Abstract
The development of the retinotectal projection has been studied by a new experimental approach combining antibodies against the nerve cell adhesion molecule (NCAM), and techniques for mapping neuronal pathways using rhodamine B isothiocyanate (RITC) crystals. Anti-NCAM Fab', which specifically inhibits neurite fasciculation, was injected into the eye cup of 4-day-old chicken embryos. After 4-6 days of development, a small RITC crystal was placed on the neural retina to stain selectively axons arising from a localized region. One day later the retina, optic nerve, and tectum were examined and the paths of the fluorescent retinal ganglion cell axons were traced. These studies have led to four observations: (i) The presence of anti-NCAM Fab' causes the axons to form a disordered nerve bundle at the optic fissure. (ii) Disorder produced in the optic nerve persists throughout the optic pathway up to the tectum. (iii) Many of the misrouted fibers growing on or near the tectal surface can at least partially correct their position. (iv) Late axons grow in straight tracks along other fibers and do not correct their position. Together the results suggest that formation of the retinotectal projection involves both reading of positional cues on the tectum by growth cones of early arriving retinal axons and the tracking of growth cones along preexisting fibers that normally belong to neighboring retinal ganglion cells.
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Selected References
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- Auerbach R., Kubai L., Knighton D., Folkman J. A simple procedure for the long-term cultivation of chicken embryos. Dev Biol. 1974 Dec;41(2):391–394. doi: 10.1016/0012-1606(74)90316-9. [DOI] [PubMed] [Google Scholar]
- Brackenbury R., Thiery J. P., Rutishauser U., Edelman G. M. Adhesion among neural cells of the chick embryo. I. An immunological assay for molecules involved in cell-cell binding. J Biol Chem. 1977 Oct 10;252(19):6835–6840. [PubMed] [Google Scholar]
- Crossland W. J., Cowan W. M., Rogers L. A., Kelly J. P. The specification of the retino-tectal projection in the chick. J Comp Neurol. 1974 May 15;155(2):127–164. doi: 10.1002/cne.901550202. [DOI] [PubMed] [Google Scholar]
- Fraser S. E., Hunt R. K. Retinotectal specificity: models and experiments in search of a mapping function. Annu Rev Neurosci. 1980;3:319–352. doi: 10.1146/annurev.ne.03.030180.001535. [DOI] [PubMed] [Google Scholar]
- Fujisawa H., Watanabe K., Tani N., Ibata Y. Retinotopic analysis of fiber pathways in amphibians. II. The frog Rana nigromaculata. Brain Res. 1981 Feb 9;206(1):21–26. doi: 10.1016/0006-8993(81)90097-4. [DOI] [PubMed] [Google Scholar]
- Gaze R. M., Keating M. J., Chung S. H. The evolution of the retinotectal map during development in Xenopus. Proc R Soc Lond B Biol Sci. 1974 Feb 12;185(1080):301–330. doi: 10.1098/rspb.1974.0021. [DOI] [PubMed] [Google Scholar]
- Gierer A. Development of projections between areas of the nervous system. Biol Cybern. 1981;42(1):69–78. doi: 10.1007/BF00335161. [DOI] [PubMed] [Google Scholar]
- Glass W. F., 2nd, Briggs R. C., Hnilica L. S. Identification of tissue-specific nuclear antigens transferred to nitrocellulose from polyacrylamide gels. Science. 1981 Jan 2;211(4477):70–72. doi: 10.1126/science.7003713. [DOI] [PubMed] [Google Scholar]
- Hoffman S., Sorkin B. C., White P. C., Brackenbury R., Mailhammer R., Rutishauser U., Cunningham B. A., Edelman G. M. Chemical characterization of a neural cell adhesion molecule purified from embryonic brain membranes. J Biol Chem. 1982 Jul 10;257(13):7720–7729. [PubMed] [Google Scholar]
- Horder T. J., Martin K. A. Morphogenetics as an alternative to chemospecificity in the formation of nerve connections. A review of literature, before 1978, concerning the control of growth of regenerating optic nerve fibres to specific locations in the optic tectum and a new interpretation based on contact guidance. Symp Soc Exp Biol. 1978;32:275–358. [PubMed] [Google Scholar]
- Marshak-Rothstein A., Fink P., Gridley T., Raulet D. H., Bevan M. J., Gefter M. L. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J Immunol. 1979 Jun;122(6):2491–2497. [PubMed] [Google Scholar]
- Rutishauser U., Edelman G. M. Effects of fasciculation on the outgrowth of neurites from spinal ganglia in culture. J Cell Biol. 1980 Nov;87(2 Pt 1):370–378. doi: 10.1083/jcb.87.2.370. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rutishauser U., Gall W. E., Edelman G. M. Adhesion among neural cells of the chick embryo. IV. Role of the cell surface molecule CAM in the formation of neurite bundles in cultures of spinal ganglia. J Cell Biol. 1978 Nov;79(2 Pt 1):382–393. doi: 10.1083/jcb.79.2.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rutishauser U., Hoffman S., Edelman G. M. Binding properties of a cell adhesion molecule from neural tissue. Proc Natl Acad Sci U S A. 1982 Jan;79(2):685–689. doi: 10.1073/pnas.79.2.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rutishauser U., Thiery J. P., Brackenbury R., Edelman G. M. Adhesion among neural cells of the chick embryo. III. Relationship of the surface molecule CAM to cell adhesion and the development of histotypic patterns. J Cell Biol. 1978 Nov;79(2 Pt 1):371–381. doi: 10.1083/jcb.79.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]