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. 1984 Mar;81(6):1854–1858. doi: 10.1073/pnas.81.6.1854

Corticotropin-releasing factor-immunoreactive neurons of the paraventricular nucleus become vasopressin positive after adrenalectomy.

J Z Kiss, E Mezey, L Skirboll
PMCID: PMC345020  PMID: 6608732

Abstract

The immunoperoxidase technique was used to study the effect of adrenalectomy on vasopressin (VP) immunoreactivity in the hypothalamic paraventricular nucleus of rat. In control animals, relatively few VP-immunostained parvocellular neurons were found in addition to a large population of magnocellular VP neurons. Seven to 14 days after bilateral adrenalectomy, VP immunostaining increased markedly in specific subdivisions of the paraventricular nucleus. In contrast to normal animals, VP immunoreactivity was localized in a large number of parvocellular neurons. Colchicine treatment, on the other hand, did not significantly increase the number of VP-immunostained parvocellular neurons found in control rats. These observations suggest that adrenalectomy increases the number of VP-positive neurons and appears to increase the intensity of VP immunoreactivity specifically in parvocellular neurons. VP parvocellular neurons are confined to those paraventricular nucleus subdivisions that are known to project to the external zone of the median eminence. Moreover, their distribution pattern is very similar, if not identical, to that of the corticotropin-releasing factor (CRF) immunoreactive cells. Parvocellular neurons on adjacent thin sections could be stained for both CRF and VP. Thus, adrenalectomy seems to increase VP staining in CRF immunoreactive parvocellular neurons, which innervate the external zone of the median eminence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anhut H., Meyer D. K., Knepel W. Cholecystokinin-like immunoreactivity of rat medial basal hypothalamus: investigations on a possible hypophysiotropic function. Neuroendocrinology. 1983 Feb;36(2):119–124. doi: 10.1159/000123447. [DOI] [PubMed] [Google Scholar]
  2. Antunes J. L., Carmel P. W., Zimmerman E. A. Projections from the paraventricular nucleus to the zona externa of the median eminence of the rhesus monkey: an immunohistochemical study. Brain Res. 1977 Nov 25;137(1):1–10. doi: 10.1016/0006-8993(77)91009-5. [DOI] [PubMed] [Google Scholar]
  3. Armstrong W. E., Warach S., Hatton G. I., McNeill T. H. Subnuclei in the rat hypothalamic paraventricular nucleus: a cytoarchitectural, horseradish peroxidase and immunocytochemical analysis. Neuroscience. 1980;5(11):1931–1958. doi: 10.1016/0306-4522(80)90040-8. [DOI] [PubMed] [Google Scholar]
  4. Brinkmann H., Bock R. Influence of various corticoids on the augmentation of "Gomori-positive" granules in the median eminence of the rat following adrenalectomy. Naunyn Schmiedebergs Arch Pharmacol. 1973;280(1):49–62. doi: 10.1007/BF00505354. [DOI] [PubMed] [Google Scholar]
  5. Chateau M., Marchetti J., Burlet A., Boulange M. Evidence of vasopressin in adenohypophysis: research into its role in corticotrope activity. Neuroendocrinology. 1979;28(1):25–35. doi: 10.1159/000122841. [DOI] [PubMed] [Google Scholar]
  6. Dierickx K., Vandesande F., de Mey J. Identification, in the external region of the rat median eminence, of separate neurophysin-vasopressin and neurophysin-oxytocin containing nerve fibres. Cell Tissue Res. 1976 May 6;168(2):141–151. doi: 10.1007/BF00215873. [DOI] [PubMed] [Google Scholar]
