Abstract
An unconventional nutrient medium, distillery spent wash (1:3) diluted) was used to produce di-rhamnolipid biosurfactant by Pseudomonas aeruginosa strain BS2. This research further assessed the potential of the biosurfactant as a washing agent for metal removal from multimetal contaminated soil (Cr-940 ppm; Pb-900 ppm; Cd-430 ppm; Ni-880 ppm; Cu-480 ppm). Out of the treatments of contaminated soil with tap water and rhamnolipid biosurfactant, the latter was found to be potent in mobilization of metal and decontamination of contaminated soil. Within 36 hours of leaching study, di-rhamnolipid as compared to tap water facilitated 13 folds higher removal of Cr from the heavy metal spiked soil whereas removal of Pb and Cu was 9–10 and 14 folds higher respectively. Leaching of Cd and Ni was 25 folds higher from the spiked soil. This shows that leaching behavior of biosurfactant was different for different metals. The use of wastewater for production of biosurfactant and its efficient use in metal removal make it a strong applicant for bioremediation.
Keywords: Pseudomonas aeruginosa, Rhamnolipid Biosurfactant, Distillary waste, Metals, Column studies
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References
- 1.Kim J., Vipulanandan C. Removal of lead from contaminated water and clay soil using a biosurfactant. J Environ Engg. 2006;132:777–786. doi: 10.1061/(ASCE)0733-9372(2006)132:7(777). [DOI] [Google Scholar]
- 2.Neilson JW, Artiola JF and Maier RM (2003) Characterization of Lead Removal from Contaminated Soils by Nontoxic Soil-Washing. Agents J Environ Qual 32899–32908 [DOI] [PubMed]
- 3.Desai J., Banat I. Microbial production of surfactants and their commercial potential. Microbiol Mol Rev. 1997;61:47–64. doi: 10.1128/mmbr.61.1.47-64.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.Makkar R., Cameotra S. An update on the unconventional substrates for the biosurfactant production and their (new) applications. Appl Microbiol Biotechnol. 2002;58:428–434. doi: 10.1007/s00253-001-0924-1. [DOI] [PubMed] [Google Scholar]
- 5.Dubey K., Juwarkar A. Distillery and curd whey as viable alternative sources for biosurfactant production. World J Microbiol Biotech. 2001;17:61–69. doi: 10.1023/A:1016606509385. [DOI] [Google Scholar]
- 6.Cooper D.G., MacDonald C.R., Dull S.J.B., Kosaric N. Enhanced production of surfactin from B. subtilis by continuous product removal and metal cation additions. Appl Environ Microbiol. 1981;42:408–412. doi: 10.1128/aem.42.3.408-412.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Dubey K.V., Juwarkar A.A., Singh S.K. Adsorption-Desorption Process Using Wood-Based Activated Carbon for Recovery of Biosurfactant from Fermented Distillery Wastewater. Biotechnol Prog. 2005;21(3):860–867. doi: 10.1021/bp040012e. [DOI] [PubMed] [Google Scholar]
- 8.Robert M., Mercadé M.E., Bosch M.P., Parra J.L., Espuny M. J., Manresa M.A., Guinea J. Effect of the carbon source on biosurfactant production by Pseudomonas aeruginosa 44T1. Biotechnol Letters. 1989;11:871–874. doi: 10.1007/BF01026843. [DOI] [Google Scholar]
- 9.Thaniyavarn J., Chongchin A., Wanitsuksombut N., Thaniyavarn S., Pinphanichakarn P., Leepipatipiboon N., Morikawa M., Kanaya S. Biosurfactant production by Pseudomonas aeruginosa A41 using palm oil as carbon source. J General Appl Microbiol. 2006;52:215–222. doi: 10.2323/jgam.52.215. [DOI] [PubMed] [Google Scholar]
- 10.Benjamin M.M., Leckie J.O. Multiple-site adsorption of Cd, Cu, Zn and Pb on amorphous iron oxyhydroxide. J Colloid Interface Sci. 1981;79:209–221. doi: 10.1016/0021-9797(81)90063-1. [DOI] [Google Scholar]
- 11.Frazer L. Innovations: lipid lather removes metals. Environ Health Persp. 2000;108:320–323. doi: 10.2307/3434871. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12.Ochoa-Loza F.J., Artiola J.F., Maier R.M. Stability constants for the complexation of various metals with a rhamnolipid biosurfactant. J Environ Qual. 2001;30:479–485. doi: 10.2134/jeq2001.302479x. [DOI] [PubMed] [Google Scholar]
- 13.Wang S., Mulligan C.N. Rhamnolipid foam enhanced remediation of cadmium and nickel contaminated soil. Water Air Soil Pollut. 2004;157:315–330. doi: 10.1023/B:WATE.0000038904.91977.f0. [DOI] [Google Scholar]