. Agrococcus baldri sp. nov., isolated from the air in the „Virgilkapelle” in Vienna. Int J Syst Evol Microbiol. 2002;52:1211–1216. doi: 10.1099/ijs.0.01975-0. [DOI] [PubMed] [Google Scholar]
  • 10.Lane D.J. 16S/23S rRNA sequencing. In: Stackebrandt E., Goodfellow M., editors. Nucleic Acid Techniques in Bacterial Systematics. Chichester: Wiley; 1991. pp. 115–175. [Google Scholar]
  • 11.Wieser M., Schumann P., Martin K., Altenburger P., Burghardt J., Lubitz W., Busse H.J. Agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria) Int J Syst Bacteriol. 1999;49:1165–1170. doi: 10.1099/00207713-49-3-1165. [DOI] [PubMed] [Google Scholar]
  • 12.Middendorf L.R., Bruce J.C., Bruce R.C., Eckles R.D., Grone D.L., Roemer S.C., Sloniker G.D., Steffens D.L., Sutter S.L., Brumbaugh J.A., Patonay G. Continuous, on-line DNA sequencing using a versatile infrared laser scanner electrophoresis apparatus. Electrophoresis. 1992;13:487–494. doi: 10.1002/elps.11501301103. [DOI] [PubMed] [Google Scholar]
  • 13.Pearson W., Lipman D.J. Improved tools for biological sequence comparison. Proc Natl Acad Sci USA. 1988;85:2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Wieser M., Busse H.-J. Rapid identification of Staphylococcus epidermidis. Int J Syst Evol Microbiol. 2000;50:1087–1093. doi: 10.1099/00207713-50-3-1087. [DOI] [PubMed] [Google Scholar]
  • 15.Bruce J.L. Automated system rapidly identifies and characterizes microorganisms in food. Food Technol. 1996;50:77–81. [Google Scholar]
  • 16.Tindall B.J. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. System Appl Microbiol. 1990;13:128–130. [Google Scholar]
  • 17.Ventosa A., Marquez M.C., Kocur M., Tindall B.J. Comparative study of “Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus. Int J Syst Bacteriol. 1993;43:245–248. doi: 10.1099/00207713-43-2-245. [DOI] [PubMed] [Google Scholar]
  • 18.Stead D.E., Sellwood J.E., Wilson J., Viney I. Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. J Appl Bacteriol. 1992;72:315–321. [Google Scholar]
  • 19.Groth I., Schumann P., Weiss N., Martin K., Rainey F.A. Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol. 1996;46:234–239. doi: 10.1099/00207713-46-1-234. [DOI] [PubMed] [Google Scholar]
  • 20.Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. Classification of bacteria isolated from a medieval wall painting. J Biotechnol. 1996;47:39–52. doi: 10.1016/0168-1656(96)01376-4. [DOI] [Google Scholar]
  • 21.Schleifer K.H., Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev. 1972;36:407–477. doi: 10.1128/br.36.4.407-477.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Groth I., Schumann P., Rainey F.A., Martin K., Schuetze B., Augsten K. Bogoriella caseilytica gen. nov., sp. nov., a new alkaliphilic actinomycete from a soda lake in Africa. Int J Syst Bacteriol. 1997;47:788–794. doi: 10.1099/00207713-47-3-788. [DOI] [PubMed] [Google Scholar]
  • 23.Ziemke F., Höfle M.G., Lalucat J., Rossello-Mora R. Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol. 1998;48:179–186. doi: 10.1099/00207713-48-1-179. [DOI] [PubMed] [Google Scholar]
  • 24.Brosius J., Palmer M.L., Kennedy P.J., Noller H.F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA. 1978;75:4801–4805. doi: 10.1073/pnas.75.10.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Laffineur K., Avesani V., Cornu G., Charlier J., Janssens M., Wauters G., Delmée M. Bacteremia due to a novel Microbacterium species in a patient with leukemia and description of Microbacterium paraoxydans sp. nov. J Clin Microbiol. 2003;41:2242–2246. doi: 10.1128/JCM.41.5.2242-2246.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Maier T., Kostrzewa M. Fast and reliable MALDITOF MS-based microorganism identification. Chemistry Today. 2007;25:68–71. [Google Scholar]
  • 27.Kroppenstedt R.M., Mayilraj S., Wink J.M., Kallow W., Schumann P., Secondini C., Stackebrandt E. Eight new species of the genus Micromonospora, Micromonospora citrea sp. nov., Micromonospora echinaurantiaca sp. nov., Micromonospora echinofusca sp. nov., Micromonospora fulviviridis sp. nov., Micromonospora inyonensis sp. nov., Micromonospora peucetia sp. nov., Micromonospora sagamiensis sp. nov., and Micromonospora viridifaciens sp. nov. System Appl Microbiol. 2005;28:328–339. doi: 10.1016/j.syapm.2004.12.011. [DOI] [PubMed] [Google Scholar]
  • 28.Stackebrandt E., Päuker O., Erhard M. Grouping Myxococci (Corallococcus) strains by matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectrometry: Comparison with gene sequence phylogenies. Curr Microbiol. 2005;50:71–77. doi: 10.1007/s00284-004-4395-3. [DOI] [PubMed] [Google Scholar]
  • 29.Schippers A., Bosecker K., Spröer C., Schumann P. Microbacterium oleivorans sp. nov. and Microbacterium hydrocarbonoxydans sp. nov., novel crude-oil-degrading Grampositive bacteria. J Syst Evol Microbiol. 2005;55:655–660. doi: 10.1099/ijs.0.63305-0. [DOI] [PubMed] [Google Scholar]
  • 30.Kageyama A., Takahashi Y., Matsuo Y., Kasai H., Shizuri Y., Omura S. Microbacterium sediminicola sp. nov. and Microbacterium marinilacus sp. nov., isolated from marine environments. Int J Syst Evol Microbiol. 2007;57:2355–2359. doi: 10.1099/ijs.0.65038-0. [DOI] [PubMed] [Google Scholar]
  • 31.Zlamala C., Schumann P., Kämpfer P., Valens M., Rossello-Mora R., Lubitz W., Busse H.-J. Microbacterium aerolatum sp. nov., isolated from the air in the ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol. 2002;52:1229–1234. doi: 10.1099/ijs.0.02013-0. [DOI] [PubMed] [Google Scholar]
  • 32.Yokota A., Takeuchi M., Sakane T., Weiss N. Proposal of six new species in the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelianski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov. Int J Syst Bacteriol. 1993;43:555–564. doi: 10.1099/00207713-43-3-555. [DOI] [PubMed] [Google Scholar]

Articles from Indian Journal of Microbiology are provided here courtesy of Springer

RESOURCES