  7. Gillies G. E., Linton E. A., Lowry P. J. Corticotropin releasing activity of the new CRF is potentiated several times by vasopressin. Nature. 1982 Sep 23;299(5881):355–357. doi: 10.1038/299355a0. [DOI] [PubMed] [Google Scholar]
  8. Gillies G., Lowry P. J. Corticotrophin releasing activity in extracts of the stalk median eminence of Brattleboro rats. J Endocrinol. 1980 Jan;84(1):65–73. doi: 10.1677/joe.0.0840065. [DOI] [PubMed] [Google Scholar]
  9. Gillies G., Lowry P. Corticotrophin releasing factor may be modulated vasopressin. Nature. 1979 Mar 29;278(5703):463–464. doi: 10.1038/278463a0. [DOI] [PubMed] [Google Scholar]
  10. Kahn D., Abrams G. M., Zimmerman E. A., Carraway R., Leeman S. E. Neurotensin neurons in the rat hypothalamus: an immunocytochemical study. Endocrinology. 1980 Jul;107(1):47–54. doi: 10.1210/endo-107-1-47. [DOI] [PubMed] [Google Scholar]
  11. Lechan R. M., Nestler J. L., Jacobson S., Reichlin S. The hypothalamic 'tuberoinfundibular' system of the rat as demonstrated by horseradish peroxidase (HRP) microiontophoresis. Brain Res. 1980 Aug 11;195(1):13–27. doi: 10.1016/0006-8993(80)90862-8. [DOI] [PubMed] [Google Scholar]
  12. Oliver C., Mical R. S., Porter J. C. Hypothalamic-pituitary vasculature: evidence for retrograde blood flow in the pituitary stalk. Endocrinology. 1977 Aug;101(2):598–604. doi: 10.1210/endo-101-2-598. [DOI] [PubMed] [Google Scholar]
  13. Pittman Q. J., Blume H. W., Renaud L. P. Connections of the hypothalamic paraventricular nucleus with the neurohypophysis, median eminence, amygdala, lateral septum and midbrain periaqueductal gray: an electrophysiological study in the rat. Brain Res. 1981 Jun 29;215(1-2):15–28. doi: 10.1016/0006-8993(81)90488-1. [DOI] [PubMed] [Google Scholar]
  14. Pittman Q. J., Blume H. W., Renaud L. P. Electrophysiological indications that individual hypothalamic neurons innervate both median eminence and neurohypophysis. Brain Res. 1978 Nov 24;157(2):364–368. doi: 10.1016/0006-8993(78)90043-4. [DOI] [PubMed] [Google Scholar]
  15. Recht L. D., Hoffman D. L., Haldar J., Silverman A. J., Zimmerman E. A. Vasopressin concentrations in hypophysial portal plasma: insignificant reduction following removal of the posterior pituitary gland. Neuroendocrinology. 1981 Aug;33(2):88–90. doi: 10.1159/000123208. [DOI] [PubMed] [Google Scholar]
  16. Rhodes C. H., Morrell J. I., Pfaff D. W. Immunohistochemical analysis of magnocellular elements in rat hypothalamus: distribution and numbers of cells containing neurophysin, oxytocin, and vasopressin. J Comp Neurol. 1981 May 1;198(1):45–64. doi: 10.1002/cne.901980106. [DOI] [PubMed] [Google Scholar]
  17. Rivier C., Vale W. Interaction of corticotropin-releasing factor and arginine vasopressin on adrenocorticotropin secretion in vivo. Endocrinology. 1983 Sep;113(3):939–942. doi: 10.1210/endo-113-3-939. [DOI] [PubMed] [Google Scholar]
  18. Rivier C., Vale W. Modulation of stress-induced ACTH release by corticotropin-releasing factor, catecholamines and vasopressin. Nature. 1983 Sep 22;305(5932):325–327. doi: 10.1038/305325a0. [DOI] [PubMed] [Google Scholar]
  19. Roth K. A., Weber E., Barchas J. D. Immunoreactive corticotropin releasing factor (CRF) and vasopressin are colocalized in a subpopulation of the immunoreactive vasopressin cells in the paraventricular nucleus of the hypothalamus. Life Sci. 1982 Oct 18;31(16-17):1857–1860. doi: 10.1016/0024-3205(82)90228-4. [DOI] [PubMed] [Google Scholar]
  20. Russell J. T., Brownstein M. J., Gainer H. [35S]cysteine labeled peptides transported to the neurohypophyses of adrenalectomized, lactating, and Brattleboro rats. Brain Res. 1980 Nov 10;201(1):227–234. doi: 10.1016/0006-8993(80)90791-x. [DOI] [PubMed] [Google Scholar]
  21. Sawchenko P. E., Swanson L. W. Immunohistochemical identification of neurons in the paraventricular nucleus of the hypothalamus that project to the medulla or to the spinal cord in the rat. J Comp Neurol. 1982 Mar 1;205(3):260–272. doi: 10.1002/cne.902050306. [DOI] [PubMed] [Google Scholar]
  22. Seybold V., Elde R., Hökfelt T. Terminals of reserpine-sensitive vasopressin-neurophysin neurons in the external layer of the rat median eminence. Endocrinology. 1981 May;108(5):1803–1809. doi: 10.1210/endo-108-5-1803. [DOI] [PubMed] [Google Scholar]
  23. Silverman A. J., Hoffman D., Gadde C. A., Krey L. C., Zimmermann E. A. Adrenal steroid inhibition of the vasopressin-neurophysin neurosecretory system to the median eminence of the rat. Differential effects of corticosterone and deoxycorticosterone administration after adrenalectomy. Neuroendocrinology. 1981 Mar;32(3):129–133. doi: 10.1159/000123144. [DOI] [PubMed] [Google Scholar]
  24. Stillman M. A., Recht L. D., Rosario S. L., Seif S. M., Robinson A. G., Zimmerman E. A. The effects of adrenalectomy and glucocorticoid replacement on vasopressin and vasopressin-neurophysin in the zona externa of the median eminence of the rat. Endocrinology. 1977 Jul;101(1):42–49. doi: 10.1210/endo-101-1-42. [DOI] [PubMed] [Google Scholar]
  25. Swanson L. W., Kuypers H. G. The paraventricular nucleus of the hypothalamus: cytoarchitectonic subdivisions and organization of projections to the pituitary, dorsal vagal complex, and spinal cord as demonstrated by retrograde fluorescence double-labeling methods. J Comp Neurol. 1980 Dec 1;194(3):555–570. doi: 10.1002/cne.901940306. [DOI] [PubMed] [Google Scholar]
  26. Swanson L. W., Sawchenko P. E., Rivier J., Vale W. W. Organization of ovine corticotropin-releasing factor immunoreactive cells and fibers in the rat brain: an immunohistochemical study. Neuroendocrinology. 1983;36(3):165–186. doi: 10.1159/000123454. [DOI] [PubMed] [Google Scholar]
  27. Vandesande F., Dierickx K., De Mey J. The origin of the vasopressinergic and oxytocinergic fibres of the external region of the median eminence of the rat hypophysis. Cell Tissue Res. 1977 Jun 13;180(4):443–452. doi: 10.1007/BF00220167. [DOI] [PubMed] [Google Scholar]
  28. Wiegand S. J., Price J. L. Cells of origin of the afferent fibers to the median eminence in the rat. J Comp Neurol. 1980 Jul 1;192(1):1–19. doi: 10.1002/cne.901920102. [DOI] [PubMed] [Google Scholar]
  29. Yates F. E., Russell S. M., Dallman M. F., Hodge G. A., McCann S. M., Dhariwal A. P. Potentiation by vasopressin of corticotropin release induced by corticotropin-releasing factor. Endocrinology. 1971 Jan;88(1):3–15. doi: 10.1210/endo-88-1-3. [DOI] [PubMed] [Google Scholar]
  30. Zimmerman E. A., Carmel P. W., Husain M. K., Ferin M., Tannenbaum M., Frantz A. G., Robinson A. G. Vasopressin and neurophysin: high concentrations in monkey hypophyseal portal blood. Science. 1973 Nov 20;182(4115):925–927. doi: 10.1126/science.182.4115.925. [DOI] [PubMed] [Google Scholar]
  31. Zimmerman E. A., Hsu K. C., Robinson A. G., Carmel P. W., Frantz A. G., Tannenbaum M. Studies of neurophysin secreting neurons with immunoperoxidase techniques employing antibody to bovine neurophysin. I. Light microscopic findings in monkey and bovine tissues. Endocrinology. 1973 Mar;92(3):931–940. doi: 10.1210/endo-92-3-931. [DOI] [PubMed] [Google Scholar]

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